spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search    

The fully linked HTML version of this article has now been published.
Development ePress online publication date 19 Jul 2006
doi: 10.1242/dev.02498

This Article
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
dev.02498v1
133/16/3159    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Sridhar, V. V.
Right arrow Articles by Liu, Z.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Sridhar, V. V.
Right arrow Articles by Liu, Z.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?

Research article

APETALA1 and SEPALLATA3 interact with SEUSS to mediate transcription repression during flower development


Vaniyambadi V. Sridhar, Anandkumar Surendrarao, and Zhongchi Liu*
* Author for correspondence (e-mail: zliu{at}umd.edu)

The transcriptional repression of key regulatory genes is crucial for plant and animal development. Previously, we identified and isolated two Arabidopsis transcription co-repressors LEUNIG (LUG) and SEUSS (SEU) that function together in a putative co-repressor complex to prevent ectopic AGAMOUS (AG) transcription in flowers. Because neither LUG nor SEU possesses a recognizable DNA-binding motif, how they are tethered to specific target promoters remains unknown. Using the yeast two-hybrid assay and a co-immunoprecipitation assay, we showed that APETALA1 (AP1) and SEPALLATA3 (SEP3), both MADS box DNA-binding proteins, interacted with SEU. The AP1-SEU protein-protein interaction was supported by synergistic genetic interactions between ap1 and seu mutations. The role of SEU proteins in bridging the interaction between AP1/SEP3 and LUG to repress target gene transcription was further demonstrated in yeast and plant cells, providing important mechanistic insights into co-repressor function in plants. Furthermore, a direct in vivo association of SEU proteins with the AG cis-regulatory element was shown by chromatin immunoprecipitation. Accordingly, a reporter gene driven by the AG cis-element was able to respond to AP1- and SEP3-mediated transcriptional repression in a transient plant cell system when supplied with SEU and LUG. These results suggest that AP1 and SEP3 may serve as the DNA-binding partners of SEU/LUG. Our demonstration of the direct physical interaction between SEU and the C-terminal domain of SEP3 and AP1 suggests that AP1 and SEP3 MADS box proteins may interact with positive, as well as negative, regulatory proteins via their C-terminal domains, to either stimulate or repress their regulatory targets.
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter    What's this?


This article has been cited by other articles:


Home page
 Plant Physiol.Home page
X. Liu, J. Huang, S. Parameswaran, T. Ito, B. Seubert, M. Auer, A. Rymaszewski, G. Jia, H. A. Owen, and D. Zhao
The SPOROCYTELESS/NOZZLE Gene Is Involved in Controlling Stamen Identity in Arabidopsis
Plant Physiology, November 1, 2009; 151(3): 1401 - 1411.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
M. J. Kim, R. Shin, and D. P. Schachtman
A Nuclear Factor Regulates Abscisic Acid Responses in Arabidopsis
Plant Physiology, November 1, 2009; 151(3): 1433 - 1445.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
C. Liu, Z. Thong, and H. Yu
Coming into bloom: the specification of floral meristems
Development, October 15, 2009; 136(20): 3379 - 3391.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
V. F. Irish
Evolution of petal identity
J. Exp. Bot., July 1, 2009; 60(9): 2517 - 2527.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
A. T. Maier, S. Stehling-Sun, H. Wollmann, M. Demar, R. L. Hong, S. Haubeiss, D. Weigel, and J. U. Lohmann
Dual roles of the bZIP transcription factor PERIANTHIA in the control of floral architecture and homeotic gene expression
Development, May 15, 2009; 136(10): 1613 - 1620.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
K. Shibuya, S. Fukushima, and H. Takatsuji
RNA-directed DNA methylation induces transcriptional activation in plants
PNAS, February 3, 2009; 106(5): 1660 - 1665.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
M. Robertson, C. A. Helliwell, and E. S. Dennis
Post-Translational Modifications of the Endogenous and Transgenic FLC Protein in Arabidopsis thaliana
Plant Cell Physiol., December 1, 2008; 49(12): 1859 - 1866.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
J. Sitaraman, M. Bui, and Z. Liu
LEUNIG_HOMOLOG and LEUNIG Perform Partially Redundant Functions during Arabidopsis Embryo and Floral Development
Plant Physiology, June 1, 2008; 147(2): 672 - 681.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
C. D. Mara and V. F. Irish
Two GATA Transcription Factors Are Downstream Effectors of Floral Homeotic Gene Action in Arabidopsis
Plant Physiology, June 1, 2008; 147(2): 707 - 718.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
S. Azhakanandam, S. Nole-Wilson, F. Bao, and R. G. Franks
SEUSS and AINTEGUMENTA Mediate Patterning and Ovule Initiation during Gynoecium Medial Domain Development
Plant Physiology, March 1, 2008; 146(3): 1165 - 1181.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
R. Tapia-Lopez, B. Garcia-Ponce, J. G. Dubrovsky, A. Garay-Arroyo, R. V. Perez-Ruiz, S.-H. Kim, F. Acevedo, S. Pelaz, and E. R. Alvarez-Buylla
An AGAMOUS-Related MADS-Box Gene, XAL1 (AGL12), Regulates Root Meristem Cell Proliferation and Flowering Transition in Arabidopsis
Plant Physiology, March 1, 2008; 146(3): 1182 - 1192.
[Abstract] [Full Text] [PDF]

Home page
 ANN BOT (LOND)Home page
G. Theissen and R. Melzer
Molecular Mechanisms Underlying Origin and Diversification of the Angiosperm Flower
Ann. Bot., September 1, 2007; 100(3): 603 - 619.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
D. Gonzalez, A. J. Bowen, T. S. Carroll, and R. S. Conlan
The Transcription Corepressor LEUNIG Interacts with the Histone Deacetylase HDA19 and Mediator Components MED14 (SWP) and CDK8 (HEN3) To Repress Transcription
Mol. Cell. Biol., August 1, 2007; 27(15): 5306 - 5315.
[Abstract] [Full Text] [PDF]

Home page
 ANN BOT (LOND)Home page
D. E. Soltis, A. S. Chanderbali, S. Kim, M. Buzgo, and P. S. Soltis
The ABC Model and its Applicability to Basal Angiosperms
Ann. Bot., August 1, 2007; 100(2): 155 - 163.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
S. R. Yadav, K. Prasad, and U. Vijayraghavan
Divergent Regulatory OsMADS2 Functions Control Size, Shape and Differentiation of the Highly Derived Rice Floret Second-Whorl Organ
Genetics, May 1, 2007; 176(1): 283 - 294.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
Flowering Newsletter bibliography for 2006
J. Exp. Bot., April 20, 2007; (2007) erm028v2.
[Full Text] [PDF]

Home page
 Plant CellHome page
S. Bezhani, C. Winter, S. Hershman, J. D. Wagner, J. F. Kennedy, C. S. Kwon, J. Pfluger, Y. Su, and D. Wagner
Unique, Shared, and Redundant Roles for the Arabidopsis SWI/SNF Chromatin Remodeling ATPases BRAHMA and SPLAYED
PLANT CELL, February 1, 2007; 19(2): 403 - 416.
[Abstract] [Full Text] [PDF]


© The Company of Biologists Ltd 2006