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JOURNAL ARTICLES
Relationship between Wnt-1 and En-2 expression domains during early development of normal and ectopic met-mesencephalon
L. Bally-Cuif, R.M. Alvarado-Mallart, D.K. Darnell, M. Wassef
Development 1992 115: 999-1009;
L. Bally-Cuif
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R.M. Alvarado-Mallart
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D.K. Darnell
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M. Wassef
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Summary

Grafting a met-mesencephalic portion of neural tube from a 9.5-day mouse embryo into the prosencephalon of a 2-day chick embryo results in the induction of chick En-2 (ChickEn) expression in cells in contact with the graft (Martinez et al., 1991). In this paper we investigate the possibility of Wnt-1 being one of the factors involved in En-2 induction. Since Wnt-1 and En-2 expression patterns have been described as diverging during development of the met-mesencephalic region, we first compared Wnt-1 and En-2 expression in this domain by in situ hybridization in mouse embryos after embryonic day 8.5. A ring of Wnt-1-expressing cells is detected encircling the neural tube in the met-mesencephalic region at least until day 12.5. This ring consistently overlapped with the En-2 expression domain, and corresponds to the position of this latter gene's maximal expression. We subsequently studied ChickEn ectopic induction in chick embryos grafted with various portions of met-mesencephalon. When the graft originated from the level of the Wnt-1-positive ring, ChickEn induction was observed in 71% of embryos, and in these cases correlated with Wnt-1 expression in the grafted tissue. In contrast, this percentage dropped significantly when the graft was taken from more rostral or caudal parts of the mesencephalic vesicle. Taken together, these results are compatible with a prolonged role of Wnt-1 in the specification and/or development of the met-mesencephalic region, and show that Wnt-1 could be directly or indirectly involved in the regulation of En-2 expression around the Wnt-1-positive ring during this time. We also provide data on the position of the Wnt-1-positive ring relative to anatomical boundaries in the neural tube, which suggest a more general role for the Wnt-1 protein as a positional signal involved in organizing the met-mesencephalic domain.

REFERENCES

    1. Bradley R. S.,
    2. Brown A. M. C.
    (1990) The proto-oncogene int-1 encodes a secreted protein associated with the extracellular matrix. EMBO J 9, 1569–1575
    OpenUrlPubMedWeb of Science
    1. Davidson D. R.,
    2. Crawley A.,
    3. Hill R. E.,
    4. Tickle C.
    (1991) Position-dependent expression of two related homeobox genes in developing vertebrate limbs. Nature 352, 429–431
    OpenUrlCrossRefPubMedWeb of Science
    1. Davis C. A.,
    2. Joyner A. L.
    (1988) Expression patterns of the homeobox-containing genes En-1 and En-2 and the proto-oncogene int-1 diverge during mouse development. Genes Dev 2, 1736–1744
    OpenUrlAbstract/FREE Full Text
    1. Davis C. A.,
    2. Noble-Topham S. E.,
    3. Rossant J.,
    4. Joyner A. L.
    (1988) Expression of the homeobox-containing gene En-2 delineates a specific region in the developing mouse brain. Genes Dev 2, 361–371
    OpenUrlAbstract/FREE Full Text
    1. Di Nardo S.,
    2. Sher E.,
    3. Heemskerk-Jongens J.,
    4. Kassis J. A.,
    5. O'Farrell P. H.
    (1988) Two-tiered regulation of spatially patterned gene expression during Drosophila embryogenesis. Nature 332, 604–609
    OpenUrlCrossRefPubMed
    1. Fontaine B.,
    2. Changeux J.-P.
    (1989) Localization of nicotinic acetylcholine receptor a-subunit transcripts during myogenesis and motor endplate development in the chick. J. Cell. Biol 108, 1025–1037
    OpenUrlAbstract/FREE Full Text
    1. Fjöse A.,
    2. Eikon H. G.,
    3. Njolstad P. R.,
    4. Molven A.,
    5. Horvik I.
    (1988) A Zebrafish engrailed -like homeobox sequence expressed during embryogenesis. FEBS Lett 231, 355–360
    OpenUrlCrossRefPubMedWeb of Science
    1. Fung Y. K. T.,
    2. Shackleford G. M.,
    3. Brown A. M. C.,
    4. Sanders G. S.,
    5. Varmus H. E.
    (1985) Nucleotide sequence and expression in vitro of a cDNA derived from mRNA of int-1, a provirally activated mouse mammary oncogene. Mol. Cell. Biol 5, 3337–3344
    OpenUrlAbstract/FREE Full Text
    1. Gardner C. A.,
    2. Darnell D. K.,
    3. Poole S. J.,
    4. Ordahl C. P.,
    5. Barald K. F.
    (1988) Expression of an engrailed -like gene during development of the early embryonic chick nervous system. J. Neurosci. Res 21, 426–437
    OpenUrlCrossRefPubMedWeb of Science
    1. Gardner C. A.,
    2. Barald K. F.
    (1991) The cellular environment controls the expression of engrailed -like protein in the cranial neuroepithelium of quail-chick chimeric embryos. Development 113, 1037–1048
    OpenUrlAbstract
    1. Hallonnet M. E. R.,
    2. Teillet M.-A.,
    3. Le Douarin N. M.
    (1990) A new approach to the development of the cerebellum provided by the quail/chick marker system. Development 108, 19–31
    OpenUrlAbstract
    1. Hamburger V.,
    2. Hamilton H. L. J.
    (1951) A series of normal mouse stages in the development of the chick embryo. J. Morphol 88, 49–92
    OpenUrlCrossRefPubMedWeb of Science
    1. Heemskerk J.,
    2. Di Nardo S.,
    3. Kostriken R.,
    4. O'Farrell P.
    (1991) Multiple modes of engrailed regulation in the progression towards cell fate determination. Nature 352, 404–410
    OpenUrlCrossRefPubMedWeb of Science
    1. Hemmati-Brivanlou A.,
    2. de la Torre J. R.,
    3. Holt C.,
    4. Harland R.
    1009Wnt-1and En-2in met-mesencephalonM (1991) Cephalic expression and molecular characterization of XenopusEn-2. Development 111, 715–724
    OpenUrlAbstract
    1. Jue S. F.,
    2. Bradley R. S.,
    3. Rudnicki J. A.,
    4. Varmus H. E.,
    5. Brown A. M. C.
    (1992) The mouse Wnt-1 gene can act via a paracrine mechanism in transformation of mammary epithelial cells. Mol. Cell. Biol 12, 321–328
    OpenUrlAbstract/FREE Full Text
    1. Martinez S.,
    2. Alvarado-Mallart R.-M.
    (1989) Rostral cerebellum originates from the so-called “mesencephalic” vesicle: a study using Chick/Quail chimeras. Eur. J. Neurosci 1, 549–560
    OpenUrlCrossRefPubMedWeb of Science
    1. Martinez S.,
    2. Alvarado-Mallart R.-M.
    (1990) Expression of the homeobox ChickEn gene in Chick/Quail chimeras with inverted mes-metencephalic grafts. Dev. Biol 139, 432–436
    OpenUrlCrossRefPubMedWeb of Science
    1. Martinez S.,
    2. Wassef M.,
    3. Alvarado-Mallart R.-M.
    (1991) Induction of a mesencephalic phenotype in the two-day old Chick prosencephalon is preceded by the early expression of the homeobox gene En. Neuron 6, 1–20
    OpenUrlCrossRefPubMedWeb of Science
    1. McMahon A. P.,
    2. Bradley A.
    (1990) The Wnt-1 proto-oncogene is required for development of a large region of the mouse brain. Cell 62, 1073–1085
    OpenUrlCrossRefPubMedWeb of Science
    1. Olson D. J.,
    2. Christian J. L.,
    3. Moon R. T.
    (1991) Effect of Wnt-1 and related proteins on gap junctional communication in Xenopus embryos. Science 252, 1173–1176
    OpenUrlFREE Full Text
    1. Papkoff J.,
    2. Schryver B.
    (1990) Secreted int-1 protein is associated with the cell surface. Mol. Cell Biol 10, 2723–2730
    OpenUrlAbstract/FREE Full Text
    1. Patel N. H.,
    2. Martin-Blanco E.,
    3. Coleman K. G.,
    4. Poole S. J.,
    5. Ellis M.C.,
    6. Kornberg T. B.,
    7. Goodman C. S.
    (1989) Expression of engrailed proteins in arthropods, annelids and chordates. Cell 58, 955–968
    OpenUrlCrossRefPubMedWeb of Science
    1. Rijsewijk F.,
    2. Schuermann M.,
    3. Wagenaar E.,
    4. Parren P.,
    5. Weigel D.,
    6. Nusse R.
    (1987) The Drosophila homolog of the mouse mammary oncogene int-1 is identical to the segmentation gene wingless. Cell 50, 649–657
    OpenUrlCrossRefPubMedWeb of Science
    1. Thomas K. R.,
    2. Capecchi M. R.
    (1990) Targeted disruption of the murine int-1 proto-oncogene resulting in severe abnormalities in midbrain and cerebellar development. Nature 346, 847–850
    OpenUrlCrossRefPubMedWeb of Science
    1. Thomas K. R.,
    2. Musci T. S.,
    3. Neumann P. E.,
    4. Capecchi M. R.
    (1991) Swaying is a mutant allele of the proto-oncogene Wnt-1. Cell 67, 969–976
    OpenUrlCrossRefPubMedWeb of Science
    1. Vaage S.
    (1969) The segmentation of the primitive neural tube in chick embryos (Gallus domesticus). A morphological, histochemical and autoradiographical investigation. Ergebn. Anat. Entwickl. Gesch 41, 3–.
    OpenUrlPubMed
    1. Vaage S.
    (1973) The histogenesis of the isthmic nuclei in chick embryos (Gallus domesticus). I. A morphological study. Z. Anat. Entwickl. Gesch 142, 283–314
    OpenUrlCrossRefPubMed
    1. Van den Heuvel M.,
    2. Nusse R.,
    3. Johnston P.,
    4. Lawrence P. A.
    (1989) Distribution of the wingless gene product in Drosophila embryos: a protein involved in cell-cell communication. Cell 59, 739–749
    OpenUrlCrossRefPubMedWeb of Science
    1. Wilkinson D. G.,
    2. Bailes J. A.,
    3. McMahon A. P.
    (1987) Expression of the proto-oncogene int-1 is restricted to specific neural cells in the developing mouse embryo. Cell 50, 79–88
    OpenUrlCrossRefPubMedWeb of Science
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JOURNAL ARTICLES
Relationship between Wnt-1 and En-2 expression domains during early development of normal and ectopic met-mesencephalon
L. Bally-Cuif, R.M. Alvarado-Mallart, D.K. Darnell, M. Wassef
Development 1992 115: 999-1009;
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JOURNAL ARTICLES
Relationship between Wnt-1 and En-2 expression domains during early development of normal and ectopic met-mesencephalon
L. Bally-Cuif, R.M. Alvarado-Mallart, D.K. Darnell, M. Wassef
Development 1992 115: 999-1009;

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