- Retinoblastoma protein controls growth, survival and neuronal migration in human cerebral organoids
Summary: In human cerebral organoids, depletion of the tumor suppressor retinoblastoma protein disrupts proliferation, promotes entry into S-phase, and causes increased apoptosis and aberrant neuronal migration.
- Efficient CRISPR/Cas9-assisted gene targeting enables rapid and precise genetic manipulation of mammalian neural stem cells
Summary: Optimised strategies and protocols enable a range of complex genetic manipulations directly in mouse and human neural stem cell lines.
- Optimized inducible shRNA and CRISPR/Cas9 platforms for in vitro studies of human development using hPSCs
- Leapfrogging: primordial germ cell transplantation permits recovery of CRISPR/Cas9-induced mutations in essential genes
Summary: Germ cell transplantation from mutagenized F0 Xenopus tropicalis embryos into wild-type hosts mitigates founder lethality, allowing generation of mutant lines. This technique should be transferable to other species.
- Tracking and transforming neocortical progenitors by CRISPR/Cas9 gene targeting and piggyBac transposase lineage labeling
Summary: The CRISPR/Cas9 system is used to target three tumor suppressor genes (PTEN, NF1 and P53), alone and in combination with piggyBac transposase lineage marking, to analyze their function in the developing mouse brain.