Table 2.

Quantitative assessment of dye coupling

Primary neighborsSecondary neighborsTertiary neighbors
Fibronectin coating*Dye filled/total cellsDye filledDye filled/total cellsDye filledDye filled/total cellsDye filled
1 μg/ml (6)23/3078±8%19/5045±15%4/775.1±5%
15 μg/ml (5)27/3289±8%17/5928±8%1/651.7±2%
50 μg/ml (6)25/2887±6%11/4824±8%1/682.4±2%
  • * Number in parenthesis indicates number of impalements.

  • Sum of dye-filled cells/total cells from all impalements.

  • Mean±s.e.m. obtained from averaging the percentage of dye filled cells obtained with each impalement.