Table 1.

Mutations in alr-1 affect gene expression in the AWA and ASG chemosensory neurons

% of sides showing expression in neuron number:
Neuron typeMarker*Genotype102
AWA odr-7::gfp Wild type10000
alr-1(oy42) 57358
alr-1(oy56) 71227
alr-1(oy42);Ex[alr-1 genomic]9631
alr-1(oy56);Ex[alr-1 genomic]9811
alr-1(oy42);Ex[alr-1p::alr-1 cDNA]8794
alr-1(oy42);Ex[alr-1p::Al cDNA]9343
ODR-7Wild type9460
alr-1(oy42) 66286
alr-1(oy56) 81127
alr-1(ok545) 78139
odr-10::gfp Wild type100<10
alr-1(oy42) 73225
alr-1(oy56) 82108
ASG ops-1::dsRed Wild type1000<1
alr-1(oy42) 8119<1
alr-1(oy56) 8911<1
alr-1(ok545) 87130
alr-1(oy42);Ex[alr-1 genomic]9820
alr-1(oy42);Ex[alr-1p::alr-1 cDNA]9820
alr-1(oy42);Ex[alr-1p::Al cDNA]9370
Ex[unc-30::gfp]Wild type76240
alr-1(oy42) 37630
alr-1(oy56) 61390
  • Adult animals grown at 25°C were examined. n>100 for each.

  • * ODR-7 expression was examined by staining with anti-ODR-7 antibodies (Sarafi-Reinach et al., 2001). The odr-7::gfp (Sengupta et al., 1994), odr-10::gfp (Sengupta et al., 1996) and ops-1::dsRed (Sarafi-Reinach et al., 2001) transgenes were stably integrated into the genome. unc-30::gfp (Jin et al., 1994) was present on extrachromosomal arrays, and expression from the same arrays was examined in wild-type and mutant backgrounds. Numbers shown are from strains carrying two different unc-30::gfp arrays, with the exception of alr-1(oy56) in which expression from a single array was examined

  • For extrachromosomal arrays, numbers shown are from at least two independent arrays examined in a given strain background