Table 1.

Quantitative analyses of marker gene expression

Targets
InjectedGli1Gli2Gli3Ptc1Ptc2NcamNgn1aNeuroDNtub
Stage 12
Gli1+MO-C10.1*0.5*36.5*135.2*0.6*1.5*1.0*14.0*
Gli1+MO-20.70.80.60.81.01.10.9
Gli1+MO-31.10.50.31.31.10.70.4
Gli2+MO-C6.3*0.5*12.2*29.2*1.0*1.6*0.5*19.8*
Gli2+MO-10.70.70.60.60.60.30.5
Gli2+MO-30.40.50.62.11.41.00.4
Gli3+MO-C0.9*13.2*10.3*18.2*1.4*4.8*2.7*4.6*
Gli3+MO-10.50.90.61.00.40.20.7
Gli3+MO-20.80.50.60.71.20.60.3
Stage 14
Gli1+MO-C21.0*1.0*39.5*84.5*0.4*7.5*7.9*4.7*
Gli1+MO-20.71.00.70.90.80.70.7
Gli1+MO-30.90.80.81.31.80.70.5
Gli2+MO-C20.1*2.7*10.4*23.0*1.3*15.1*1.4*28.8*
Gli2+MO-10.40.50.70.60.61.50.4
Gli2+MO-30.30.30.20.60.62.80.1
Gli3+MO-C6.3*28.4*10.6*12.5*2.0*16.3*3.7*8.4*
Gli3+MO-10.40.50.60.50.40.80.2
Gli3+MO-20.40.51.20.70.40.70.1
Stage 14
GLI1+MO-C6.9*5.2*1.3*20.9*64.5*1.2*2.6*0.5*2.6*
GLI1+MO-10.83.50.10.22.00.51.90.7
GLI1+MO-21.11.30.90.85.62.54.01.6
GLI1+MO-30.70.7>0>02.41.13.30.2
GLI2+MO-C19.7*5.5*2.0*18.3*17.4*6.3*4.3*16.5*22.8*
GLI2+MO-10.91.20.50.80.41.2>00.3
GLI2+MO-21.21.92.21.00.41.4>00.2
GLI2+MO-31.01.50.81.20.95.22.00.3
GLI3+MO-C9.5*22.2*3.7*10.6*4.9*4.1*15.1*4.6*34.8*
GLI3+MO-10.30.30.41.31.20.50.10.2
GLI3+MO-20.90.30.71.42.40.50.20.7
GLI3+MO-31.00.80.91.80.40.40.80.7
  • Numbers indicate fold change in endogenous gene expression in stage 12 or 14 animal caps with frog Gli or human GLI RNAs and MOs, determined by real-time RT-PCR analyses as calculated by the ΔCT method. Gene expression levels were normalized to EF1α. Changes of at least 40% are in green for activator function and in red for repressor function.

    MO-C=Gli`x'+MO-C/uninjected AC.

    MO-`x'=Gli`x'+MO-`y'/Gli`x'+MO-C.

  • * The action of injected Gli proteins.