The fully linked HTML version of this article has now been published.
Development ePress online publication date 23 Feb 2005
doi: 10.1242/dev.01697
Research article
Roles of cell-adhesion molecules nectin 1 and nectin 3 in ciliary body development
Maiko Inagaki,
Kenji Irie,
Hiroyoshi Ishizaki,
Miki Tanaka-Okamoto,
Koji Morimoto,
Eiji Inoue,
Toshihisa Ohtsuka,
Jun Miyoshi,
and
Yoshimi Takai*
* Author for correspondence (e-mail: ytakai{at}molbio.med.osaka-u.ac.jp)
Nectins are Ca2+-independent immunoglobulin-like cell-cell-adhesion molecules consisting of four members. Nectins homophilically and heterophilically trans-interact to form a variety of cell-cell junctions, including cadherin-based adherens junctions in epithelial cells and fibroblasts in culture, synaptic junctions in neurons, and Sertoli cell-spermatid junctions in the testis, in cooperation with, or independently of, cadherins. To further explore the function of nectins, we generated nectin 1-/- and nectin 3-/- mice. Both nectin 1-/- and nectin 3-/- mice showed a virtually identical ocular phenotype, microphthalmia, accompanied by a separation of the apex-apex contact between the pigment and non-pigment cell layers of the ciliary epithelia. Immunofluorescence and immunoelectron microscopy revealed that nectin 1 and nectin 3, but not nectin 2, localized at the apex-apex junctions between the pigment and non-pigment cell layers of the ciliary epithelia. However, nectin 1-/- and nectin 3-/- mice showed no impairment of the apicolateral junctions between the pigment epithelia where nectin 1, nectin 2 and nectin 3 localized, or of the apicolateral junctions between the non-pigment epithelia where nectin 2 and nectin 3, but not nectin 1, localized. These results indicate that the heterophilic trans-interaction between nectin 1 and nectin 3 plays a sentinel role in establishing the apex-apex adhesion between the pigment and non-pigment cell layers of the ciliary epithelia that is essential for the morphogenesis of the ciliary body.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati 
Twitter What's this?
This article has been cited by other articles:
|
|
|
|
 
S. J. Kopp, G. Banisadr, K. Glajch, U. E. Maurer, K. Grunewald, R. J. Miller, P. Osten, and P. G. Spear
Infection of neurons and encephalitis after intracranial inoculation of herpes simplex virus requires the entry receptor nectin-1
PNAS,
October 20, 2009;
106(42):
17916 - 17920.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. Meyer, S. Seth, J. Albrecht, M. K. Maier, L. d. Pasquier, I. Ravens, L. Dreyer, R. Burger, M. Gramatzki, R. Schwinzer, et al.
CD96 Interaction with CD155 via Its First Ig-like Domain Is Modulated by Alternative Splicing or Mutations in Distal Ig-like Domains
J. Biol. Chem.,
January 23, 2009;
284(4):
2235 - 2244.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. J. Barron, S. J. Brookes, C. E. Draper, D. Garrod, J. Kirkham, R. C. Shore, and M. J. Dixon
The cell adhesion molecule nectin-1 is critical for normal enamel formation in mice
Hum. Mol. Genet.,
November 15, 2008;
17(22):
3509 - 3520.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
P.-N. Tsao, F. Chen, K. I. Izvolsky, J. Walker, M. A. Kukuruzinska, J. Lu, and W. V. Cardoso
{gamma}-Secretase Activation of Notch Signaling Regulates the Balance of Proximal and Distal Fates in Progenitor Cells of the Developing Lung
J. Biol. Chem.,
October 24, 2008;
283(43):
29532 - 29544.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
N. Kanzaki, H. Ogita, H. Komura, M. Ozaki, Y. Sakamoto, T. Majima, T. Ijuin, T. Takenawa, and Y. Takai
Involvement of the nectin-afadin complex in PDGF-induced cell survival
J. Cell Sci.,
June 15, 2008;
121(12):
2008 - 2017.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. H. Ch'ng, P. G. Spear, F. Struyf, and L. W. Enquist
Glycoprotein D-Independent Spread of Pseudorabies Virus Infection in Cultured Peripheral Nervous System Neurons in a Compartmented System
J. Virol.,
October 1, 2007;
81(19):
10742 - 10757.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Ooshio, N. Fujita, A. Yamada, T. Sato, Y. Kitagawa, R. Okamoto, S. Nakata, A. Miki, K. Irie, and Y. Takai
Cooperative roles of Par-3 and afadin in the formation of adherens and tight junctions
J. Cell Sci.,
July 15, 2007;
120(14):
2352 - 2365.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. Wakamatsu, H. Ogita, N. Okabe, K. Irie, M. Tanaka-Okamoto, H. Ishizaki, A. Ishida-Yamamoto, H. Iizuka, J. Miyoshi, and Y. Takai
Up-regulation of Loricrin Expression by Cell Adhesion Molecule Nectin-1 through Rap1-ERK Signaling in Keratinocytes
J. Biol. Chem.,
June 22, 2007;
282(25):
18173 - 18181.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
V. Hyenne, J. C Harf, M. Latz, B. Maro, U. Wolfrum, and M.-C. Simmler
Vezatin, a ubiquitous protein of adherens cell-cell junctions, is exclusively expressed in germ cells in mouse testis
Reproduction,
March 1, 2007;
133(3):
563 - 574.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
E. I. Surace, A. Strickland, R. A. Hess, D. H. Gutmann, and C. K. Naughton
Tslc1 (Nectin-Like Molecule-2) Is Essential for Spermatozoa Motility and Male Fertility
J Androl,
November 1, 2006;
27(6):
816 - 825.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Inagaki, K. Irie, H. Ishizaki, M. Tanaka-Okamoto, J. Miyoshi, and Y. Takai
Role of cell adhesion molecule nectin-3 in spermatid development.
Genes Cells,
September 1, 2006;
11(9):
1125 - 1132.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. Togashi, J. Miyoshi, T. Honda, T. Sakisaka, Y. Takai, and M. Takeichi
Interneurite affinity is regulated by heterophilic nectin interactions in concert with the cadherin machinery
J. Cell Biol.,
July 3, 2006;
174(1):
141 - 151.
[Abstract]
[Full Text]
[PDF]
|
|
|
© The Company of Biologists Ltd 2005
