The fully linked HTML version of this article has now been published.
Development ePress online publication date 27 Apr 2005
doi: 10.1242/dev.01850
Research article
Requirements of genetic interactions between Src42A, armadillo and shotgun, a gene encoding E-cadherin, for normal development in Drosophila
Mayuko Takahashi,
Fumitaka Takahashi,
Kumiko Ui-Tei,
Tetsuya Kojima,
and
Kaoru Saigo*
* Author for correspondence (e-mail: saigo{at}biochem.s.u-tokyo.ac.jp)
Src42A is one of the two Src homologs in Drosophila. Src42A protein accumulates at sites of cell-cell or cell-matrix adhesion. Anti-Engrailed antibody staining of Src42A protein-null mutant embryos indicated that Src42A is essential for proper cell-cell matching during dorsal closure. Src42A, which is functionally redundant to Src64, was found to interact genetically with shotgun, a gene encoding E-cadherin, and armadillo, a Drosophila 
-catenin. Immunoprecipitation and a pull-down assay indicated that Src42A forms a ternary complex with E-cadherin and Armadillo, and that Src42A binds to Armadillo repeats via a 14 amino acid region, which contains the major autophosphorylation site. The leading edge of Src mutant embryos exhibiting the dorsal open phenotype was frequently kinked and associated with significant reduction in E-cadherin, Armadillo and F-actin accumulation, suggesting that not only Src signaling but also Src-dependent adherens-junction stabilization would appear likely to be essential for normal dorsal closure. Src42A and Src64 were required for Armadillo tyrosine residue phosphorylation but Src activity may not be directly involved in Armadillo tyrosine residue phosphorylation at the adherens junction.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati 
Twitter What's this?
This article has been cited by other articles:
|
|
|
|
 
G. Ren, F. M. Helwani, S. Verma, R. W. McLachlan, S. A. Weed, and A. S. Yap
Cortactin Is a Functional Target of E-cadherin-activated Src Family Kinases in MCF7 Epithelial Monolayers
J. Biol. Chem.,
July 10, 2009;
284(28):
18913 - 18922.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
N. Gorfinkiel, G. B. Blanchard, R. J. Adams, and A. Martinez Arias
Mechanical control of global cell behaviour during dorsal closure in Drosophila
Development,
June 1, 2009;
136(11):
1889 - 1898.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. M. Coate, T. L. Swanson, and P. F. Copenhaver
Reverse Signaling by Glycosylphosphatidylinositol-Linked Manduca Ephrin Requires a Src Family Kinase to Restrict Neuronal Migration In Vivo
J. Neurosci.,
March 18, 2009;
29(11):
3404 - 3418.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Shindo, H. Wada, M. Kaido, M. Tateno, T. Aigaki, L. Tsuda, and S. Hayashi
Dual function of Src in the maintenance of adherens junctions during tracheal epithelial morphogenesis
Development,
April 1, 2008;
135(7):
1355 - 1364.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Vidal, S. Warner, R. Read, and R. L. Cagan
Differing Src Signaling Levels Have Distinct Outcomes in Drosophila
Cancer Res.,
November 1, 2007;
67(21):
10278 - 10285.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
N. Gorfinkiel and A. M. Arias
Requirements for adherens junction components in the interaction between epithelial tissues during dorsal closure in Drosophila
J. Cell Sci.,
September 15, 2007;
120(18):
3289 - 3298.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. W. McLachlan, A. Kraemer, F. M. Helwani, E. M. Kovacs, and A. S. Yap
E-Cadherin Adhesion Activates c-Src Signaling at Cell Cell Contacts
Mol. Biol. Cell,
August 1, 2007;
18(8):
3214 - 3223.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. J. Murray, C. M. Davidson, N. M. Hayward, and A. H. Brand
The Fes/Fer non-receptor tyrosine kinase cooperates with Src42A to regulate dorsal closure in Drosophila
Development,
August 15, 2006;
133(16):
3063 - 3073.
[Abstract]
[Full Text]
[PDF]
|
|
|
© The Company of Biologists Ltd 2005
