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JOURNAL ARTICLES
Expression and potential functions of G-protein alpha subunits in embryos of Xenopus laevis
A.P. Otte, L.L. McGrew, J. Olate, N.M. Nathanson, R.T. Moon
Development 1992 116: 141-146;

Summary

During early embryonic development, many inductive interactions between tissues depend on signal transduction processes. We began to test the possibility that G-proteins participate in the signal transduction pathways that mediate neural induction. The expression during Xenopus development of three G alpha subunits, G alpha 0, G alpha i-1 and G alpha s-1, was characterized. The three maternally expressed genes showed different expression patterns during early development. Whole-mount in situ hybridization revealed that all three genes were expressed almost exclusively in the gastrula ectoderm and predominantly in the neuroectoderm in the neurula embryo. In order to investigate the involvement of these proteins in neural induction, we overexpressed the G-protein alpha subunits by injecting the G alpha mRNAs into fertilized eggs. Overexpression of G alpha s-1 increased the ability of gastrula ectoderm to become induced to neural tissue approximately four-fold. Overexpression of G alpha 0 and G alpha i-1 had less pronounced effects on neural competence, and inhibition of the G alpha 0 and G alpha i-1 proteins by pertussis toxin did not change the neural competence of the exposed gastrula ectoderm. Overexpression of the G alpha 0 and G alpha i-1 genes did, however, inhibit the normal disappearance of the blastocoel during gastrulation, suggesting a role for these G-proteins in regulating this process. The data also suggest a specific role for the G alpha s subunit in mediating the initial phases of neural induction.

REFERENCES

    1. Bonfini L.,
    2. Karlovich C. A.,
    3. Dasgupta C.,
    4. Banerjee U.
    (1992) The son of sevenless gene product: a putative activator of Ras. Science 255, 603–606
    OpenUrlAbstract/FREE Full Text
    1. Federman A. D.,
    2. Conklin B. R.,
    3. Schrader K. A.,
    4. Reed R. R.,
    5. Bourne H. R.
    (1992) Hormonal stimulation of adenylyl cyclase through Gi -proteinsubunits. Nature 356, 159–161
    OpenUrlCrossRefPubMed
    1. Goldsmith P.,
    2. Backlund P. S.,
    3. Rossiter K.,
    4. Carter A.,
    5. Milligan G.,
    6. Unson C. G.,
    7. Spiegel A.
    (1988) Purification of heterotrimeric GTP-binding proteins from brain: identification of a novel form of Go. Biochemistry 27, 7085–7090
    OpenUrlCrossRefPubMed
    1. Goldsmith P.,
    2. Rossiter K.,
    3. Carter A.,
    4. Simonds W.,
    5. Unson C. G.,
    6. Vinitsky R.,
    7. Spiegel A.
    (1988) Identification of the GTP-binding protein encoded by Gi3 complementary DNA. J. biol. Chem 263, 6476–6479
    OpenUrlAbstract/FREE Full Text
    1. Harland R. M.
    (1991) In situ hybridization: an improved whole-mount method for Xenopus embryos. Meth. in Cell Biol 36, 685–695
    OpenUrlCrossRefPubMedWeb of Science
    1. Jones J.,
    2. Logan C. Y.,
    3. Schultz R. M.
    (1991) Changes in temporal and spatial patterns of Gi protein expression in postimplantation mouse embryos. Dev. Biol 145, 128–138
    OpenUrlCrossRefPubMedWeb of Science
    1. Kintner C. R.,
    2. Melton D. A.
    (1987) Expression of Xenopus N-CAM in ectoderm is an early response to neural induction. Development 99, 311–325
    OpenUrlAbstract
    1. Kline D.,
    2. Kopf G. S.,
    3. Lavinia F. M.,
    4. Jaffe L. A.
    (1991) Evidence for the involvement of a pertussis toxin-insensitive G-protein in egg activation of the frog, Xenopus laevis. Dev. Biol 143, 218–229
    OpenUrlCrossRefPubMedWeb of Science
    1. Kopf G. S.,
    2. Woolkalis M. J.
    (1991) ADP-ribosylation of G proteins with pertussis toxin. Meth. in Enzym 195, 257–266
    OpenUrlCrossRefPubMedWeb of Science
    1. Light D. B.,
    2. Ausiello D. A.,
    3. Stanton B. A.
    (1989) Guanine nucleotide-binding protein, i-3, directly activates a cation channel in rat renal inner medullary collecting duct cells. J. Clin. Invest 84, 352–356
    OpenUrlCrossRefPubMed
    1. Light D. B.,
    2. Ausiello D. A.,
    3. Stanton B. A.
    (1989) Gprotein gates a cation channel in renal inner medullary collecting duct cells. J. Gen. Physiol 94, 1–.
    OpenUrlAbstract/FREE Full Text
    1. Olate J.,
    2. Forquera H.,
    3. Purcell P.,
    4. Codina J.,
    5. Birnbaumer L.,
    6. Allende J. E.
    (1989) Molecular cloning and sequence determination of a cDNA coding for the-subunit of a Go-type protein of Xenopus laevis oocytes. FEBS Lett 244, 188–192
    OpenUrlCrossRefPubMed
    1. Olate J.,
    2. Martinez S.,
    3. Purcell P.,
    4. Jorquera H.,
    5. Codina J.,
    6. Birnbaumer L.,
    7. Allende J. E.
    (1990) Molecular cloning and sequence determination of four different cDNA species coding for-subunits of G-proteins from Xenopus laevis oocytes. FEBS Lett 268, 27–31
    OpenUrlCrossRefPubMed
    1. Otte A. P.,
    2. Koster C. H.,
    3. Snoek G. T.,
    4. Durston A. J.
    (1988) Protein kinase C mediates neural induction in Xenopus laevis. Nature 334, 618–620
    OpenUrlCrossRefPubMed
    1. Otte A. P.,
    2. Van Run P.,
    3. Heideveld M.,
    4. Van Driel R.,
    5. Durston A. J.
    (1989) Neural induction is mediated by cross-talk between the protein kinase C and cyclic AMP pathways. Cell 58, 641–648
    OpenUrlCrossRefPubMedWeb of Science
    1. Otte A. P.,
    2. Kramer I. J. M.,
    3. Mannesse M.,
    4. Lambrechts C.,
    5. Durston A. J.
    (1990) Characterization of protein kinase C in early Xenopus embryogenesis. Development 110, 461–470
    OpenUrlAbstract/FREE Full Text
    1. Otte A. P.,
    2. Kramer I. J. M.,
    3. Durston A. J.
    (1991) Protein kinase C and regulation of the local competence of Xenopus ectoderm. Science 251, 570–573
    OpenUrlAbstract/FREE Full Text
    1. Otte A. P.,
    2. Moon R. T.
    (1992) Protein kinase C isozymes have distinct roles in neural induction and competence in Xenopus. Cell 68, 1021–1029
    OpenUrlCrossRefPubMedWeb of Science
    1. Parks S.,
    2. Wieschaus E.
    (1991) The Drosophila gastrulation gene concertina encodes a G-like protein. Cell 64, 447–458
    OpenUrlCrossRefPubMedWeb of Science
    1. Pituello F.,
    2. Homburger V.,
    3. Saint-Jeannet J.-P.,
    4. Audigier Y.,
    5. Bockaert J.,
    6. Duprat A.-M.
    (1991) Expression of the guanine nucleotide-binding protein Go correlates with the state of neural competence in the amphibian embryo. Dev. Biol 145, 311–322
    OpenUrlCrossRefPubMedWeb of Science
    1. Sharpe C. R.,
    2. Fritz A.,
    3. De Robertis E. M.,
    4. Gurdon J. B.
    (1987) A homeobox-containing marker of posterior neural differentiation shows the importance of predetermination in neural induction. Cell 50, 749–758
    OpenUrlCrossRefPubMedWeb of Science
    1. Sharpe C. R.,
    2. Pluck A.,
    3. Gurdon J. B.
    (1989) XIF3, a Xenopus peripherin gene, requires an inductive signal for enhanced expression in anterior neural tissue. Development 107, 710–714
    OpenUrl
    1. Simon M. A.,
    2. Bowtell D. D. L.,
    3. Dodson G. S.,
    4. Laverty T. R.,
    5. Rubin G. M.
    (1991) Ras1 and a putative guanine nucleotide exchange factor perform crucial steps in signaling by the sevenless protein tyrosine kinase. Cell 67, 701–716
    OpenUrlCrossRefPubMedWeb of Science
    1. Tuft P.
    (1961) A morphogenetic effect of beta-mercaptoethanol. Distribution of water in the embryo. Nature 191, 1072–1073
    OpenUrlPubMed
    1. Tuft P.
    (1961) Role of water-regulating mechanisms in amphibian morphogenesis: A quantitative hypothesis. Nature 192, 1041–1051
    OpenUrl
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JOURNAL ARTICLES
Expression and potential functions of G-protein alpha subunits in embryos of Xenopus laevis
A.P. Otte, L.L. McGrew, J. Olate, N.M. Nathanson, R.T. Moon
Development 1992 116: 141-146;
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