Table 2.

Influence of GIS, ZFP8 and GIS2 on inflorescence trichome initiation

GenotypeSepalsSecond caul. leafThird caul. leafSecond br.Third br.Stem (first internode)
Wild type58.5 (0:9)54.3 (2.6)9.0 (0.7)42.5 (2.6)10.5 (1.7)115.8 (1.2)
RNAi control60.1 (1.0)55.1 (2.0)9.7 (0.7)43.6 (1.9)12.7 (2.1)119.1 (1.8)
gis 32.6 (0.7)45.6 (2.6)7.7 (0.6)0.5 (0.3)0.0 (0.0)79.7 (1.7)
zfp8 41.3 (0.9)23.9 (2.0)3.2 (0.4)38.7 (3.5)5.6 (1.2)101.6 (2.4)
gis2 2.2 (0.3)59.5 (1.6)7.6 (0.4)41.7 (2.1)6.1 (0.9)110.1 (1.8)
gis gis2 0.0 (0.0)41.7 (2.0)7.2 (0.4)0.1 (0.1)0.0 (0.0)80.6 (1.5)
gis ZFP8-R1 29.2 (1.5)32.0 (3.0)3.0 (0.5)0.0 (0.0)0.0 (0.0)76.7 (2.4)
gis ZFP8-R2 27.0 (1.3)27.9 (2.2)2.6 (0.4)0.0 (0.0)0.0 (0.0)75.5 (2.9)
gis2 ZFP8-R1 2.3 (0.2)28.8 (2.6)2.6 (0.3)28.0 (3.4)3.8 (0.9)98.4 (2.1)
gis2 ZFP8-R2 2.7 (0.3)31.2 (2.0)3.8 (0.5)35.5 (3.1)5.4 (1.1)101.3 (2.0)
gis ZFP8-R1 gis2 0.0 (0.0)25.7 (1.8)2.1 (0.4)0.0 (0.0)0.0 (0.0)75.2 (2.1)
gis ZFP8-R2 gis2 0.0 (0.0)28.3 (2.2)3.4 (0.4)0.0 (0.0)0.0 (0.0)74.0 (2.7)
  • Trichome production on inflorescence organs of mutants and RNAi lines that are deficient in GIS, ZFP8 and/or GIS2 function. All trichomes were counted on the first internode of branches or main stems. In the case of main stems, the values were extrapolated from average internode lengths using measurements made on a 1.5 cm-long segment 2 cm from the base Leaf trichome values were extrapolated using average leaf areas from counts obtained within a 0.6 cm2 area. Values represent averages and standard error (in parentheses) for 20 plants. RNAi control, transgenic plants that were transformed with the insert-less RNAi vector; ZFP8-R, line in which the ZFP8 gene was silenced by RNAi; caul. leaf, cauline leaf; br., branch.