Table 2.

Effects of RNAi of GATA transcription factors on intestinal GFP markers

p::GFP reporters
GATA TFsgst-42elo-6D2030.5ZK970.2C25E10.8B0218.8
elt-2 Reduced (P<0.001)No changeNo changeReduced (P<0.001)Reduced (P<0.001)Reduced (P<0.001)
end-3 Reduced (P<0.001)No changeNo changeReduced (P<0.001)Reduced (P<0.001)No change
end-1 No changeNo changeNo changeReduced (P<0.001)Reduced (P<0.001)No change
elt-3 No changeNo changeNo changeNo changeReduced (P<0.001)No change
elt-1 * No changeNo changeNo changeReduced (P<0.001)Reduced (P<0.001)No change
egl-18 * Reduced (P<0.001)No changeReduced (P<0.001)Reduced (P<0.001)Reduced (P<0.001)Reduced (P<0.001)
elt-6 * No changeNo changeNo changeReduced (P<0.001)Reduced (P<0.001)No change
  • * elt-6, elt-1 and egl-18 GFP reporters are not expressed in the intestine, and yet RNAi treatment of these genes appears to regulate intestinal GFP reporters. Either these genes are indeed expressed in the intestine (GFP expression may not reflect true in vivo patterns) or the regulation is indirect.