Table 4.

UNC-71 functions cell non-autonomously in D neuron axon guidance

A
Rescuing activity of Max phenotypes*
Tissue and cell typesTransgene promoter+++ (0-10%)++ (10-20%)+ (20-40%)No rescue (>40%)
Hypodermal cells, neurons and excretory canal cellsPunc-1153020
Hypodermal cellsPe15*20041
NeuronsPunc-1190032
NeuronsPunc-330231
NeuronsPF25B3.30122
InterneuronsPglr-10026
D neuronsPunc-250009
D neuronsPunc-300003
Body wall musclesPmyo-30005
B
Rescuing activity of Max phenotypes*
Constructs+++ (0-10%)++ (10-20%)+ (20-40%)No rescue (>40%)
Punc-115-UNC-713020
Punc-115-UNC-71::GFP1300
Pe15*2-UNC-710041
Pe15*2-UNC-71::GFP0130
Punc-115-UNC-71(M-)0006
Punc-115-UNC-71(ECD)0004
Punc-71-UNC-7152210
  • (A) Rescue of unc-71(ju156) D neuron axonal defects by tissue specific promoters driving the unc-71 minigene. unc-71(ju156) animals display 48±8% axon guidance defects, scored as `no rescue'.

    (B) Functional analysis of UNC-71. UNC-71(M-), metalloprotease domain deletion construct; UNC-71(ECD), extracellular domains of UNC-71 only. Twenty to 50 animals were scored for each transgenic line, and the average percentage was presented.

  • * Degree of rescue is represented by % axon dorsal guidance defects. The dorsal guidance defects of the D neuron axons, visualized by juIs76, were quantified by counting the number of commissures from DD2-6 and VD1,3-13 that did not reach the dorsal cord within a single animal, which was then divided by 17 to convert to the percentage of defects

  • DNAs were injected at 20 ng/μl and 100 ng/μl, and the results were similar. Only the results from the 20 ng/μl DNA injection are presented