Caudal visceral mesoderm (CVM) cells migrate from posterior to anterior of the Drosophila embryo as two bilateral streams of cells to support the specification of longitudinal muscles along the midgut. To accomplish this long-distance migration, CVM cells receive input from their environment, but little is known about how this collective cell migration is regulated. In a screen we found that wunen mutants exhibit CVM cell migration defects. Wunens are lipid phosphate phosphatases known to regulate the directional migration of primordial germ cells (PGCs). PGC and CVM cell types interact while PGCs are en route to the somatic gonadal mesoderm, and previous studies have shown that CVM impacts PGC migration. In turn, we found here that CVM cells exhibit an affinity for PGCs, localizing to the position of PGCs whether mislocalized or trapped in the endoderm. In the absence of PGCs, CVM cells exhibit subtle changes, including more cohesive movement of the migrating collective, and an increased number of longitudinal muscles is found at anterior sections of the larval midgut. These data demonstrate that PGC and CVM cell migrations are interdependent and suggest that distinct migrating cell types can coordinately influence each other to promote effective cell migration during development.
The authors declare no competing or financial interests.
V.S., L.D., Y.-K.B. and A.S. designed the experiments; V.S., L.D., Y.-K.B., F.M., N.T. and J.S. conducted the experiments; V.S., L.D., F.M. and A.S. analyzed the data and wrote the paper.
This work was funded by the National Institutes of Health [R21HD073860 and R01GM104838 to A.S.]; the American Heart Association [11POST7600181 to Y.-K.B.]; and the American Cancer Society [PF-15-202-01-DDC to V.S.]. Deposited in PMC for release after 12 months.
Supplementary information available online at http://dev.biologists.org/lookup/doi/10.1242/dev.134346.supplemental
- Received December 18, 2015.
- Accepted July 29, 2016.