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Development ePress online publication date 1 Jun 2005
doi: 10.1242/dev.01864
Research article
Essential role of protein kinase B
(PKB/Akt3) in postnatal brain development but not in glucose homeostasis
Oliver Tschopp,
Zhong-Zhou Yang,
Daniela Brodbeck,
Bettina A. Dummler,
Maja Hemmings-Mieszczak,
Takashi Watanabe,
Thomas Michaelis,
Jens Frahm,
and
Brian A. Hemmings*
* Author for correspondence (e-mail: brian.hemmings{at}fmi.ch)
Protein kinase B is implicated in many crucial cellular processes, such as metabolism, apoptosis and cell proliferation. In contrast to Pkb
and Pkb
-deficient mice, Pkb
-/- mice are viable, show no growth retardation and display normal glucose metabolism. However, in adult Pkb mutant mice, brain size and weight are dramatically reduced by about 25%. In vivo magnetic resonance imaging confirmed the reduction of Pkb -/- brain volumes with a proportionally smaller ventricular system. Examination of the major brain structures revealed no anatomical malformations except for a pronounced thinning of white matter fibre connections in the corpus callosum. The reduction in brain weight of Pkb -/- mice is caused, at least partially, by a significant reduction in both cell size and cell number. Our results provide novel insights into the physiological role of PKB and suggest a crucial role in postnatal brain development.
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