spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search    

The fully linked HTML version of this article has now been published.
Development ePress online publication date 23 Jun 2005
doi: 10.1242/dev.01901

This Article
Right arrow Full Text (PDF)
Right arrowOA All Versions of this Article:
dev.01901v1
132/15/3381    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Reversade, B.
Right arrow Articles by De Robertis, E. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Reversade, B.
Right arrow Articles by De Robertis, E. M.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?

Research article

Depletion of Bmp2, Bmp4, Bmp7 and Spemann organizer signals induces massive brain formation in Xenopus embryos


Bruno Reversade, Hiroki Kuroda, Hojoon Lee, Ashley Mays, and Edward M. De Robertis*
* Author for correspondence (e-mail: ederobertis{at}mednet.ucla.edu)

To address the patterning function of the Bmp2, Bmp4 and Bmp7 growth factors, we designed antisense morpholino oligomers (MO) that block their activity in Xenopus laevis. Bmp4 knockdown was sufficient to rescue the ventralizing effects caused by loss of Chordin activity. Double Bmp4 and Bmp7 knockdown inhibited tail development. Triple Bmp2/Bmp4/Bmp7 depletion further compromised trunk development but did not eliminate dorsoventral patterning. Unexpectedly, we found that blocking Spemann organizer formation by UV treatment or {beta}-Catenin depletion caused BMP inhibition to have much more potent effects, abolishing all ventral development and resulting in embryos having radial central nervous system (CNS) structures. Surprisingly, dorsal signaling molecules such as Chordin, Noggin, Xnr6 and Cerberus were not re-expressed in these embryos. We conclude that BMP inhibition is sufficient for neural induction in vivo, and that in the absence of ventral BMPs, Spemann organizer signals are not required for brain formation.
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter    What's this?


This article has been cited by other articles:


Home page
 DevelopmentHome page
D. Umulis, M. B. O'Connor, and S. S. Blair
The extracellular regulation of bone morphogenetic protein signaling
Development, November 15, 2009; 136(22): 3715 - 3728.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
M. Saina, G. Genikhovich, E. Renfer, and U. Technau
BMPs and Chordin regulate patterning of the directive axis in a sea anemone
PNAS, November 3, 2009; 106(44): 18592 - 18597.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
T. J. Geach and L. Dale
Molecular Determinants of Xolloid Action in Vivo
J. Biol. Chem., October 3, 2008; 283(40): 27057 - 27063.
[Abstract] [Full Text] [PDF]

Home page
 Hum Mol GenetHome page
J. Gaulden and J. F. Reiter
Neur-ons and neur-offs: regulators of neural induction in vertebrate embryos and embryonic stem cells
Hum. Mol. Genet., April 15, 2008; 17(R1): R60 - R66.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
J. R. Hens, P. Dann, J.-P. Zhang, S. Harris, G. W. Robinson, and J. Wysolmerski
BMP4 and PTHrP interact to stimulate ductal outgrowth during embryonic mammary development and to inhibit hair follicle induction
Development, March 15, 2007; 134(6): 1221 - 1230.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
M. v. d. Zee, O. Stockhammer, C. v. Levetzow, R. N. d. Fonseca, and S. Roth
Sog/Chordin is required for ventral-to-dorsal Dpp/BMP transport and head formation in a short germ insect
PNAS, October 31, 2006; 103(44): 16307 - 16312.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
J. Heasman
Patterning the early Xenopus embryo.
Development, April 1, 2006; 133(7): 1205 - 1217.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
G. Bellipanni, M. Varga, S. Maegawa, Y. Imai, C. Kelly, A. P. Myers, F. Chu, W. S. Talbot, and E. S. Weinberg
Essential and opposing roles of zebrafish {beta}-catenins in the formation of dorsal axial structures and neurectoderm
Development, April 1, 2006; 133(7): 1299 - 1309.
[Abstract] [Full Text] [PDF]


© The Company of Biologists Ltd 2005