Table 3.

Genotypic influences on daf-9::gfp (dhIs64) hypodermal daf-9 expression

20°C 25°C
GenotypeL3L3dL3L3d
N2++++
daf-9(dh6) ++++
daf-12(rh61rh411) --
sa156 --
rh61 -+
rh273 +++-+++-
daf-2(e1368) +++-
e1370 +++--
daf-16(mgDf50) ++++
daf-7(e1372) +++--
daf-3(mgDf90) ++++
daf-5(e1386) ++++
daf-11(m47) +++-+++-
osm-6(p811) ++++-
mgDf50 mgDf90 ++++
rh61rh411 e1368 --
rh61rh411 e1370 --
rh61rh411 e1372 --
e1370 mgDf50 +++++++
e1372 e1386 +++++
  • -, no expression. +, ++, and +++ relative intensity of daf-9H expression seen at 20°C, 22.5°C and 25°C, respectively (see Fig. 3).

    Average of n≥25 animals. L3 reproductively growing larvae, L3d constitutive dauer larvae.