Table 2.

Dauer-like larva formation of sdf-9 single mutants on NGM plates containing various concentrations of cholesterol

Dauer-like larva formation*
NGM plates NGM minus cholesterol plates
Strain20°C25°C27°C20°C25°C
Wild type(N2)0% (554)0% (701)19.4% (676)0% (439)0% (319)
unc-31(e169) NDND99.3% (450)0% (496)0% (193)
sdf-9(ut157) 0% (451)14.0% (563)25.0% (468)41.0% (227)87.6% (177)
sdf-9(ut163) 0% (434)35.1% (496)77.3% (441)2.2% (491)97.5% (278)
sdf-9(ut169) 0% (319)8.5% (520)44.8% (495)63.5% (189)96.0% (149)
sdf-9(ut174) 0% (499)0.8% (488)4.5% (492)12.4% (225)79.9% (209)
sdf-9(ut187) 0% (549)33.9% (360)36.6% (481)51.7% (323)99.3% (280)
Dauer-like larva formation* at 27°C
Cholesterol concentration
Strain5 μg/ml (normal NGM)10 μg/ml25 μg/ml50 μg/ml
Wild type(N2)1.1% (278)0.9% (428)0.2% (527)0% (430)
sdf-9(ut163) 75.4% (337)49.1% (334)23.1% (412)49.4% (385)
sdf-9(ut187) 45.7% (243)4.4% (387)2.1% (423)3.0% (335)
  • The reason for the considerably different results between ut169 and ut174, whose mutations turned out to be identical by DNA sequencing, is unknown.

  • * The numbers in parentheses show the numbers of animals scored

  • The data at 27°C include both dauer-like larvae and normal dauer larvae

  • Not determined