Pph13 is essential and sufficient for activation of the 3XP3 reporter. (A,B) 3XP3-RFP expression in a Pph13/+ heterozygote (left) compared with a homozygous Pph13 mutant (right). Note the complete loss of expression in the eyes and ocelli in the Pph13 mutant. (C,D) 3XP3-GFP expression in wild-type (left) and hs-Pph13 (right) Drosophila pupae. Both genotypes were subject to two heat shocks (37°C) twice a day for 40 minutes, commencing at 24 hours APF. Note that the ectopic expression of Pph13 is sufficient to drive expression of GFP outside of photoreceptor cells.