spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search    

The fully linked HTML version of this article has now been published.
Development ePress online publication date 13 Mar 2008
doi: 10.1242/dev.014340

This Article
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
dev.014340v1
135/8/1513    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Endoh, M.
Right arrow Articles by Koseki, H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Endoh, M.
Right arrow Articles by Koseki, H.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?

Research article

Polycomb group proteins Ring1A/B are functionally linked to the core transcriptional regulatory circuitry to maintain ES cell identity


Mitsuhiro Endoh, Takaho A. Endo, Tamie Endoh, Yu-ichi Fujimura, Osamu Ohara, Tetsuro Toyoda, Arie P. Otte, Masaki Okano, Neil Brockdorff, Miguel Vidal, and Haruhiko Koseki*
* Author for correspondence (e-mail: koseki{at}rcai.riken.jp)

The Polycomb group (PcG) proteins mediate heritable silencing of developmental regulators in metazoans, participating in one of two distinct multimeric protein complexes, the Polycomb repressive complexes 1 (PRC1) and 2 (PRC2). Although PRC2 has been shown to share target genes with the core transcription network, including Oct3/4, to maintain embryonic stem (ES) cells, it is still unclear whether PcG proteins and the core transcription network are functionally linked. Here, we identify an essential role for the core PRC1 components Ring1A/B in repressing developmental regulators in mouse ES cells and, thereby, in maintaining ES cell identity. A significant proportion of the PRC1 target genes are also repressed by Oct3/4. We demonstrate that engagement of PRC1 at target genes is Oct3/4-dependent, whereas engagement of Oct3/4 is PRC1-independent. Moreover, upon differentiation induced by Gata6 expression, most of the Ring1A/B target genes are derepressed and the binding of Ring1A/B to their target loci is also decreased. Collectively, these results indicate that Ring1A/B-mediated Polycomb silencing functions downstream of the core transcriptional regulatory circuitry to maintain ES cell identity.


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter    What's this?


This article has been cited by other articles:


Home page
 Genes Dev.Home page
P. Yuan, J. Han, G. Guo, Y. L. Orlov, M. Huss, Y.-H. Loh, L.-P. Yaw, P. Robson, B. Lim, and H.-H. Ng
Eset partners with Oct4 to restrict extraembryonic trophoblast lineage potential in embryonic stem cells
Genes & Dev., November 1, 2009; 23(21): 2507 - 2520.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
B. Jin, B. Yao, J.-L. Li, C. R. Fields, A. L. Delmas, C. Liu, and K. D. Robertson
DNMT1 and DNMT3B Modulate Distinct Polycomb-Mediated Histone Modifications in Colon Cancer
Cancer Res., September 15, 2009; 69(18): 7412 - 7421.
[Abstract] [Full Text] [PDF]

Home page
 Cold Spring Harb Symp Quant BiolHome page
D. Pasini, A.P. Bracken, K. Agger, J. Christensen, K. Hansen, P.A.C. Cloos, and K. Helin
Regulation of Stem Cell Differentiation by Histone Methyltransferases and Demethylases
Cold Spring Harb Symp Quant Biol, November 6, 2008; (2008) sqb.2008.73.009v1.
[Abstract] [PDF]

Home page
 Cold Spring Harb Symp Quant BiolHome page
M.F. Cole and R.A. Young
Mapping Key Features of Transcriptional Regulatory Circuitry in Embryonic Stem Cells
Cold Spring Harb Symp Quant Biol, November 6, 2008; (2008) sqb.2008.73.027v1.
[Abstract] [PDF]

Home page
 J. Cell Sci.Home page
M. Endoh, T. A. Endo, T. Endoh, Y.-i. Fujimura, O. Ohara, T. Toyoda, A. P. Otte, M. Okano, N. Brockdorff, M. Vidal, et al.
Polycomb group proteins Ring1A/B are functionally linked to the core transcriptional regulatory circuitry to maintain ES cell identity
J. Cell Sci., April 15, 2008; 121(8): e806 - e806.
[Full Text]


© The Company of Biologists Ltd 2008