|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search | ||||
The fully linked HTML version of this article has now been published.
Formins are key regulators of actin nucleation and elongation. Diaphanous-related formins, the best-known subclass, are activated by Rho and play essential roles in cytokinesis. In cultured cells, Diaphanous-related formins also regulate cell adhesion, polarity and microtubules, suggesting that they may be key regulators of cell shape change and migration during development. However, their essential roles in cytokinesis hamper our ability to test this hypothesis. We used loss- and gain-of-function approaches to examine the role of Diaphanous in Drosophila morphogenesis. We found that Diaphanous has a dynamic expression pattern consistent with a role in regulating cell shape change. We used constitutively active Diaphanous to examine its roles in morphogenesis and its mechanisms of action. This revealed an unexpected role in regulating myosin levels and activity at adherens junctions during cell shape change, suggesting that Diaphanous helps coordinate adhesion and contractility of the underlying actomyosin ring. We tested this hypothesis by reducing Diaphanous function, revealing striking roles in stabilizing adherens junctions and inhibiting cell protrusiveness. These effects also are mediated through coordinated effects on myosin activity and adhesion, suggesting a common mechanism for Diaphanous action during morphogenesis.
This article has been cited by other articles:
Development ePress online publication date 6 Feb 2008
doi: 10.1242/dev.016337
This Article 
Full Text (PDF)
All Versions of this Article:
dev.016337v1
135/6/1005
most recent
Alert me when this article is cited
Alert me if a correction is posted
Services
Email this article to a friend
Similar articles in this journal
Similar articles in PubMed
Alert me to new issues of the journal
Download to citation manager
Citing Articles
Citing Articles via HighWire
Citing Articles via Google Scholar
Google Scholar
Articles by Homem, C. C.F.
Articles by Peifer, M.
Search for Related Content
PubMed
PubMed Citation
Articles by Homem, C. C.F.
Articles by Peifer, M.
Social Bookmarking
What's this?
Research article
Diaphanous regulates myosin and adherens junctions to control cell contractility and protrusive behavior during morphogenesis
* Author for correspondence (e-mail: peifer{at}unc.edu)
CiteULike Complore Connotea Del.icio.us Digg Reddit Technorati Twitter What's this?
M. Cavey and T. Lecuit
Molecular Bases of Cell-Cell Junctions Stability and Dynamics
Cold Spring Harb Perspect Biol,
November 1, 2009;
1(5):
a002998 - a002998.
[Abstract]
[Full Text]
[PDF]
S. J. Warner and G. D. Longmore
Cdc42 antagonizes Rho1 activity at adherens junctions to limit epithelial cell apical tension
J. Cell Biol.,
October 5, 2009;
187(1):
119 - 133.
[Abstract]
[Full Text]
[PDF]
S. J. Warner and G. D. Longmore
Distinct functions for Rho1 in maintaining adherens junctions and apical tension in remodeling epithelia
J. Cell Biol.,
June 15, 2009;
185(6):
1111 - 1125.
[Abstract]
[Full Text]
[PDF]
N. Gorfinkiel, G. B. Blanchard, R. J. Adams, and A. Martinez Arias
Mechanical control of global cell behaviour during dorsal closure in Drosophila
Development,
June 1, 2009;
136(11):
1889 - 1898.
[Abstract]
[Full Text]
[PDF]
R. L. Webb, M.-N. Zhou, and B. M. McCartney
A novel role for an APC2-Diaphanous complex in regulating actin organization in Drosophila
Development,
April 15, 2009;
136(8):
1283 - 1293.
[Abstract]
[Full Text]
[PDF]
S. Mulinari, M. P. Barmchi, and U. Hacker
DRhoGEF2 and Diaphanous Regulate Contractile Force during Segmental Groove Morphogenesis in the Drosophila Embryo
Mol. Biol. Cell,
May 1, 2008;
19(5):
1883 - 1892.
[Abstract]
[Full Text]
[PDF]
© The Company of Biologists Ltd 2008