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Development ePress online publication date 21 Jan 2009
doi: 10.1242/dev.025569

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136/5/729    most recent
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Research article

The non-methylated DNA-binding function of Kaiso is not required in early Xenopus laevis development


Alexey Ruzov, Ekaterina Savitskaya, Jamie A. Hackett, James P. Reddington, Anna Prokhortchouk, Monika J. Madej, Nikolai Chekanov, Minghui Li, Donncha S. Dunican, Egor Prokhortchouk, Sari Pennings, and Richard R. Meehan*
* Author for correspondence (e-mail: r.meehan{at}hgu.mrc.ac.uk)

Mammalian forms of the transcription repressor, Kaiso, can reportedly bind methylated DNA and non-methylated CTGCNA motifs. Here we compare the DNA-binding properties of Kaiso from frog, fish and chicken and demonstrate that only the methyl-CpG-binding function of Kaiso is evolutionarily conserved. We present several independent experimental lines of evidence that the phenotypic abnormalities associated with xKaiso-depleted Xenopus laevis embryos are independent of the putative CTGCNA-dependent DNA-binding function of xKaiso. Our analysis suggests that xKaiso does not play a role in the regulation of either xWnt11 or Siamois, key signalling molecules in the Wnt pathway during X. laevis gastrulation. The major phenotypic defects associated with xKaiso depletion are premature transcription activation before the mid-blastula transition and concomitant activation of a p53-dependent cell-death pathway.
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