- Efficient CRISPR/Cas9-assisted gene targeting enables rapid and precise genetic manipulation of mammalian neural stem cells
Summary: Optimised strategies and protocols enable a range of complex genetic manipulations directly in mouse and human neural stem cell lines.
- Neomorphic effects of the neonatal anemia (Nan-Eklf) mutation contribute to deficits throughout development
- Six3 dosage mediates the pathogenesis of holoprosencephaly
Summary: Analysis of a hypomorphic Six3 allele reveals that variability in Six3 dosage is sufficient to generate mice exhibiting a spectrum of holoprosencephaly forms in a non-strain-specific manner.
- iDamIDseq and iDEAR: an improved method and computational pipeline to profile chromatin-binding proteins
Summary: Critical improvements to the DamID protocol improve specificity and sensitivity in determining genome-wide protein-DNA interactions in transient or stable transgenic animal lines.
- Control of lens development by Lhx2-regulated neuroretinal FGFs
Summary: The LIM homeodomain transcription factor Lhx2 regulates FGF3, FGF9 and FGF15 and is essential for lens cell proliferation, survival and differentiation in mice.
- Sall4 controls differentiation of pluripotent cells independently of the Nucleosome Remodelling and Deacetylation (NuRD) complex
Highlighted article: Sall4 and Sall1 inhibit neural differentiation in ESCs by acting at enhancer sequences independently of the NuRD complex, and are dispensable for the maintenance of pluripotency.
- Direct neuronal reprogramming: learning from and for development
Summary: This Review discusses how developmental studies have set the stage for direct neuronal reprogramming approaches and how direct conversion can shed light on unsolved questions in developmental biology.
- Dendritic diversification through transcription factor-mediated suppression of alternative morphologies
Highlighted article: Interactions among Cut, Pdm1/2, Scalloped and Vestigial transcription factors control cell type-specific dendrite morphogenesis in Drosophila sensory neurons.
- The transcription factor SOX6 contributes to the developmental origins of obesity by promoting adipogenesis
Highlighted article: The developmental transcription factor SOX6 is a positive regulator of adipogenesis in vertebrates and is associated with the fetal origins of human obesity.