The fully linked HTML version of this article has now been published.
Development ePress online publication date 1 Mar 2006
doi: 10.1242/dev.02312
Research article
Dorsally derived netrin 1 provides an inhibitory cue and elaborates the 'waiting period' for primary sensory axons in the developing spinal cord
Keisuke Watanabe,
Nobuaki Tamamaki,
Takahiro Furuta,
Susan L. Ackerman,
Kazuhiro Ikenaka,
and
Katsuhiko Ono*
* Author for correspondence (e-mail: katsono{at}nips.ac.jp)
Dorsal root ganglion (DRG) neurons extend axons to specific targets in the gray matter of the spinal cord. During development, DRG axons grow into the dorsolateral margin of the spinal cord and projection into the dorsal mantle layer occurs after a 'waiting period' of a few days. Netrin 1 is a long-range diffusible factor expressed in the ventral midline of the developing neural tube, and has chemoattractive and chemorepulsive effects on growing axons. Netrin 1 is also expressed in the dorsal spinal cord. However, the roles of dorsally derived netrin 1 remain totally unknown. Here, we show that dorsal netrin 1 controls the correct guidance of primary sensory axons. During the waiting period, netrin 1 is transiently expressed or upregulated in the dorsal spinal cord, and the absence of netrin 1 results in the aberrant projection of sensory axons, including both cutaneous and proprioceptive afferents, into the dorsal mantle layer. Netrin 1 derived from the dorsal spinal cord, but not the floor plate, is involved in the correct projection of DRG axons. Furthermore, netrin 1 suppresses axon outgrowth from DRG in vitro. Unc5crcm mutant shows abnormal invasion of DRG axons as observed in netrin 1 mutants. These results are the first direct evidence that netrin 1 in the dorsal spinal cord acts as an inhibitory cue for primary sensory axons and is a crucial signal for the formation of sensory afferent neural networks.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati 
Twitter What's this?
This article has been cited by other articles:
|
|
|
|
 
R. Muramatsu, S. Nakahara, J. Ichikawa, K. Watanabe, N. Matsuki, and R. Koyama
The ratio of 'deleted in colorectal cancer' to 'uncoordinated-5A' netrin-1 receptors on the growth cone regulates mossy fibre directionality
Brain,
October 25, 2009;
(2009)
awp266v1.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. B. Treloar, A. Ray, L. A. Dinglasan, M. Schachner, and C. A. Greer
Tenascin-C Is an Inhibitory Boundary Molecule in the Developing Olfactory Bulb
J. Neurosci.,
July 29, 2009;
29(30):
9405 - 9416.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Masuda, K. Watanabe, C. Sakuma, K. Ikenaka, K. Ono, and H. Yaginuma
Netrin-1 Acts as a Repulsive Guidance Cue for Sensory Axonal Projections toward the Spinal Cord
J. Neurosci.,
October 8, 2008;
28(41):
10380 - 10385.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. W. Moore, K. Lai Wing Sun, F. Xie, P. A. Barker, M. Conti, and T. E. Kennedy
Soluble Adenylyl Cyclase Is Not Required for Axon Guidance to Netrin-1
J. Neurosci.,
April 9, 2008;
28(15):
3920 - 3924.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. Schmidt, A. Stonkute, R. Juttner, S. Schaffer, J. Buttgereit, R. Feil, F. Hofmann, and F. G. Rathjen
The receptor guanylyl cyclase Npr2 is essential for sensory axon bifurcation within the spinal cord
J. Cell Biol.,
October 22, 2007;
179(2):
331 - 340.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Conrad, H. Genth, F. Hofmann, I. Just, and T. Skutella
Neogenin-RGMa Signaling at the Growth Cone Is Bone Morphogenetic Protein-independent and Involves RhoA, ROCK, and PKC
J. Biol. Chem.,
June 1, 2007;
282(22):
16423 - 16433.
[Abstract]
[Full Text]
[PDF]
|
|
|
© The Company of Biologists Ltd 2006
