- Efficient TALEN-mediated gene targeting of chicken primordial germ cells
Summary: TALE nucleases are used to target the DDX4 (vasa) locus in chicken primordial germ cells and generate DDX4 knockouts, which provide insights into DDX4 function in early chick development.
- BMP regulates regional gene expression in the dorsal otocyst through canonical and non-canonical intracellular pathways
Summary: BMP signaling regulates the expression of Dlx5 and Hmx3, transcription factors essential for vestibular development, through the canonical pSMAD pathway and a non-canonical pathway, respectively.
- Calsyntenin 1-mediated trafficking of axon guidance receptors regulates the switch in axonal responsiveness at a choice point
Summary: Calsyntenin 1 and RabGDI contribute to cargo-specific vesicle trafficking in axonal pathfinding, leading to tightly regulated surface expression of guidance receptors at choice points.
- Differences in the spatiotemporal expression and epistatic gene regulation of the mesodiencephalic dopaminergic precursor marker PITX3 during chicken and mouse development
Summary: Analyses of the mDA neuron marker genes Pitx3 and Aldh1a1 in chick and mouse suggest important differences in how mDA neurons are generated in these species.
- Differentiation and molecular heterogeneity of inhibitory and excitatory neurons associated with midbrain dopaminergic nuclei
Summary: Tal1 and Gata transcription factors select between inhibitory GABAergic and excitatory glutamatergic fates of neurons regulating the dopaminergic system.
- The evolution of basal progenitors in the developing non-mammalian brain
Highlighted article: In the developing chick pallium, a basal progenitor population resembles mammalian cortical basal radial glia, suggesting a more ancient evolutionary origin for this cell type.
- Neural crest migration is driven by a few trailblazer cells with a unique molecular signature narrowly confined to the invasive front
Summary: Single cell mRNA profiling defines a group of leading cells in migratory neural crest. Key factors expressed in these cells regulate the behaviour of the migrating neural crest population.
- Animal models for studying neural crest development: is the mouse different?
Summary: This Spotlight article compares the advantages and disadvantages of the different vertebrate model systems available to investigate neural crest development.