Table 1.

Rescue of daf-9(e1406) with extrachromosomal arrays (20°C 68 hours post egg lay)

% transgenic non-dauers (n)*
TransgeneLine 1Line 2
-0 (>200)-
daf-9p::daf-9 genomic::GFP100 (264)100 (416)
dpy-7p::daf-9 cDNA::GFP100 (412)100 (185)
sdf-9p::daf-9 cDNA::GFP97 (320)98 (557)
che-2p::daf-9 cDNA::GFP,§62 (145)44 (323)
  • * The genetic background was daf-9(e1406) dpy-7(sc27).

  • In addition to no transgene control, two control Ex[mec-7::GFP] transgenes did not rescue daf-9(e1406). No Dpy non-dauer progeny from daf-9(e1406) dpy-7(sc27)/szT1 Ex[mec-7p::GFP] was observed (total number of brood examined=37).

  • All other transgenic animals not scored as non-dauers were partial dauers.

  • § % transgenic non-dauers at 92 hours post egg lay are: line 1, 79%; line 2, 84%.