spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search    

The fully linked HTML version of this article has now been published.
Development ePress online publication date 23 Jun 2004
doi: 10.1242/dev.01244

This Article
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
dev.01244v1
dev.01244v2
131/15/3527    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Sonneville, R.
Right arrow Articles by Gönczy, P.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Sonneville, R.
Right arrow Articles by Gönczy, P.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?

Research article

zyg-11 and cul-2 regulate progression through meiosis II and polarity establishment in C. elegans


Rémi Sonneville and Pierre Gönczy*
* Author for correspondence (e-mail: pierre.gonczy{at}isrec.unil.ch)

The mechanisms that ensure coupling between meiotic cell cycle progression and subsequent developmental events, including specification of embryonic axes, are poorly understood. Here, we establish that zyg-11 and the cullin cul-2 promote the metaphase-to-anaphase transition and M phase exit at meiosis II in Caenorhabditis elegans. Our results indicate that ZYG-11 acts with a CUL-2-based E3 ligase that is essential at meiosis II and that functions redundantly with the anaphase-promoting complex/cyclosome at meiosis I. Our data also indicate that delayed M phase exit in zyg-11(RNAi) embryos is due to accumulation of the B type cyclin CYB-3. We demonstrate that PAR proteins and P granules become polarized in an inverted manner during the meiosis II delay resulting from zyg-11 or cul-2 inactivation, and that zyg-11 and cul-2 can regulate polarity establishment independently of a role in cell cycle progression. Furthermore, we find that microtubules appear dispensable for ectopic polarity during the meiosis II delay in zyg-11(RNAi) embryos, as well as for AP polarity during the first mitotic cell cycle in wild-type embryos. Our findings suggest a model in which a CUL-2-based E3 ligase promotes cell cycle progression and prevents polarity establishment during meiosis II, and in which the centrosome acts as a cue to polarize the embryo along the AP axis after exit from the meiotic cell cycle.
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter    What's this?


This article has been cited by other articles:


Home page
 J. Cell Sci.Home page
Y. Sasagawa, M. Otani, N. Higashitani, A. Higashitani, K. Sato, T. Ogura, and K. Yamanaka
Caenorhabditis elegans p97 controls germline-specific sex determination by controlling the TRA-1 level in a CUL-2-dependent manner
J. Cell Sci., October 15, 2009; 122(20): 3663 - 3672.
[Abstract] [Full Text] [PDF]

Home page
 Cold Spring Harb. Perspect. Biol.Home page
E. Munro and B. Bowerman
Cellular Symmetry Breaking during Caenorhabditis elegans Development
Cold Spring Harb Perspect Biol, October 1, 2009; 1(4): a003400 - a003400.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
Y. Budirahardja and P. Gonczy
Coupling the cell cycle to development
Development, September 1, 2009; 136(17): 2861 - 2872.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
M. Dorfman, J.-E. Gomes, S. O'Rourke, and B. Bowerman
Using RNA Interference to Identify Specific Modifiers of a Temperature-Sensitive, Embryonic-Lethal Mutation in the Caenorhabditis elegans Ubiquitin-Like Nedd8 Protein Modification Pathway E1-Activating Gene rfl-1
Genetics, August 1, 2009; 182(4): 1035 - 1049.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
J. R. Tenlen, J. N. Molk, N. London, B. D. Page, and J. R. Priess
MEX-5 asymmetry in one-cell C. elegans embryos requires PAR-4- and PAR-1-dependent phosphorylation
Development, November 15, 2008; 135(22): 3665 - 3675.
[Abstract] [Full Text] [PDF]

Home page
 JCBHome page
M.-C. Tsai and J. Ahringer
Microtubules are involved in anterior-posterior axis formation in C. elegans embryos
J. Cell Biol., November 5, 2007; 179(3): 397 - 402.
[Abstract] [Full Text] [PDF]

Home page
 JCBHome page
F. Motegi and G. Seydoux
Revisiting the role of microtubules in C. elegans polarity
J. Cell Biol., November 5, 2007; 179(3): 367 - 369.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
U. Wolke, E. A. Jezuit, and J. R. Priess
Actin-dependent cytoplasmic streaming in C. elegans oogenesis
Development, June 15, 2007; 134(12): 2227 - 2236.
[Abstract] [Full Text] [PDF]

Home page
 Genes Dev.Home page
S. E. Siegrist and C. Q. Doe
Microtubule-induced cortical cell polarity
Genes & Dev., March 1, 2007; 21(5): 483 - 496.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
R. Lyczak, L. Zweier, T. Group, M. A. Murrow, C. Snyder, L. Kulovitz, A. Beatty, K. Smith, and B. Bowerman
The puromycin-sensitive aminopeptidase PAM-1 is required for meiotic exit and anteroposterior polarity in the one-cell Caenorhabditis elegans embryo
Development, November 1, 2006; 133(21): 4281 - 4292.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
W. Chi and V. Reinke
Promotion of oogenesis and embryogenesis in the C. elegans gonad by EFL-1/DPL-1 (E2F) does not require LIN-35 (pRB)
Development, August 15, 2006; 133(16): 3147 - 3157.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
B. Bowerman and T. Kurz
Degrade to create: developmental requirements for ubiquitin-mediated proteolysis during early C. elegans embryogenesis
Development, March 1, 2006; 133(5): 773 - 784.
[Abstract] [Full Text] [PDF]


© The Company of Biologists Ltd 2004