Subject collection: Stem cells & regeneration
- Generation of thalamic neurons from mouse embryonic stem cells
Highlighted article: Using 3D self-organizing culture techniques, thalamic progenitors can be induced from mouse embryonic stem cells, and show appropriate gene expression and axonal projection patterns.
- Enthesis regeneration: a role for Gli1+ progenitor cells
Summary: Tendon enthesis progenitor cells are capable of healing enthesis injuries in immature mice, in a hedgehog pathway-dependent manner. This capacity is lost in mature animals.
- Tracking the embryonic stem cell transition from ground state pluripotency
Summary: Extinction of mouse embryonic stem cell identity upon differentiation immediately follows collapse of the naïve pluripotency transcription factor circuitry and precedes upregulation of lineage-specific factors.
- Direct conversion of root primordium into shoot meristem relies on timing of stem cell niche development
- Diversity of fate outcomes in cell pairs under lateral inhibition
Highlighted article: Notch/Delta signalling in Drosophila intestinal stem cells can result in asymmetric or symmetric outcomes, depending on the activation threshold - which depends on cell contact area.
- Transient translational quiescence in primordial germ cells
- Shh promotes direct interactions between epidermal cells and osteoblast progenitors to shape regenerated zebrafish bone
- Intestinal epithelial organoids fuse to form self-organizing tubes in floating collagen gels
Summary: Culturing conventional mouse intestinal organoid cultures in contracting collagen gel induces the formation of macroscopic tubes, with all major cell types and crypt- and villus-like domains.
- Nutritional modulation of mouse and human liver bud growth through a branched-chain amino acid metabolism
Summary: Expansion of human embryonic liver progenitors in organoid culture is promoted by branched-chain amino acid metabolism, while dietary restriction in pregnant mice impairs embryonic liver bud growth.
- Self-organisation after embryonic kidney dissociation is driven via selective adhesion of ureteric epithelial cells
Summary: Time-lapse imaging, high-resolution confocal analyses and mathematical modelling demonstrate how component cells of a developing tissue can reform complex multicellular structures, even after dissociation.