spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search    

The fully linked HTML version of this article has now been published.
Development ePress online publication date 20 Jul 2005
doi: 10.1242/dev.01934

This Article
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
dev.01934v1
132/16/3609    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Zelina, P.
Right arrow Articles by Pollerberg, G. E.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Zelina, P.
Right arrow Articles by Pollerberg, G. E.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?

Research article

The cell adhesion molecule NrCAM is crucial for growth cone behaviour and pathfinding of retinal ganglion cell axons


Pavol Zelina, Hasan X. Avci, Karsten Thelen, and G. Elisabeth Pollerberg*
* Author for correspondence (e-mail: g.e.pollerberg{at}urz.uni-heidelberg.de)

We investigated the role of the cell adhesion molecule NrCAM for axonal growth and pathfinding in the developing retina. Analysis of the distribution pattern of NrCAM in chick embryo retina sections and flat-mounts shows its presence during extension of retinal ganglion cell (RGC) axons; NrCAM is selectively present on RGC axons and is absent from the soma. Single cell cultures show an enrichment of NrCAM in the distal axon and growth cone. When offered as a substrate in addition to Laminin, NrCAM promotes RGC axon extension and the formation of growth cone protrusions. In substrate stripe assays, mimicking the NrCAM-displaying optic fibre layer and the Laminin-rich basal lamina, RGC axons preferentially grow on NrCAM lanes. The three-dimensional analysis of RGC growth cones in retina flat-mounts reveals that they are enlarged and form more protrusions extending away from the correct pathway under conditions of NrCAM-inhibition. Time-lapse analyses show that these growth cones pause longer to explore their environment, proceed for shorter time spans, and retract more often than under control conditions; in addition, they often deviate from the correct pathway towards the optic fissure. Inhibition of NrCAM in organ-cultured intact eyes causes RGC axons to misroute at the optic fissure; instead of diving into the optic nerve head, these axons cross onto the opposite side of the retina. Our results demonstrate a crucial role for NrCAM in the navigation of RGC axons in the developing retina towards the optic fissure, and also for pathfinding into the optic nerve.
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter    What's this?


This article has been cited by other articles:


Home page
 Mol. Biol. CellHome page
F. Fuentes and C. O. Arregui
Microtubule and Cell Contact Dependency of ER-bound PTP1B Localization in Growth Cones
Mol. Biol. Cell, March 15, 2009; 20(6): 1878 - 1889.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
K. Thelen, T. Georg, S. Bertuch, P. Zelina, and G. E. Pollerberg
Ubiquitination and Endocytosis of Cell Adhesion Molecule DM-GRASP Regulate Its Cell Surface Presence and Affect Its Role for Axon Navigation
J. Biol. Chem., November 21, 2008; 283(47): 32792 - 32801.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
A. J. Pittman, M.-Y. Law, and C.-B. Chien
Pathfinding in a large vertebrate axon tract: isotypic interactions guide retinotectal axons at multiple choice points
Development, September 1, 2008; 135(17): 2865 - 2871.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
E. Chatzopoulou, A. Miguez, M. Savvaki, G. Levasseur, A. Muzerelle, M.-P. Muriel, O. Goureau, K. Watanabe, L. Goutebroze, P. Gaspar, et al.
Structural Requirement of TAG-1 for Retinal Ganglion Cell Axons and Myelin in the Mouse Optic Nerve
J. Neurosci., July 23, 2008; 28(30): 7624 - 7636.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
M. P. Koester, O. Muller, and G. E. Pollerberg
Adenomatous Polyposis Coli Is Differentially Distributed in Growth Cones and Modulates Their Steering
J. Neurosci., November 14, 2007; 27(46): 12590 - 12600.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
T. Boiko, M. Vakulenko, H. Ewers, C. C. Yap, C. Norden, and B. Winckler
Ankyrin-Dependent and -Independent Mechanisms Orchestrate Axonal Compartmentalization of L1 Family Members Neurofascin and L1/Neuron-Glia Cell Adhesion Molecule
J. Neurosci., January 17, 2007; 27(3): 590 - 603.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
H. Thompson, O. Camand, D. Barker, and L. Erskine
Slit Proteins Regulate Distinct Aspects of Retinal Ganglion Cell Axon Guidance within Dorsal and Ventral Retina
J. Neurosci., August 2, 2006; 26(31): 8082 - 8091.
[Abstract] [Full Text] [PDF]


© The Company of Biologists Ltd 2005