spacer gif spacer gif spacer gif spacer gif ARCHIVE ANNOUNCEMENT! spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search    

The fully linked HTML version of this article has now been published.
Development ePress online publication date 19 Nov 2003
doi: 10.1242/dev.00880

This Article
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
dev.00880v1
130/26/6431    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Bernhardt, C.
Right arrow Articles by Schiefelbein, J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Bernhardt, C.
Right arrow Articles by Schiefelbein, J.

Research article

The bHLH genes GLABRA3 (GL3) and ENHANCER OF GLABRA3 (EGL3) specify epidermal cell fate in the Arabidopsis root


Christine Bernhardt, Myeong Min Lee, Antonio Gonzalez, Fan Zhang, Alan Lloyd, and John Schiefelbein*
* Author for correspondence (e-mail: schiefel{at}umich.edu)

The position-dependent specification of the hair and non-hair cell types in the Arabidopsis root epidermis provides a simple model for the study of cell fate determination in plants. Several putative transcriptional regulators are known to influence this cell fate decision. Indirect evidence from studies with the maize R gene has been used to suggest that a bHLH transcription factor also participates in this process. We show that two Arabidopsis genes encoding bHLH proteins, GLABRA3 (GL3) and ENHANCER OF GLABRA3 (EGL3), act in a partially redundant manner to specify root epidermal cell fates. Plants homozygous for mutations in both genes fail to specify the non-hair cell type, whereas plants overexpressing either gene produce ectopic non-hair cells. We also find that these genes are required for appropriate transcription of the non-hair specification gene GL2 and the hair cell specification gene CPC, showing that GL3 and EGL3 influence both epidermal cell fates. Furthermore, we show that these bHLH proteins require a functional WER MYB protein for their action, and they physically interact with WER and CPC in the yeast two-hybrid assay. These results suggest a model in which GL3 and EGL3 act together with WER in the N cell position to promote the non-hair cell fate, whereas they interact with the incomplete MYB protein CPC in the H position, which blocks the non-hair pathway and leads to the hair cell fate.


This article has been cited by other articles:


Home page
 Plant Physiol.Home page
M. J. Jakoby, D. Falkenhan, M. T. Mader, G. Brininstool, E. Wischnitzki, N. Platz, A. Hudson, M. Hulskamp, J. Larkin, and A. Schnittger
Transcriptional Profiling of Mature Arabidopsis Trichomes Reveals That NOECK Encodes the MIXTA-Like Transcriptional Regulator MYB106
Plant Physiology, November 1, 2008; 148(3): 1583 - 1602.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
R. Tominaga, M. Iwata, R. Sano, K. Inoue, K. Okada, and T. Wada
Arabidopsis CAPRICE-LIKE MYB 3 (CPL3) controls endoreduplication and flowering development in addition to trichome and root hair formation
Development, April 1, 2008; 135(7): 1335 - 1345.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
S. Wang, S.-H. Kwak, Q. Zeng, B. E. Ellis, X.-Y. Chen, J. Schiefelbein, and J.-G. Chen
TRICHOMELESS1 regulates trichome patterning by suppressing GLABRA1 in Arabidopsis
Development, November 1, 2007; 134(21): 3873 - 3882.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
T. Ishida, S. Hattori, R. Sano, K. Inoue, Y. Shirano, H. Hayashi, D. Shibata, S. Sato, T. Kato, S. Tabata, et al.
Arabidopsis TRANSPARENT TESTA GLABRA2 Is Directly Regulated by R2R3 MYB Transcription Factors and Is Involved in Regulation of GLABRA2 Transcription in Epidermal Differentiation
PLANT CELL, August 1, 2007; 19(8): 2531 - 2543.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
R. Tominaga, M. Iwata, K. Okada, and T. Wada
Functional Analysis of the Epidermal-Specific MYB Genes CAPRICE and WEREWOLF in Arabidopsis
PLANT CELL, July 1, 2007; 19(7): 2264 - 2277.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
A. Feller, J. M. Hernandez, and E. Grotewold
An ACT-like Domain Participates in the Dimerization of Several Plant Basic-helix-loop-helix Transcription Factors
J. Biol. Chem., September 29, 2006; 281(39): 28964 - 28974.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
M. Kwasniewski and I. Szarejko
Molecular Cloning and Characterization of beta-Expansin Gene Related to Root Hair Formation in Barley
Plant Physiology, July 1, 2006; 141(3): 1149 - 1158.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
F. Quattrocchio, W. Verweij, A. Kroon, C. Spelt, J. Mol, and R. Koes
PH4 of Petunia Is an R2R3 MYB Protein That Activates Vacuolar Acidification through Interactions with Basic-Helix-Loop-Helix Transcription Factors of the Anthocyanin Pathway
PLANT CELL, May 1, 2006; 18(5): 1274 - 1291.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
M. T. Sweeney, M. J. Thomson, B. E. Pfeil, and S. McCouch
Caught Red-Handed: Rc Encodes a Basic Helix-Loop-Helix Protein Conditioning Red Pericarp in Rice
PLANT CELL, February 1, 2006; 18(2): 283 - 294.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
L. Dolan
Positional information and mobile transcriptional regulators determine cell pattern in the Arabidopsis root epidermis
J. Exp. Bot., January 1, 2006; 57(1): 51 - 54.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
T. Kurata, T. Ishida, C. Kawabata-Awai, M. Noguchi, S. Hattori, R. Sano, R. Nagasaka, R. Tominaga, Y. Koshino-Kimura, T. Kato, et al.
Cell-to-cell movement of the CAPRICE protein in Arabidopsis root epidermal cell differentiation
Development, December 15, 2005; 132(24): 5387 - 5398.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
K. H. Ryu, Y. H. Kang, Y.-h. Park, I. Hwang, J. Schiefelbein, and M. M. Lee
The WEREWOLF MYB protein directly regulates CAPRICE transcription during cell fate specification in the Arabidopsis root epidermis
Development, November 1, 2005; 132(21): 4765 - 4775.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
C.-R. Xu, C. Liu, Y.-L. Wang, L.-C. Li, W.-Q. Chen, Z.-H. Xu, and S.-N. Bai
Histone acetylation affects expression of cellular patterning genes in the Arabidopsis root epidermis
PNAS, October 4, 2005; 102(40): 14469 - 14474.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
L. Serna
Epidermal cell patterning and differentiation throughout the apical-basal axis of the seedling
J. Exp. Bot., August 1, 2005; 56(418): 1983 - 1989.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
Y. Koshino-Kimura, T. Wada, T. Tachibana, R. Tsugeki, S. Ishiguro, and K. Okada
Regulation of CAPRICE Transcription by MYB Proteins for Root Epidermis Differentiation in Arabidopsis
Plant Cell Physiol., June 1, 2005; 46(6): 817 - 826.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
C. Bernhardt, M. Zhao, A. Gonzalez, A. Lloyd, and J. Schiefelbein
The bHLH genes GL3 and EGL3 participate in an intercellular regulatory circuit that controls cell patterning in the Arabidopsis root epidermis
Development, January 15, 2005; 132(2): 291 - 298.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
G. Montiel, P. Gantet, C. Jay-Allemand, and C. Breton
Transcription Factor Networks. Pathways to the Knowledge of Root Development
Plant Physiology, November 1, 2004; 136(3): 3478 - 3485.
[Full Text] [PDF]

Home page
 Plant CellHome page
L. Serna
A Network of Interacting Factors Triggering Different Cell Fates
PLANT CELL, September 1, 2004; 16(9): 2258 - 2263.
[Full Text] [PDF]


© The Company of Biologists Ltd 2003