Table 1.

Time-course analysis of actomyosin-dependent cytoplasmic streaming

DIC particle speed (μm/minute±s.d.)
Genotype*16 hours (n)20 hours (n)24 hours (n)
Wild type6.70±2.40 (10)5.40±2.20 (14)5.50±1.80 (11)
fog-2(oz40) 1.20±1.10 (11)0.44±0.86 (16)0±0 (8)
fog-2(oz40), mated 15 minutesNDND4.70±2.30 (7)
fog-2(oz40), mated 1 hourNDND5.13±0.47 (6)
fog-3(q443); acy-4(ok1806); tnEx37[acy-4(+)] NDND12.90±3.0 (9)
fog-3(q443); acy-4(ok1806) NDND0±0 (5)
fog-2(oz40), buffer injectedNDND0±0 (5)
fog-2(oz40), MSP injected, 1 hourNDND5.69±3.10 (7)
inx-22(tm1661); fog-2(oz40) ND4.88±1.6 (7)4.50±0.45 (5)§
gsa-1(RNAi) 4.08±2.0 (9)1.60±2.40 (11)0±0 (8)
acy-4(ok1806) 3.20±2.08 (10)0.55±0.90 (11)0±0 (10)
oma-1(zu405te33); oma-2(te51) 1.40±1.40 (8)1.35±1.20 (9)1.50±1.60 (9)
  • * Unmated fog-2 or fog-3 females were analyzed to eliminate sperm from the gonad, unless mated with CB4855 males.

  • Flow rates were also measured 15 minutes after MSP injection into fog-2(oz40) females. At the 15 minute timepoint, two out of seven animals exhibited flow rates of 2.35±1.20. The other five animals did not exhibit flows.

  • P<0.0001 compared to the wild type.

  • § Flow rates were taken at 30 hours post-L4. ND, not determined.