Table of Contents
IN THIS ISSUE
- Towards a CRISPR view of early human development: applications, limitations and ethical concerns of genome editing in human embryos
Summary: This Spotlight article discusses the use of CRISPR-based genome engineering in human embryos and the emerging themes therein.
- Defective adgra2 (gpr124) splicing and function in zebrafish ouchless mutants
Summary: ouchless phenotypes are attributed to an essential splice site mutation affecting adgra2 (gpr124) splicing and function, rather than to downregulated sorbs3 transcript levels, as previously thought.
- From stem cells to human development: a distinctly human perspective on early embryology, cellular differentiation and translational research
Summary: This Meeting Review summarises the major themes and results presented at the second 'From Stem Cells to Human Development' meeting, held in October 2016.
- Understanding development and stem cells using single cell-based analyses of gene expression
Summary: This Review discusses how single cell RNA sequencing has been used to study developmental and stem cell biology, providing insights into cell type diversity and developmental dynamics.
- Tfap2 and Sox1/2/3 cooperatively specify ectodermal fates in ascidian embryos
Summary: Epidermal fate is autonomously determined by a gene circuit beginning with Sox1/2/3 and Tfap2-r.b in ascidian embryos.
- Differential temporal control of Foxa.a and Zic-r.b specifies brain versus notochord fate in the ascidian embryo
Summary: The temporally non-overlapping expression of Foxa.a and Zic-r.b in the brain lineage is regulated by three repressors: Prdm1-r.a, Prdm1-r.b and Hes.a.
- Wt1 directs the lineage specification of sertoli and granulosa cells by repressing Sf1 expression
- A set of simple cell processes is sufficient to model spiral cleavage
Highlighted article: Computational modelling reveals that Sachs' rule, animal-vegetal polarization of cell division, cortical rotation and adhesion are the main contributing variables to spiral cleavage.
- Rewiring of embryonic glucose metabolism via suppression of PFK-1 and aldolase during mouse chorioallantoic branching
- Atypical chemokine receptor ACKR2 controls branching morphogenesis in the developing mammary gland
Summary: Ackr2−/− mice display precocious mammary gland development as a result of impaired chemokine scavenging and increased macrophage recruitment to the mammary gland.
- PDGFRα controls the balance of stromal and adipogenic cells during adipose tissue organogenesis
Summary: Analysis of PDGFRα gain-of-function mutant mice demonstrates a role in balancing the expansion of fibroblast and adipocyte lineages before birth.
- Remodeling of adhesion and modulation of mechanical tensile forces during apoptosis in Drosophila epithelium
Summary: The transient release of tension upon a reduction in the levels of adherens junctions components contributes to apoptotic cell extrusion in Drosophila epithelium.
- Conserved and novel functions of programmed cellular senescence during vertebrate development
Summary: Cellular senescence is an intrinsic part of the developmental programme in amphibians and has a conserved role in vertebrate organogenesis.
- Interactions between mural cells and endothelial cells stabilize the developing zebrafish dorsal aorta
Summary: Vascular smooth muscle cells are required to help maintain the vascular basement membrane and promote vessel stabilization during development.
- Translation repression by maternal RNA binding protein Zar1 is essential for early oogenesis in zebrafish
Summary: Zar1 RNA binding protein represses zona pellucida RNA translation during early oogenesis. zar1 deletion mutants show ER stress and apoptosis in oocytes and result in an all-male phenotype.
- The Drosophila Hox gene Ultrabithorax acts in both muscles and motoneurons to orchestrate formation of specific neuromuscular connections
Summary: Ultrabithorax controls correct innervation of ventrolateral muscles by coordinating Wnt4 ligand expression in muscles with the signalling pathway response in motoneurons.
- Modulation of apical constriction by Wnt signaling is required for lung epithelial shape transition
Summary: Epithelial morphogenesis during the transition from the early to late stage of lung development is coordinated by the activity of Wnt signaling.
- MS23, a master basic helix-loop-helix factor, regulates the specification and development of the tapetum in maize
Summary: A cascade of basic helix-loop-helix transcription factors guides tapetal cell development in maize anthers, using proteins conserved in Arabidopsis and rice but deployed with distinctive timing.
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From Journal of Cell Science