Table 2.

Meiotic defects in CTCF-depleted oocytes

16 hours*
LineGenotype0 hours* % SN-type (n)1 hour* % GVBD (n)2 hours* % GVBD (n)% Ana/telo 1 (n)% PBE (n)
1Ntg68.7 (131)26.0 (50)76.0 (50)9.5 (63)55.6 (63)
Tg48.0 (98)2.0 (50)74.0 (50)16.1 (62)32.3 (62)
P<0.01P<0.001P=1P=0.3P<0.05
12Ntg64.5 (110)NDND3.2 (62)38.7 (62)
Tg57.6 (92)NDND6.2 (81)24.7 (81)
P=0.384P=0.7P=0.099
21Ntg81.5 (119)NDND4.8 (62)58.1 (62)
Tg64.9 (111)NDND12.1 (66)28.8 (66)
P<0.01P=0.21P<0.01
  • SN-type, surrounded nucleolus-type GV oocytes.

    GVBD, germinal vesicle breakdown.

    Ana/telo 1, anaphase or telophase of meiosis 1.

    PBE, polar body extrusion.

    Ntg, non-transgenic; Tg, transgenic.

    n, number of oocytes.

    ND, not determined.

  • * Germinal vesicle (GV) oocytes were cultured for the indicated number of hours.