Morpholinos for splice modificatio

Morpholinos for splice modification



Prospective midbrain and cerebellum formation are coordinated by FGF ligands produced by the isthmic organizer. Previous studies have suggested that midbrain and cerebellum development require different levels of FGF signaling. However, little is known about the extent to which specific regions within these two parts of the brain differ in their requirement for FGF signaling during embryogenesis. Here, we have explored the effects of inhibiting FGF signaling within the embryonic mouse midbrain (mesencephalon) and cerebellum (rhombomere 1) by misexpressing sprouty2 (Spry2) from an early stage. We show that such Spry2 misexpression moderately reduces FGF signaling, and that this reduction causes cell death in the anterior mesencephalon, the region furthest from the source of FGF ligands. Interestingly, the remaining mesencephalon cells develop into anterior midbrain, indicating that a low level of FGF signaling is sufficient to promote only anterior midbrain development. Spry2 misexpression also affects development of the vermis, the part of the cerebellum that spans the midline. We found that, whereas misexpression of Spry2 alone caused loss of the anterior vermis, reducing FGF signaling further, by decreasing Fgf8 gene dose, resulted in loss of the entire vermis. Our data suggest that cell death is not responsible for vermis loss, but rather that it fails to develop because reducing FGF signaling perturbs the balance between vermis and roof plate development in rhombomere 1. We suggest a molecular explanation for this phenomenon by providing evidence that FGF signaling functions to inhibit the BMP signaling that promotes roof plate development.


Remarkable progress has been made in understanding how the development of anatomically and functionally distinct subdivisions of the vertebrate brain is controlled. Midbrain and cerebellum formation has been particularly well studied (Raible and Brand, 2004; Zervas et al., 2005). The midbrain develops from the mesencephalon (mes) (see Fig. 1D). The dorsal mes gives rise to the tectum, which comprises the superior colliculus (SC) anteriorly and inferior colliculus (IC) posteriorly, where visual and auditory stimuli are processed, respectively. The cerebellum develops from dorsal rhombomere 1 (r1): the anterior-most segment of the hindbrain (see Fig. 1D). In mouse, the cerebellum consists of a medial vermis, flanked laterally by a pair of hemispheres (see Fig. 1E). The cerebellum, where motor activity is coordinated, undergoes dramatic changes after birth that transform it into a highly foliated structure (Sillitoe and Joyner, 2007). The molecular mechanisms that subdivide the tectum and cerebellum into subregions are poorly understood.

At early stages, signals that pattern both mes and r1 are produced by a signaling center, the isthmic organizer (IsO), at the mes/r1 boundary (Echevarria et al., 2003; Nakamura et al., 2005; Partanen, 2007), thereby ensuring coordination of midbrain and cerebellum development. One key component of the IsO signal is FGF8 (Crossley et al., 1996), a member of the fibroblast growth factor (FGF) family (Itoh and Ornitz, 2004). In mouse, Fgf8 is expressed in r1 from early neural plate [embryonic day (E) 8.25] through mid-gestation (E12.5) stages. By E10.0, Fgf8 expression is localized in r1, just posterior to the mes/r1 boundary, in a circular domain that is interrupted by Fgf8-negative regions at the dorsal and ventral midlines (roof and floor plates, respectively) (Crossley and Martin, 1995). When Fgf8 is inactivated in the early neural plate, all mes and r1 cells die between E8.5 and E10. However, when Fgf8 expression is only moderately reduced, the anterior midbrain appears normal, but posterior midbrain, isthmus and vermis are lost (Chi et al., 2003). The reason for such tissue loss in Fgf8 hypomorphs is unknown.

FGF8 regulates the expression of Fgf17 (Chi et al., 2003), which is detected in a broad domain encompassing both prospective posterior midbrain and cerebellum (Xu et al., 2000). In Fgf17-null mice, part of the IC and anterior vermis are absent, but the remaining midbrain and cerebellum appear normal. The extent of vermis loss is increased in these mutants by removing one copy of Fgf8, suggesting that the two FGF family members cooperate to control cerebellum development (Xu et al., 2000).

The level of FGF signaling can affect cell fate during mes/r1 development. Ectopic expression of an Fgf8 splice variant, Fgf8b, which encodes an FGF8 isoform with high affinity for FGF receptors (Olsen et al., 2006), transforms mouse mes cells to a cerebellar fate (Liu et al., 1999). By contrast, ectopic expression of Fgf8a, which encodes an FGF8 isoform with much lower affinity for FGF receptors (Olsen et al., 2006; Zhang et al., 2006), expands the mes and transforms posterior forebrain (diencephalon) progenitors to a midbrain fate (Lee et al., 1997; Liu et al., 1999). Importantly, when Fgf8b is ectopically expressed at low rather than high level in chicken embryos, the results are similar to those obtained with Fgf8a or Fgf17. Together, these data indicate that specification of midbrain and cerebellum require different levels of FGF signaling (Sato et al., 2001; Liu et al., 2003), and highlight the importance of controlling FGF signaling during mes/r1 development.

Fig. 1.

Spry2-GOF, a conditional Spry2 gain-of-function allele, and its expression following recombination by En1Cre. (A) Schematic diagram illustrating the Spry2-GOF transgene. The CAGG promoter drives expression of the β-Geo gene, which is followed by a triple polyadenylation sequence (3× pA). Cre-mediated recombination deletesβ -Geo, and mouse Spry2 and human placental alkaline phosphatase (PLAP) cDNAs are expressed as a bicistronic mRNA containing an internal ribosome entry site (IRES) that directs translation of PLAP. (B) Assays in whole mount for β-Geo expression (β-GAL activity) in embryos hemizygous for Spry2-GOF. (C-K) Analysis of expression of the recombined Spry2-GOF transgene in En1Cre/+;Spry2-GOF (mes/r1-S2GOF) mutants. (C-E) Assays for PLAP activity in embryos and postnatal brain at the stages indicated. Blue staining identifies cells in which recombination of the transgene occurred. The arrow in D indicates two ventrolateral stripes in the spinal cord. (F-K) RNA in situ hybridization assays in whole mount at the stages indicated, using a mouse Spry2 probe. The broken white lines outline the left side of mes/r1 in the control embryos, where the probe detects endogenous Spry2 expression. In mes/r1-S2GOF embryos, the Spry2 RNA detected represents the sum of expression from the endogenous Spry2 gene and the recombined transgene. Regions in which there is ectopic Spry2 expression in mes and in r1 are indicated by broken red lines (I,K) and by a red arrow (K), respectively. AER, apical ectodermal ridge of the limb bud; BA1, first branchial arch; CbV, cerebellar vermis; CbH, cerebellar hemispheres; Mb, midbrain; mes; mesencephalon; r1, rhombomere 1; so, somites.

Such control can be achieved intracellularly by sprouty proteins (Hacohen et al., 1998; Casci et al., 1999), the expression of which is induced by signaling through FGF receptors (FGFRs) and other receptor tyrosine kinases (RTKs) (Mason et al., 2006). There are four sprouty genes in mouse and human. Spry1 and Spry2 are strongly expressed in mes/r1 from early neural plate stages, in cells near the IsO (Minowada et al., 1999). In zebrafish (Furthauer et al., 2001) and chicken (Suzuki-Hirano et al., 2005) embryos, increasing sprouty gene expression above normal levels can antagonize FGF activity and interfere with mes/r1 development.

Recent studies have demonstrated that the roof plate in r1 is another important source of signals, including BMP ligands, for cerebellar development (Chizhikov et al., 2006; Machold et al., 2007). Roof plate development itself is controlled by Lmx1a (Millonig et al., 2000), a gene positively regulated by BMP signaling (Chizhikov and Millen, 2004a; Chizhikov et al., 2006). It is not known whether signals emanating from the IsO affect the development or function of the cerebellar roof plate or vice versa.

Here, we have employed a mouse line carrying a conditional Spry2 gain-of-function transgene in conjunction with an Fgf8null allele to produce the equivalent of an FGF loss-of-function allelic series specifically in mes/r1. By studying the phenotypic effects of these genetic manipulations, we have uncovered potential mechanism(s) by which FGF signaling differentially regulates the development of specific regions within the midbrain and cerebellum.



All mutant alleles were maintained on a mixed genetic background. Genotypes were determined by PCR assays using embryonic yolk sac or tail DNA as a template. The Spry2-GOF allele (Calmont et al., 2006), which was produced essentially as described by Lu et al. (Lu et al., 2006), was detected using primers that amplify human PLAP (ALPP). Spry2 gain-of-function homozygotes were identified using a quantitative PCR assay for PLAP sequences (primers and conditions available upon request). Other alleles were detected as previously described: En1Cre and Fgf8null (Chi et al., 2003); Fgf8neo (Sun et al., 1999); and Fgf17 (Xu et al., 2000).

Histological analysis and assays for cell death and gene expression

Noon of the day when a vaginal plug was detected was considered E0.5. Embryos were staged more precisely by counting somites posterior to the forelimb bud and scoring the first one counted as somite 13. Histological analysis of late gestation and postnatal brains, and immunolocalization in brain tissue were performed as previously described (Chi et al., 2003).

Assays for cell death were performed in whole mount by staining with LysoTrackerT (Molecular Probes L-7528), as previously described by Grieshammer et al. (Grieshammer et al., 2005). We confirmed that LysoTrackerT staining gave results on En1Cre/+;Fgf8flox/- (referred to here as mes/r1-Fgf8-KO) embryos similar to those we previously obtained using Nile Blue Sulfate staining and TUNEL assays to detect cell death (Chi et al., 2003).

For gene expression analysis, embryos were collected in cold PBS, fixed in 4% paraformaldehyde and stored in 70% ethanol at -20°C. Some embryos were embedded in paraffin and sectioned at 7 μm. Standard protocols were employed for RNA in situ hybridization on sections and in whole mount, and for assaying β-GAL and PLAP activity in whole mount.


Cre-mediated recombination of a conditional Spry2 gain-of-function transgene in midbrain and cerebellum progenitors

To reduce FGF signaling in mes/r1, we employed a mouse line carrying a conditional Spry2 gain-of-function allele, Spry2-GOF (Calmont et al., 2006). In these mice, transgene-expressing cells produce β-GEO, a fusion protein with both neomycin-resistance and β-galactosidase (β-gal) activities (Friedrich and Soriano, 1991) (Fig. 1A). β-Gal assays demonstrated that Spry2-GOF is expressed in most cells of the embryo from E7.5 to at least E11.5 (Fig. 1B, and data not shown). Cells in which the transgene has undergone Cre-mediated recombination express a bicistronic mRNA containing both Spry2- and human placental alkaline phosphatase (PLAP)-coding sequences (Fig. 1A). PLAP protein thus functions as a convenient reporter for expression of the recombined transgene.

To obtain mice in which the transgene was recombined in mes/r1, we crossed animals carrying Spry2-GOF and En1Cre, an En1 allele with cre inserted in the first exon (Kimmel et al., 2000). In their En1Cre/+;Spry2-GOF offspring, we detected PLAP activity only in the domains in which En1Cre is known to function (Li et al., 2002; Chi et al., 2003). Thus, in the developing brain, no PLAP activity was detected prior to E8.25 (not shown), but strong PLAP activity was observed throughout mes/r1 from E8.75 (Fig. 1C). Subsequently, because the CAGG promoter remains active in most embryo and adult cells, PLAP activity was detected throughout the mes and r1 at E10.75 (Fig. 1D), and the midbrain and cerebellum on postnatal day (P) 22 (Fig. 1E). In addition, by E10.75, PLAP activity was detected in the first branchial arch (Fig. 1D), presumably in mes/r1-derived neural crest cells that continue to express the recombined Spry2-GOF transgene under the control of the CAGG promoter. PLAP activity was also detected in other domains in which En1 is normally expressed (Kimmel et al., 2000) (Fig. 1D). No PLAP activity was detected in control littermates (wild type or mutants carrying only En1Cre/+ or only Spry2-GOF; not shown). En1Cre/+;Spry2-GOF mutants will hereafter be referred to as mes/r1-S2GOF embryos, to indicate that the recombined transgene was expressed in mes/r1.

To assess Spry2 expression directly from the recombined transgene, we performed a whole-mount RNA in situ hybridization analysis on mes/r1-S2GOF mutants and control littermates. Consistent with previous studies (Minowada et al., 1999), in control embryos we detected Spry2 RNA at E8.75 in a domain encompassing most of mes/r1 (Fig. 1F), which subsequently became progressively more restricted, such that by E9.25 and at E10.75 it encompassed the posterior mes and r1 but not the anterior mes (Fig. 1H,J). In mes/r1-S2GOF mutants at these stages (Fig. 1C,D), the level of Spry2 RNA appeared to be only slightly increased within the normal domains of Spry2 expression (Fig. 1G,I,K). However, because transgene expression persisted in regions where Spry2 expression is normally downregulated, we detected ectopic Spry2 expression in anterior mes and posterior r1 at later stages (E9.25 and E10.75; Fig. 1I,K). In situ hybridization analysis on sections of mes/r1-S2GOF mutants at 42 somites demonstrated that Spry2 RNA was distributed uniformly within the ectopic expression domain (not shown). Together, our data suggest that in mes/r1-S2GOF embryos, the level of Spry2 RNA is slightly elevated within its normal expression domain and is ectopically expressed throughout the remainder of the developing midbrain and cerebellum from at least E8.75.

Similar effects on midbrain and cerebellum are obtained by expressing one copy of recombined Spry2-GOF in mes/r1 or by reducing Fgf17 and Fgf8 gene dose

Histological analysis of mes/r1-S2GOF mutants shortly before birth revealed the effects of expressing the recombined transgene on midbrain and cerebellum development. In midsagittal sections of E17.5 mes/r1-S2GOF mutants (n=4), the dorsal midbrain appeared normal at its anterior end, some tissue loss was observed at the posterior end of the SC, and the IC was absent (Fig. 2A,B; data not shown). Staining for calretinin confirmed the absence of the IC, which is normally calretinin-negative (Fig. 2C,D). Posterior to the midbrain, the dorsal isthmus was also absent (Fig. 2A-D). The medial cerebellum (vermis) also appeared to have lost tissue, but only at its anterior end (Fig. 2A,B, and data not shown). By contrast, basal plate derivatives in the midbrain [oculomotor nucleus (nIII), substantia nigra and ventral tegmental area (SN-VTA)], and the isthmus [trochlear nucleus (nIV)], were present, as was the r1-derived locus coeruleus, and all appeared normal (not shown).

Mes/r1-S2GOF mutants were viable, and lacked the IC and isthmus postnatally (Fig. 2G,H), demonstrating that the apparent tissue loss observed at E17.5 was not due to a developmental delay. In wild-type mice, there are strain-specific variations in vermis foliation pattern, such that some strains have three distinct lobules (I, II and III) anterior to the lobule of culmen (IV-V), and other strains have only two (indicated as I-II and III in Fig. 2G) (Inouye and Oda, 1980). In postnatal mes/r1-S2GOF mutants (n=5), lobules I-III were either reduced or absent, whereas the remaining lobules appeared essentially normal (Fig. 2H; data not shown). Thus, expressing a single copy of the recombined Spry2-GOF transgene in mes/r1 from an early developmental stage resulted in absence of the posterior dorsal midbrain (IC), isthmus and anterior vermis. There were no obvious abnormalities in these animals in other tissues that developed from cells in which the Spry2-GOF transgene underwent Cre-mediated recombination.

The phenotype of mes/r1-S2GOF mutants described above is remarkably similar to, although slightly more severe than, that observed in Fgf17-null homozygotes carrying one copy of an Fgf8 null allele (Fgf17-/-; Fgf8+/- animals) (Xu et al., 2000). At birth, the IC, isthmus and anterior tissue in the developing cerebellum appeared to be absent in such compound FGF mutant animals (Fig. 2E,F), and at 3-4 weeks after birth, only one major lobule was present anterior to lobules IV-V (Fig. 2I). These results suggest that expressing a single copy of the recombined Spry2-GOF allele results in a reduction in FGF signaling during midbrain and cerebellar development to approximately the same degree as in Fgf17-/-; Fgf8+/- mutants.

Fig. 2.

Expression of a single copy of the recombined Spry2-GOF allele in mes/r1 causes absence of the inferior colliculus and anterior vermis. (A,B,E-I) Midsagittal sections of brains of the genotypes indicated, collected at the stages denoted and stained with Cresyl Violet or Hematoxylin and Eosin (I). Anterior is towards the left, posterior is towards the right. In control embryos, the posterior midbrain and anterior hindbrain regions that are absent in the mutant embryos are demarcated by red and yellow broken lines, respectively; in the mutant embryos, the regions where the missing midbrain and anterior hindbrain tissue would normally be found are indicated by red and yellow asterisks, respectively. (C,D) Higher power view of the tissue shown in A and B, respectively, stained with an antibody against calretinin (CR). The CR-negative inferior colliculus in the control is indicated by a broken red line. In the mutant, this CR-negative region, as well as the isthmus and velum medullare are absent (arrowhead). (G-I) Comparison of vermis development in control, mes/r1-S2GOF, and Fgf17-/-; Fgf8+/- animals at ∼3 weeks of age. CP, choroid plexus; IC, inferior colliculus; SC, superior colliculus; VM, velum medullare. The lobules of the vermis are indicated by Roman numerals, as described by Inouye and Oda (Inouye and Oda, 1980).

Reducing Fgf8 gene dose in mes/r1-S2GOF mutants or expressing two copies of the recombined Spry2-GOF allele in mes/r1 results in loss of the cerebellar vermis

If expression of the recombined Spry2-GOF allele in mes/r1 does indeed function to reduce FGF signaling, one might expect that reducing it even further, for example by reducing Fgf8 gene dose, would result in a more severe phenotype. To test this prediction, we examined En1Cre/+;Spry2-GOF;Fgf8+/- (mes/r1-S2GOF;F8) mutants at E17.5. When viewed in whole mount, it was evident that they lacked the entire vermis (Fig. 3A,B). Histological analysis showed that only the anterior region of the dorsal midbrain, including the anterior-most mes-derived structure, the PPT (Lagares et al., 1994), appeared normal; tissue was missing at the posterior end of the SC; and the IC, dorsal isthmus and vermis were absent (Fig. 3C-E). Mes/r1-S2GOF;F8 mutants could be classified in two groups: group I (n=3/5) had some tissue loss at the posterior end of the SC, and basal structures including nIII, nIV and the SN-VTA were present although somewhat reduced; group II (n=2/5) had more tissue loss at the posterior end of the SC and the basal structures were absent (Fig. 3C-K). Lateral r1-derived tissue was present in both groups, as evidenced by the presence of the locus coeruleus, but it was clearly reduced in group II (Fig. 3I-K).

We next examined animals carrying En1Cre/+ and two copies of Spry2-GOF (mes/r1-S2GOF;S2GOF; n=3), which appeared similar to mes/r1-S2GOF;F8 mutants in group I (not shown). Thus, expressing a second copy of the recombined Spry2-GOF allele appeared to inhibit FGF signaling to approximately the same extent as removing one copy of the Fgf8 gene in mes/r1-S2GOF animals. The phenotype of the group I mes/r1-S2GOF;F8 and mes/r1-S2GOF;S2GOF mutants is similar to that observed in embryos homozygous for Fgf8neo (Chi et al., 2003), a hypomorphic allele of Fgf8, that has been estimated to express Fgf8 RNA at ∼40% of the level in wild-type embryos (Meyers et al., 1998). Unlike Fgf8neo/neo animals, which have numerous developmental abnormalities caused by reduced FGF8 signaling in all tissues and die at birth, several mes/r1-S2GOF;F8 and mes/r1-S2GOF;S2GOF mutants survived to adulthood. Thus, we were able to determine the postnatal consequences of the midbrain and cerebellum defects that were detected just before birth. In P21 mes/r1-S2GOF;F8 animals, a complete loss of vermis was readily observed in intact brains, whereas the cerebellar hemispheres were present, but appeared somewhat reduced (Fig. 3L,M). Section analysis at P28 revealed a hemisphere-like foliation pattern in lateral sections and further showed that the granule cell layer was of normal thickness; staining with calbindin demonstrated that the density of Purkinje cells was apparently normal, as were their axon and dendrite projections (see Fig. S1 in the supplementary material). Consistent with the absence of the vermis, the Fastigial nucleus was absent, whereas the Dentate nucleus appeared relatively unaffected (not shown). The lack of vermis, which controls posture and locomotion, presumably explains our finding that mes/r1-S2GOF;F8 mutants exhibited a widened gait and pronounced ataxia that increased in severity with age (not shown).

Cell death occurs in the anterior mesencephalon in Spry2-GOF mutants and Fgf8 hypomorphs

We have previously shown that eliminating Fgf8 function in mes/r1 causes extensive cell death between the 11 and 28 somite stages, resulting in complete absence of the midbrain, isthmus and cerebellum (Chi et al., 2003). However, the effects of only moderately reducing the level of FGF gene expression on cell survival in mes/r1 were not examined. We therefore sought to determine whether abnormal cell death could account for the tissue loss in embryos that were homozygous for Fgf8neo or that expressed the recombined Spry2-GOF transgene, by staining with LysotrackerT (see Materials and methods) at 4- to 6-hour intervals between E8.75 and E10.75 (12 to 38 somites).

Fig. 3.

Decreasing Fgf8 gene dose in mes/r1-S2GOF embryos results in absence of the vermis. (A,B) Dorsal views of brains collected from E17.5 embryos of the genotypes indicated. In the mutant brain, the region where the vermis would normally be found is indicated by a yellow asterisk. (C-E) Low-magnification views of midsagittal sections of E17.5 control and mes/r1-S2GOF;F8 mutants, stained with Cresyl Violet. (D,E) Examples of the group I (milder) and group II (more severe) brain phenotypes observed in the mutants. The broken red and yellow lines and red and yellow asterisks are explained in the legend to Fig. 2A,B,E,F. The areas outlined in C-E are shown at higher magnification in F-H, respectively. (F-H) High magnification views of basal structures. When present, nIII and nIV are circled with broken lines. (I-K) High magnification views of more lateral sagittal sections of the same brains, assayed by immunohistochemistry with an antibody against tyrosine hydroxylase, which specifically stains nuclei in the lateral posterior diencephalon, midbrain and anterior hindbrain. The broken red line indicates the boundary between diencephalon and midbrain. (L,M) Dorsal views of intact brains collected from P21 mice of the genotypes indicated, stained with ink. The broken line in L outlines the vermis, which is absent in the mutant brain. CbV, cerebellar vermis; CbH, cerebellar hemispheres; CP, choroid plexus; Di, diencephalon; IC, inferior colliculus; Is, isthmus; LoC, locus ceruleus; mlf, medial longitudinal fascicle; nIII, oculomotor nucleus; nIV, trochlear nucleus; PPT, posterior pretectal nucleus; SC, superior colliculus; SN-VTA, substantia nigra-ventral tegmental area.

We detected a relatively small domain in Fgf8neo/neo and mes/r1-S2GOF mutants in which cell death was more extensive than in controls, but only at the 15- to 16-somite and 18- to 20-somite stages, respectively. A similar domain of abnormal cell death was observed at 18- to 20-somites in mes/r1-S2GOF;F8 mutants, in which the level of FGF signaling is lower than in mes/r1-S2GOF mutants. Surprisingly, given that the anterior midbrain appears to develop normally in all these mutants (Fig. 2A,B; Fig. 3C-E) (Chi et al., 2003), the domain of abnormal cell death was localized at the anterior end of the dorsal mes (Fig. 4A-E; data not shown). To confirm the location of this abnormal cell death, we performed an in situ hybridization assay on the LysotrackerT-labeled embryos using a probe for Pax6. This gene is expressed throughout the prospective forebrain, with the posterior limit of its expression domain defining the boundary between the developing diencephalon (di) and mes. The domain of abnormal cell death, as detected by LysotrackerT staining, was indeed localized immediately posterior to the di/mes boundary (Fig. 4C,C′, and data not shown).

One explanation for the abnormal cell death in the mutants is that a certain level of FGF signaling from the IsO is required for cell survival, and that it falls below that level in the anterior mes when either Spry2 is ectopically expressed or Fgf8 expression is reduced (as in Fgf8neo/neo embryos). To investigate this hypothesis, we determined the range of FGF signaling in mes/r1 by assaying for Spry1 expression, which is induced by and thus serves as a reporter for a high level of FGF signaling (Liu et al., 2003; Olsen et al., 2006). We found that at the 18- to 20-somite stage, the distance from the isthmic constriction to the anterior limit of the Spry1 expression domain was reduced in mes/r1-S2GOF and mes/r1-S2GOF;F8 mutants compared with that in control embryos (Fig. 4F-H). These data provide evidence that the range of FGF signaling from the IsO is decreased in the mes of these mutants, supporting the hypothesis that the observed anterior cell death is a consequence of reduced FGF signaling.

Similar results were obtained in assays for En1, En2 and Efna2 expression (Fig. 4 and data not shown). At 33-35 somites, the Efna2 expression domain was smaller than normal in mes/r1-Spry2-GOF and even smaller in mes/r1-Spry2-GOF;F8 embryos (Fig. 4I-K). These data are consistent with studies showing that En1 and En2 expression is regulated by FGF signaling (Trokovic et al., 2003), and that ephrin gene expression is controlled by engrailed genes (Nakamura, 2001). In addition, as expected for mutants with reduced FGF signaling from the IsO (Zervas et al., 2005; Partanen, 2007), we found that the posterior limit of Otx2 expression was slightly less sharp in mes/r1-S2GOF;F8 mutants than in controls, and a few scattered Otx2-positive cells were detected in r1. Furthermore, Gbx2 and Wnt1 expression were significantly reduced in anterior r1 and the posterior midbrain, respectively (see Fig. S2 in the supplementary material).

Fig. 4.

Reducing FGF signaling from the isthmic organizer results in cell death in the anterior mesencephalon. (A-E) Lateral views of embryos of the genotypes indicated, collected at the somite stages denoted and labeled with LysotrackerT (LysoT), which marks regions in which dead cells are abundant. The approximate position of the isthmic constriction (Is) is indicated. (C′) The embryo shown in C was fixed after labeling with LysoT and assayed by RNA in situ hybridization for Pax6 expression. The broken yellow line indicates the posterior limit of Pax6 expression, which lies at the border between the prospective forebrain (diencephalon, Di) and midbrain (mesencephalon, Mes). In control embryos (A,D), LysoT labeling is detected where neural crest (NC) is migrating into the first branchial arch (BA1), but there is relatively little labeling in the neuroepithelium. By contrast, in the mutant embryos (B,C,E) there is extensive LysoT labeling in the mes (white arrows). (F-K) Embryos of the genotypes indicated, collected at the somite stages indicated, were assayed in whole mount by RNA in situ hybridization using a probe for Spry1 or Efna2. The extent of the expression domain from the mes/r1 boundary (red circle) towards the forebrain is indicated by a broken red arrow.

The roof plate is expanded in anterior rhombomere 1 in mes/r1-S2GOF;F8 mutants

Importantly, in the experiments described above we detected no abnormal cell death in the prospective posterior midbrain or cerebellum of mes/r1-S2GOF or mes/r1-S2GOF;F8 mutants (Fig. 4A-C; data not shown), suggesting that the loss of IC, isthmus and vermis in these mutants is not due to cell death between E8.75 and E11.5. An alternative explanation for the loss of vermis in mes/r1-S2GOF;F8 mutants was suggested by an analysis of transverse sections through anterior r1 at E11.5. In control embryos, the roof plate (dorsal midline) was characteristically thin, i.e. only two or three cell layers deep, across a mediolateral domain five to eight cell diameters in width (Fig. 5A,A′). By contrast, in mes/r1-S2GOF;F8 mutants at a comparable anteroposterior (AP) level, the roof plate was similarly thin but much wider than normal (Fig. 5B,B′). This region in the mutants appeared similar in width to the roof plate in more posterior sections of control and mutant embryos, where it comprised a single cell layer that was ∼30 cell diameters wide (Fig. 5C-D′).

We next examined gene expression patterns at earlier stages. In control embryos at E10.5, as well as in mes/r1-S2GOF embryos, the near circular Fgf8 expression domain in anterior r1 is interrupted at the dorsal midline by a small group of Fgf8-negative roof plate cells (Fig. 5E,F; data not shown). In mes/r1-S2GOF;F8 embryos, the Fgf8 expression pattern appeared almost normal up to E9.5 (28 somites; Fig. 5H,I), but by E10.5 (34 somites) the Fgf8-negative domain in dorsal r1 was much wider than normal (Fig. 5G, compare with 5E,F). In contrast to Fgf8, Bmp7 expression is a positive marker of the roof plate. In control and mes/r1-S2GOF embryos at E10.5, it was detected in a narrow domain in anterior r1 that progressively widened towards posterior r1 (Alder et al., 1999) (Fig. 5J,K). However, in mes/r1-S2GOF;F8 embryos, a wider Bmp7-positive roof plate domain was observed in anterior r1 (Fig. 5L, compare with 5J,K).

Together, these data provide strong evidence that the roof plate had expanded laterally in anterior r1 in mes/r1-S2GOF;F8 embryos. Fate-mapping studies in the mouse embryo have localized vermis progenitors to a small domain flanking the dorsal midline in the anterior-most region of r1 at E12.5, and there is evidence that at earlier stages these cells are likewise localized close to the dorsal midline (Sgaier et al., 2005). Thus, it appears that the region containing the vermis progenitors, which is normally positive for Fgf8 and negative for Bmp7 expression, has been replaced by Fgf8- negative Bmp7-positive roof plate cells in mes/r1-S2GOF;F8 embryos. These results indicate that reducing FGF signaling to the level attained in mes/r1-S2GOF;F8 embryos causes an expansion of the roof plate between the 28- and 34-somite stages, possibly at the expense of vermis progenitors.

BMP target gene expression is increased and BMP antagonist gene expression is decreased in mes/r1-S2GOF;F8 mutants

Previous studies have indicated that roof plate cells express several BMP family members in addition to Bmp7, and that BMP signaling is necessary and sufficient for roof plate development in the chick neural tube (Lee and Jessell, 1999; Chizhikov and Millen, 2004b; Chizhikov and Millen, 2004c; Liu et al., 2004). The transcription factor gene Msx1 is a downstream target of BMP signaling in the dorsal neural tube, and misexpression of Msx1 in the chick spinal cord results in expansion of the roof plate (Liu et al., 2004). Therefore, to determine whether excess BMP signaling might be responsible for the roof plate expansion we observed in mes/r1-S2GOF;F8 embryos at E10.5, we assayed for Msx1 expression as a readout for BMP signaling in dorsal r1. The domain of Msx1 expression was indeed significantly expanded mediolaterally in anterior r1 in mes/r1-S2GOF;F8 embryos as compared with control embryos at 33 somites (Fig. 5M,N). These data support the hypothesis that a decrease in FGF signaling in r1 results in an increase in BMP signaling and expansion of the roof plate.

Fig. 5.

The roof plate is expanded and Grem1 expression is reduced in mes/r1-S2GOF;F8 embryos. (A-D′) Transverse sections through r1 of a control and a mes/r1-S2GOF;F8 embryo at 48 somites (E11.75), stained with Hematoxylin and Eosin. Every third or fourth section in the series was assayed by RNA in situ hybridization with a probe for Otx2, to locate the posterior limit of the mesencephalon (not shown). The broken lines in the inset in A illustrate the approximate levels at which the anterior (A) and posterior (P) sections were cut, i.e. within 40-100 and 130-250 μm of the posterior limit of the Otx2 expression domain (indicated in blue), respectively. The sections in all panels are shown at the same magnification. The regions demarcated by broken boxes in A-D are shown at fourfold higher magnification in A′-D′, respectively. The mediolateral width of the dorsal midline domain in which the neuroepithelium is only two or three cell layers thick is indicated by red brackets (A′-D′). (E-L,O,P) RNA in situ hybridization assays in whole mount using the probes indicated. Dorsal views of embryos of the genotypes indicated, collected at the stages denoted. Anterior is towards the top, posterior towards the bottom. (E-I) The roof plate that bisects the Fgf8 expression domain is indicated by arrowheads. In control and mes/r1-S2GOF embryos, the Fgf8-negative roof plate is difficult to discern; it is considerably expanded mediolaterally in the mes/r1-S2GOF;F8 embryo (open arrowhead in G). The lateral and ventral aspect of the Fgf8 expression domain in r1 is visible in E and G on the left side, through the roof plate. (J-L) A broken red line is drawn at the same distance posterior to the isthmic constriction at the mes/r1 boundary (black arrow). Bmp7 expression marks the roof plate, and the roof plate widens closer to the isthmic constriction in the mes/r1-S2GOF;F8 embryo than in the control and mes/r1-S2GOF embryos. (M,N) Transverse sections through anterior r1 at approximately the same AP level as in A and B, hybridized with a probe for Msx1. The mediolateral extent of the Msx1 expression domain from the center of the roof plate (open circle) is indicated by a broken red arrow. (O,P) Grem1 expression is readily detected at the dorsal midline of r1 in the control embryo, but is barely detected in r1 of the mes/r1-S2GOF;F8 embryo (red asterisk).

In considering how FGF signaling in anterior r1 could function to inhibit BMP signaling, we investigated the possibility that it might induce or maintain the expression of genes that encode secreted BMP antagonists. One such gene is gremlin 1 (Grem1) (Hsu et al., 1998), which is expressed in the roof plate of anterior r1 (Pearce et al., 1999; Louvi et al., 2003). We found that in control embryos at E9.25 (22 somites), Grem1 RNA was readily detected in the roof plate and adjacent neuroepithelium (Fig. 5O, and data not shown), whereas in mes/r1-S2GOF;F8 embryos Grem1 expression was barely detected in anterior r1 (Fig. 5P). By contrast, Grem1 expression was only moderately reduced in mes/r1-S2GOF embryos (not shown). These results suggest that a normal function of FGF signaling from the IsO is to inhibit BMP signaling in dorsal r1 via a positive effect on Grem1 expression.


In this study, we produced the equivalent of an FGF loss-of-function allelic series specifically in mes/r1 and examined the effects of progressively reducing FGF signaling on midbrain and cerebellum development. By recombining one copy of a conditional Spry2 gain-of-function allele throughout mes/r1 at ∼E8.5, we obtained mes/r1-S2GOF mutants with a phenotype similar to that of Fgf17-/-;Fgf8+/- mutants (Xu et al., 2000). When we reduced Fgf8 gene dose in mes/r1-S2GOF mutants, or produced animals with two copies of the recombined Spry2-GOF allele, we obtained a more severe phenotype, which resembled that of embryos homozygous for an Fgf8 hypomorphic allele (Fgf8neo) (Chi et al., 2003). Together, these data strongly support the hypothesis that expression of the recombined Spry2-GOF allele results in an inhibition of signaling via FGF receptors rather than other RTKs during midbrain and cerebellum development. A dorsal mes/r1 phenotype similar to that in mes/r1-S2GOF;F8 embryos has also been observed following conditional inactivation of Fgfr1 in mes/r1 (Trokovic et al., 2003), suggesting that the FGF signaling that is affected in the Spry2 gain-of-function mutants is primarily relayed by FGFR1.

The results of our analysis provide genetic evidence consistent with the proposal that the processes that shape the midbrain and cerebellum require distinct levels of FGF signaling (Liu et al., 1999; Sato et al., 2001; Liu et al., 2003), and further reveal that anatomically and functionally distinct regions within the midbrain and cerebellum require different levels of FGF signaling for their development. This conclusion is consistent with the results of a recent analysis showing that when En1/En2 function, which is apparently downstream of FGF signaling from the IsO, is progressively compromised, specific functional domains of the tectum and vermis are lost in a dose-dependent manner (Sgaier et al., 2007). In addition, we present evidence that FGF signaling influences the balance between vermis and roof-plate development in anterior r1 via an effect on BMP signaling.

The effects of Spry2 gain-of-function in mes/r1 differ in mouse and chicken embryos

The phenotypes we observed in our mutant mice differ from those reported in chicken embryos, in which ectopic expression of Spry2 caused cells in r1 to express Otx2, a marker for the mes, and to develop into midbrain tissue (Suzuki-Hirano et al., 2005). We did observe that Otx2 expression was abnormal in mes/r1-S2GOF;F8 embryos, in that the boundary between Otx2-positive and Otx2- negative cells was not as sharp as in control embryos and there were a few Otx2-positive cells scattered in r1 (see Fig. S2 in the supplementary material). However, we found no evidence that r1 cells took on a midbrain fate. Instead, misexpressing Spry2 in mes/r1 resulted in defects in vermis development. Although there is evidence that the forced expression of Otx2 in r1 from E8.75 can transform anterior r1 into midbrain tissue in the mouse embryo (Broccoli et al., 1999), there is as yet no direct evidence that mouse r1 can give rise to midbrain tissue when FGF signaling is reduced. The disparity between our findings and those of Suzuki-Hirano et al. (Suzuki-Hirano et al., 2005) might be due to differences in the methods employed to obtain ectopic sprouty gene expression, i.e. sustained expression of an inherited transgene in the mouse versus transient expression of a transgene introduced by electroporation in the chicken embryo. Alternatively, it is possible that the different results reflect differences in the mechanisms that control the level of FGF signaling in mouse versus chicken brain development.

Loss of the posterior tectum caused by reducing FGF signaling can be explained by death of anterior cells and mis-specification of posterior cells as anterior tectum

A specific loss of the IC, with normal development of the SC, is a feature common to mutants in which FGF signaling is moderately reduced in mes/r1, including Fgf17-/-;Fgf8+/- embryos (Xu et al., 2000), Fgf8neo/neo embryos (Chi et al., 2003) and embryos in which Fgfr1 has been inactivated in mes/r1 (Trokovic et al., 2003), but the mechanism by which this loss occurs is not known. Death of IC progenitors is one possible explanation, as we previously showed that inactivation of Fgf8 in mes/r1 by the 10-somite stage results in apoptosis throughout the mes (Chi et al., 2003). However, cell death was not detected at E9.5 in the mesencephalon when Fgfr1 was inactivated (Trokovic et al., 2003).

Here, we show that a moderate reduction in FGF signaling does cause abnormal cell death prior to E9.25, but that the dying cells are detected only in the anterior mes. To explain this localization, we propose that there is a minimum level of FGF signaling below which cells in the mes die. In normal embryos, there is sufficient FGF signaling to sustain survival, even of cells that are furthest from the source of FGFs in the IsO (Fig. 6A). However, when FGF signaling is moderately reduced, as for example in mes/r1-S2GOF mutants, only cells close to the IsO attain the level of FGF signaling required for survival. At early stages, when the mes is relatively small, all the cells are sufficiently close to the IsO. But as the mes increases in size and the anterior-most cells in such mutants are displaced progressively further from the IsO, they reach a point at which they are too far from the IsO to attain the level of FGF signaling required for survival, and therefore they die.

The observation that cell death is restricted to the anterior mes suggests the following explanation for the loss of the IC in mutants with reduced FGF signaling in the mes: AP cell fates have not yet been determined in the mes at the stage when the cells are dying, and the remaining posterior cells are subsequently specified as SC because of the low level of FGF signaling. Consistent with this hypothesis, it has been shown that fate changes can occur in explants of E9.5 mouse midbrains (Liu et al., 1999). Presumably, if cells in the anterior mes had died at a stage after the fate of all mes cells had been determined, a normal SC would not have formed. This model further suggests that specification of IC progenitors requires a higher level of FGF signaling than specification of SC progenitors. This idea is supported by data showing that increasing FGF signaling, by inserting beads loaded with FGF protein in the anterior mes of chicken embryos, induces anterior cells to take on a posterior fate (Martinez et al., 1999; Shamim et al., 1999).

Loss of the vermis caused by reducing FGF signaling can be explained by an increase in BMP signaling and expansion of the roofplate

A loss of the entire vermis is observed when FGF signaling is reduced to a level lower than in Fgf17-/-;Fgf8+/- and mes/r1-S2GOF mutants. Our data indicate that abnormal cell death is not responsible for the loss of the vermis in such mutant embryos. Instead, the vermis may be absent because a specific minimum level of FGF signaling is required for specification and/or expansion of vermis progenitors (Liu et al., 2003), and that level is not attained in such mutants. Another possibility is based on our observation that the roof plate in r1 is abnormally expanded in mes/r1-S2GOF;F8 embryos. Although this abnormality might be secondary to a failure of vermis development, we favor the hypothesis that the converse is true, and that the observed expansion of the roof plate is the primary cause of loss of the vermis (Fig. 6B).

Fig. 6.

A model to explain the phenotypes obtained when FGF signaling is progressively reduced in mes/r1. Schematic diagrams illustrate dorsal views of mes/r1 in embryos of the genotypes indicated at the somite stages denoted. The intensity of the blue color in the triangle to the left of a diagram illustrates the level of FGF signaling. (A) The regions in which cells are dying or have already died are indicated by red stippling. (B) The regions that contain the progenitors (p) of the superior colliculus (SC), inferior colliculus (IC), cerebellar vermis (CbV) and cerebellar hemispheres (CbH) are indicated. The phenotypes observed in mutants that attain progressively lower levels of FGF signaling are illustrated. In mes/r1-S2GOF embryos, the anterior mes has been lost, and the surviving posterior cells are specified to an anterior (SC) fate. Consequently the IC does not develop. In mes/r1-S2GOF;F8 embryos, the effects on the mes are similar and, in addition, the vermis fails to develop because roof plate (RP) expansion in r1 results in a loss of CbV progenitors. (C) In the wild-type embryo, FGF signaling induces/maintains the expression of Grem1, which functions to inhibit BMP signaling and expression of the BMP effector MSX1. This pathway maintains the normal balance of vermis and roof plate development. In the mes/r1-S2GOF;F8 embryo, the reduction in the level of FGF signaling results in a severe reduction in Grem1 expression. In the absence of this antagonist, the level of BMP signaling and therefore MSX1 expression increases and an expanded roof plate develops where the vermis progenitors normally reside.

The model we advocate is based on studies showing that in the spinal cord, the roof plate forms in response to BMP signaling from the adjacent epidermal ectoderm (Lee and Jessell, 1999; Chizhikov and Millen, 2004c; Chizhikov and Millen, 2005), that overexpression of Lmx1a in mouse r1 is associated with increased BMP signaling and increased roof plate formation (Chizhikov et al., 2006), and that BMP signaling can be inhibited by FGF signaling in numerous developmental settings, including the forebrain (Storm et al., 2003) and midbrain (Alexandre et al., 2006). We propose that in r1, roof plate formation is likewise controlled by BMP signaling from the ectoderm, which in turn is inhibited by FGF signaling from the IsO. This inhibitory interaction could explain why the roof plate is normally so narrow in anterior r1, adjacent to the IsO where FGF ligands are produced, but is much wider in more posterior r1. Accordingly, when FGF signaling is sufficiently reduced, as in mes/r1-S2GOF;F8 embryos, BMP signaling increases, and the roof plate in anterior r1 becomes abnormally wide (Fig. 6C). In support of this idea, we found that in mes/r1-S2GOF;F8 embryos, BMP signaling is increased, as evidenced by an expansion of the Msx1 expression domain. Moreover, we observed that a dramatic reduction in expression of the BMP antagonist Grem1 precedes abnormal expansion of the roof plate by ∼12 hours, suggesting a molecular mechanism by which FGF signaling could exert a negative effect on BMP signaling. However, GREM1 alone is unlikely to be the sole factor responsible for mediating the proposed inhibitory effect of FGF signaling on BMP signaling and roof plate expansion in r1, because we found that roof plate development appears normal in Grem1-null embryos (not shown).

An important question is how might the expansion of the roof plate observed in mes/r1-S2GOF;F8 mutants compromise vermis development? One possibility is based on the fact that the roof plate itself expresses BMPs (Alder et al., 1999; Lee and Jessell, 1999; Alexandre et al., 2006), and an increase in the size of the cell population producing these potent signaling molecules might in turn cause the nearby vermis progenitors to slow their proliferation or differentiate prematurely, leading ultimately to absence of the vermis (Alder et al., 1999; Krizhanovsky and Ben-Arie, 2006; Machold et al., 2007). Alternatively, as misexpression of the BMP effector MSX1 results in the conversion of spinal cord neuroepithelum into roof plate (Liu et al., 2004), it is possible that the increase in Msx1 expression in mes/r1-S2GOF;F8 mutants functions to stimulate roof plate development at the expense of the vermis by converting vermis progenitors to a roof plate fate. Further studies will be needed to distinguish between these possibilities.

Supplementary material

Supplementary material for this article is available at


We thank Drs George Minowada, who produced the Spry2-GOF mouse lines and initiated the studies described here, and Benjamin Yu and Robert Hindges for helpful suggestions. We are grateful to P. Ghatpande and Monica Rodenas for excellent technical assistance. We also thank Drs Roy Sillitoe and Richard Wingate, and our laboratory colleagues for helpful comments on the manuscript. D.E. was supported by the Spanish Ministry of Health, Instituto de salud Carlos III, CIBERSAM (CB07/09/0021). This work was supported by grants from the Wellcome Trust (080470) to M.A.B. and (072111) to M.A.B. and I.M., by the Medical Research Council and a Leverhulme Trust Fellowship to I.M., by the EU research program (LSHG-CT-2004-512003 and MEIF-CT-2006-025154), the Spanish Science Program (MEC BFU2005-09085, RD06/0011/0012), the ELA Foundation Research and TV3 LA (MARATO-062232) to D.E. and S.M., and by the National Institutes of Health (R01 HD050767) to A.L.J. and (R01 CA78711) to G.R.M.


  • * These authors contributed equally to this work

    • Accepted November 30, 2007.


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