spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search    

The fully linked HTML version of this article has now been published.
Development ePress online publication date 2 Feb 2005
doi: 10.1242/dev.01644

This Article
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
dev.01644v1
132/5/1093    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Crosnier, C.
Right arrow Articles by Lewis, J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Crosnier, C.
Right arrow Articles by Lewis, J.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?

Research article

Delta-Notch signalling controls commitment to a secretory fate in the zebrafish intestine


Cécile Crosnier, Neil Vargesson, Stephen Gschmeissner, Linda Ariza-McNaughton, Alastair Morrison, and Julian Lewis*
* Author for correspondence (e-mail: julian.lewis{at}cancer.org.uk)

The transparency of the juvenile zebrafish and its genetic advantages make it an attractive model for study of cell turnover in the gut. BrdU labelling shows that the gut epithelium is renewed in essentially the same way as in mammals: the villi are lined with non-dividing differentiated cells, while cell division is confined to the intervillus pockets. New cells produced in the pockets take about 4 days to migrate out to the tips of the villi, where they die. We have generated monoclonal antibodies to identify the absorptive and secretory cells in the epithelium, and we have used these antibodies to examine the part that Delta-Notch signalling plays in producing the diversity of intestinal cell types. Several Notch receptors and ligands are expressed in the gut. In particular, the Notch ligand DeltaD (Delta1 in the mouse) is expressed in cells of the secretory lineage. In an aei mutant, where DeltaD is defective, secretory cells are overproduced. In mind bomb (mib), where all Delta-Notch signalling is believed to be blocked, almost all the cells in the 3-day gut epithelium adopt a secretory character. Thus, secretory differentiation appears to be the default in the absence of Notch activation, and lateral inhibition mediated by Delta-Notch signalling is required to generate a balanced mixture of absorptive and secretory cells. These findings demonstrate the central role of Notch signalling in the gut stem-cell system and establish the zebrafish as a model for study of the mechanisms controlling renewal of gut epithelium.
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter    What's this?


This article has been cited by other articles:


Home page
 Mol Cancer ResHome page
A. M. Ghaleb, G. Aggarwal, A. B. Bialkowska, M. O. Nandan, and V. W. Yang
Notch Inhibits Expression of the Kruppel-Like Factor 4 Tumor Suppressor in the Intestinal Epithelium
Mol. Cancer Res., December 1, 2008; 6(12): 1920 - 1927.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
D. Raldua, D. Otero, M. Fabra, and J. Cerda
Differential localization and regulation of two aquaporin-1 homologs in the intestinal epithelia of the marine teleost Sparus aurata
Am J Physiol Regulatory Integrative Comp Physiol, March 1, 2008; 294(3): R993 - R1003.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
P. Hayward, T. Kalmar, and A. Martinez Arias
Wnt/Notch signalling and information processing during development
Development, February 1, 2008; 135(3): 411 - 424.
[Abstract] [Full Text] [PDF]

Home page
 Endocr. Rev.Home page
V. Bolos, J. Grego-Bessa, and J. L. de la Pompa
Notch Signaling in Development and Cancer
Endocr. Rev., May 1, 2007; 28(3): 339 - 363.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
R. Kageyama, T. Ohtsuka, and T. Kobayashi
The Hes gene family: repressors and oscillators that orchestrate embryogenesis
Development, April 1, 2007; 134(7): 1243 - 1251.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
Y. Liu, N. Pathak, A. Kramer-Zucker, and I. A. Drummond
Notch signaling controls the differentiation of transporting epithelia and multiciliated cells in the zebrafish pronephros
Development, March 15, 2007; 134(6): 1111 - 1122.
[Abstract] [Full Text] [PDF]

Home page
 Genes Dev.Home page
X. Zhu, J. Zhang, J. Tollkuhn, R. Ohsawa, E. H. Bresnick, F. Guillemot, R. Kageyama, and M. G. Rosenfeld
Sustained Notch signaling in progenitors is required for sequential emergence of distinct cell lineages during organogenesis
Genes & Dev., October 1, 2006; 20(19): 2739 - 2753.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
M. Bjerknes and H. Cheng
Gastrointestinal Stem Cells. II. Intestinal stem cells
Am J Physiol Gastrointest Liver Physiol, September 1, 2005; 289(3): G381 - G387.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
B. Z. Stanger, R. Datar, L. C. Murtaugh, and D. A. Melton
Direct regulation of intestinal fate by Notch
PNAS, August 30, 2005; 102(35): 12443 - 12448.
[Abstract] [Full Text] [PDF]

Home page
 Genes Dev.Home page
V. Zecchini, R. Domaschenz, D. Winton, and P. Jones
Notch signaling regulates the differentiation of post-mitotic intestinal epithelial cells
Genes & Dev., July 15, 2005; 19(14): 1686 - 1691.
[Abstract] [Full Text] [PDF]


© The Company of Biologists Ltd 2005