Table 1.

Tissue-specific rescue of egl-15

Extent of rescue of egl-15(Soc) lines
Transgene promoter‡‡++++/-- -nRescued egl-15(Let) lines% Clr phenotype in egl-15(neu*) F1s§ (n)
(A) Interchangeability of the egl-15 and clr-1 promoters Pegl-15 300035/584.2% (329)
Pclr-1 032052/225.3% (645)
(B) Expression of egl-15 driven by the e15 element PΔpes-10 00066NDND
Pe15*1 01135NDND
Pe15*2 700074/484.3% (637)
(C) Tissue-specific expression of egl-15Pdpy-7 (hypodermis)4**00042/299.6% (479)
Prol-6 (hypodermis)4413122/294.4% (621)
Paex-3 (neurons)00015152/2††0.4% (275)
Punc-14 (neurons)100011/166.8% (277)
Punc-54 (body wall muscles)00012120/20% (369)
  • ‡‡ Major site of expression of the tissue-specific promoter is indicated in parentheses.

  • egl-15(Soc) rescue assays were performed in a clr-1(e1745ts); egl-15(n1783) background. At the non-permissive temperature (25°C), egl-15-rescued animals are Clr, whereas non-rescued animals retain the Soc phenotype. Rescuing activity is classified as strong (++), intermediate (+), weak (+/-), or no rescue (-). n, the number of transgenic lines scored.

  • Transgenic lines were isolated in an unc-74/szT1; egl-15(n1456)/szT1 background; lines were scored as rescued if the parental line could segregate a viable unc-74; egl-15(n1456) strain bearing the extrachromosomal array.

  • § Promoter::egl-15(neu*) constructs were injected into wild-type animals. F1 transformants were identified by expression of the Pmyo-2::GFP co-transformation marker, and the percentage of transformants with the Clr phenotype was determined. n, the number of F1 transformants.

  • ND, not determined.

  • ** clr-1(e1745ts); egl-15(n1783); ayEx[Pdpy-7::egl-15] animals were Clr even at the permissive temperature (15°C).

  • †† Rescued animals from both lines were Scrawny.