In recent years, genome-wide profiling approaches have begun to uncover the molecular programs that drive developmental processes. In particular, technical advances that enable genome-wide profiling of thousands of individual cells have provided the tantalizing prospect of cataloging cell type diversity and developmental dynamics in a quantitative and comprehensive manner. Here, we review how single-cell RNA sequencing has provided key insights into mammalian developmental and stem cell biology, emphasizing the analytical approaches that are specific to studying gene expression in single cells.
The authors declare no competing or financial interests.
The authors’ research is funded by the National Institutes of Health (National Institute of Diabetes and Digestive and Kidney Diseases) (K01DK096013). Deposited in PMC for release after 12 months.