Table 1.

Quantitative assessment of actin stress fiber organization

GenotypeTreatmentNumber of cells analyzedNumber of stress fiber bundlesStress fiber alignmentLength
Cx43α1+/+None292.1±0.139±5°32±1 μm
Cx43α1−/−303.5±0.283±4°19±1 μm
(P<0.0001)(P<0.0001)(P<0.0001)
Cx43α1+/+Recovery from cytochalasin*152.7±0.3n.d.n.d.
Cx43α1−/−154.5±0.2n.d.n.d.
(P<0.0001)
Nontransgenic controlNone242.2±0.132±5°34±2 μm
CMV43222.4±0.141±5°31±2 μm
Nontransgenic controlRecovery from cytochalasin*152.6±0.2n.d.n.d.
CMV43182.7±0.2n.d.n.d.
  • n.d., not determined.

  • * Analysis 1 hour after cytochalasin removal.