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Development ePress online publication date 2 Dec 2004
doi: 10.1242/dev.01555
Research article
Spermatogenesis from epiblast and primordial germ cells following transplantation into postnatal mouse testis
Shinichiro Chuma,
Mito Kanatsu-Shinohara,
Kimiko Inoue,
Narumi Ogonuki,
Hiromi Miki,
Shinya Toyokuni,
Mihoko Hosokawa,
Norio Nakatsuji,
Atsuo Ogura,
and
Takashi Shinohara*
* Author for correspondence (e-mail: takashi{at}mfour.med.kyoto-u.ac.jp)
Primordial germ cells (PGCs) are derived from a population of pluripotent epiblast cells in mice. However, little is known about when and how PGCs acquire the capacity to differentiate into functional germ cells, while keeping the potential to derive pluripotent embryonic germ cells and teratocarcinomas. In this investigation, we show that epiblast cells and PGCs can establish colonies of spermatogenesis after transfer into postnatal seminiferous tubules of surrogate infertile mice. Furthermore, we obtained normal fertile offspring by microinsemination using spermatozoa or spermatids derived from PGCs harvested from fetuses as early as 8.5 days post coitum. Thus, fetal male germ cell development is remarkably flexible, and the maturation process, from epiblast cells through PGCs to postnatal spermatogonia, can occur in the postnatal testicular environment. Primordial germ cell transplantation techniques will also provide a novel tool to assess the developmental potential of PGCs, such as those manipulated in vitro or recovered from embryos harboring lethal mutations.
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© The Company of Biologists Ltd 2004
