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JOURNAL ARTICLES
A dominant mutation in the maize homeobox gene, Knotted-1, causes its ectopic expression in leaf cells with altered fates
L.G. Smith, B. Greene, B. Veit, S. Hake
Development 1992 116: 21-30;

Summary

Dominant mutations of the Knotted-1 (Kn1) homeobox gene of maize alter the differentiation and growth of cells associated with leaf veins. By analyzing Kn1 transcripts and KN1 protein, we show that the gene is not expressed at high levels during the development of wild-type leaves. Instead, Kn1 is expressed in apical meristems of vegetative and floral shoots, and is downregulated as leaves and floral organs are initiated. Kn1 is also expressed in relatively undifferentiated cells within developing vascular bundles, as well as ground tissue, in immature, unelongated axes of wild-type vegetative and floral shoots. In Kn1-N2 mutant plants, quantitative, but not qualitative differences are apparent in Kn1 transcripts and KN1 protein, consistent with previous observations that dominant Kn1 mutations map to non-coding regions of the gene. Kn1 is expressed ectopically in vascular bundles within developing mutant leaves in a pattern that correlates with the phenotypic alterations produced by the Kn1-N2 mutation. Thus, Kn1 apparently alters the fates of leaf cells in which it is ectopically expressed from an early stage of leaf development. Based on these observations, we hypothesize that Kn1 functions in its wild-type context as a regulator of cell determination.

REFERENCES

    1. Cabrera C. V.,
    2. Botas J.,
    3. Garcia-Bellido A.
    (1985) Distribution of Ultrabithorax proteins in mutants of Drosophila bithorax complex and its transregulatory genes. Nature 318, 569–571
    OpenUrlCrossRef
    1. Chadwick R.,
    2. Jones B.,
    3. Jack T.,
    4. McGinnis W.
    (1990) Ectopic expression from the Deformed gene triggers a dominant defect in Drosophila adult head development. Dev. Biol 141, 130–140
    OpenUrlCrossRefPubMed
    1. Cheng P. C.,
    2. Greyson R. I.,
    3. Walden D. B.
    (1983) Organ initiation and development of unisexual flowers in the tassel and ear of Zea mays. Am. J. Bot 70, 450–462
    OpenUrlCrossRefWeb of Science
    1. Freeling M.,
    2. Hake S.
    (1985) Developmental genetics of mutants that specify knotted leaves in maize. Genetics 111, 617–634
    OpenUrlAbstract/FREE Full Text
    1. Frischer L. E.,
    2. Hagen F. S.,
    3. Garber R. L.
    (1986) An inversion that disrupts the Antennapedia gene causes abnormal structure and localization of RNAs. Cell 47, 1017–1023
    OpenUrlCrossRefPubMedWeb of Science
    1. Fromm M. E.,
    2. Morrish F.,
    3. Armstrong C.,
    4. Williams R.,
    5. Thomas J.,
    6. Klein T. M.
    (1990) Inheritance and expression of chimeric genes in the progeny of transgenic maize plants. BioTechnology 8, 833–844
    OpenUrlCrossRefPubMed
    1. Gehring W.
    (1987) Homeobox genes in the study of development. Science 236, 1245–1252
    OpenUrlAbstract/FREE Full Text
    1. Gelinas D.,
    2. Postlethwaite S. N.,
    3. Nelson O. E.
    (1969) Characteriza-tion of development in maize through the use of mutants. II. The abnormal growth conditioned by the Knotted mutant. Am. J. Bot 56, 671–678
    OpenUrlCrossRefWeb of Science
    1. Hake S.
    (1992) Unravelling the knots in plant development. Trends Genet 8, 109–114
    OpenUrlCrossRefPubMedWeb of Science
    1. Hake S.,
    2. Freeling M.
    (1986) Analysis of genetic mosaics shows that the extra epidermal cell divisions in Knotted mutant maize plants are induced by adjacent mesophyll cells. Nature 320, 621–623
    OpenUrlCrossRefWeb of Science
    1. Hake S.,
    2. Vollbrecht E.,
    3. Freeling M.
    (1989) Cloning Knotted, the dominant morphological mutant in maize using Ds2 as a transposon tag. EMBO J 8, 15–22
    OpenUrlPubMedWeb of Science
    1. Hayashi S.,
    2. Scott M. P.
    (1990) What determines the specificity of action of Drosophila homeodomain proteins?. Cell 63, 883–894
    OpenUrlCrossRefPubMedWeb of Science
    1. Hoey T.,
    2. Levine M.
    (1988) Divergent homeobox proteins recognize similar DNA sequences in Drosophila. Nature 332, 858–861
    OpenUrlCrossRefPubMed
    1. Ingham P. W.
    (1988) The molecular genetics of embryonic pattern formation in Drosophila. Nature 335, 25–34
    OpenUrlCrossRefPubMedWeb of Science
    1. Klein T. M.,
    2. Wolf E. D.,
    3. Wu R.,
    4. Sanford J. C.
    (1987) High velocity microprojectiles for delivering nucleic acids into living cells. Nature 327, 70–73
    OpenUrlCrossRefWeb of Science
    1. Klein T. M.,
    2. Gradziel T.,
    3. Fromm M. E.,
    4. Sanford J. C.
    (1988) Factors influencing gene delivery into Zea mays cells by high velocity microprojectiles. BioTechnology 6, 559–563
    OpenUrlCrossRef
    1. Klein T. M.,
    2. Kornstein L.,
    3. Sanford J. C.,
    4. Fromm M. E.
    (1989) Genetic transformation of maize cells by particle bombardment. Plant Physiol 91, 440–444
    OpenUrlAbstract/FREE Full Text
    1. Langdale J. A.,
    2. Lane B.,
    3. Freeling M.,
    4. Nelson T.
    (1989) Cell lineage analysis of maize bundle sheath and mesophyll cells. Dev. Biol 133, 128–139
    OpenUrlCrossRefPubMedWeb of Science
    1. Laubengayer R. A.
    (1949) The vascular anatomy of the eight-rowed ear and tassel of Golden Bantam sweet corn. Am. J. Bot 36, 236–244
    OpenUrlCrossRefWeb of Science
    1. McGinnis W.,
    2. Krumlauf R.
    (1992) Homeobox genes and axial patterning. Cell 68, 283–302
    OpenUrlCrossRefPubMedWeb of Science
    1. Nagai K.,
    2. Thogersen H. C.
    (1987) Synthesis and sequence-specific proteolysis of hybrid proteins produced in E. coli. Meth. Enzymol 153, 461–481
    OpenUrlCrossRefPubMedWeb of Science
    1. Postlethwaite S. N.,
    2. Nelson O. E.
    (1964) Characterization of development in maize through the use of mutants. I. The Polytypic (Pt) and ramosa-1 (ra1) mutants. Am. J. Bot 51, 238–243
    OpenUrlCrossRefWeb of Science
    1. Rosenberg A. H.,
    2. Lade B. N.,
    3. Chui D.-s.,
    4. Lin S.-W.,
    5. Dunn J. J.,
    6. Studier F. W.
    (1987) Vectors for selective expression of cloned DNA's by T7 polymerase. Gene 56, 125–135
    OpenUrlCrossRefPubMedWeb of Science
    1. Rottman W. H.,
    2. Peter G. F.,
    3. Oeller P. W.,
    4. Keller J. A.,
    5. Shen N. F.,
    6. Nagy B. P.,
    7. Taylor L. P.,
    8. Campbell A. D.,
    9. Theologis A.
    (1991) 1-Aminocyclopropane-1-carboxylate synthase in tomato is encoded by a multigene family whose transcription is induced during fruit and floral senescence. J. Mol. Biol 222, 937–961
    OpenUrlCrossRefPubMedWeb of Science
    1. Sharman B. C.
    (1942) Developmental anatomy of the shoot of Zea mays L. Ann. Bot 6, 245–282
    OpenUrlFREE Full Text
    1. Sinha N.,
    2. Hake S.
    (1990) Mutant characters of Knotted maize leaves are determined in the innermost tissue layers. Dev. Biol 141, 203–210
    OpenUrlCrossRefPubMedWeb of Science
    1. Snow M.,
    2. Snow R.
    (1933) Experiments on phyllotaxis. II. The effect of displacing a primordium. Phil. Trans. Roy. Soc. B 222, 353–400
    OpenUrlCrossRef
    1. Studier F. W.,
    2. Moffatt B. A.
    (1986) Use of bacteriophage T7 RNA polymerase to direct selective high-level expression of cloned genes. J. Molec. Biol 189, 113–130
    OpenUrlCrossRefPubMedWeb of Science
    1. Sylvester A. W.,
    2. Cande W. Z.,
    3. Freeling M.
    (1990) Division and differentiation during normal and liguleless-1 maize leaf development. Development 110, 985–1000
    OpenUrlAbstract/FREE Full Text
    1. Veit B.,
    2. Vollbrecht E.,
    3. Mathern J.,
    4. Hake S.
    (1990) A tandem duplication causes the Kn1-O allele of Knotted, a dominant morphological mutant of maize. Genetics 125, 623–631
    OpenUrlAbstract/FREE Full Text
    1. Vollbrecht E.,
    2. Veit B.,
    3. Sinha N.,
    4. Hake S.
    (1991) The developmental gene Knotted-1 is a member of a maize homeobox gene family. Nature 350, 241–243
    OpenUrlCrossRefPubMedWeb of Science
    1. White R. A. H.,
    2. Akam M. E.
    (1985) Contrabithorax mutations cause inappropriate expression of Ultrabithorax products in Drosophila. Nature 318, 567–569
    OpenUrlCrossRef
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JOURNAL ARTICLES
A dominant mutation in the maize homeobox gene, Knotted-1, causes its ectopic expression in leaf cells with altered fates
L.G. Smith, B. Greene, B. Veit, S. Hake
Development 1992 116: 21-30;
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