Skip to main content
Advertisement

Main menu

  • Home
  • Articles
    • Accepted manuscripts
    • Issue in progress
    • Latest complete issue
    • Issue archive
    • Archive by article type
    • Special issues
    • Subject collections
    • Sign up for alerts
  • About us
    • About Development
    • About the Node
    • Editors and Board
    • Editor biographies
    • Travelling Fellowships
    • Grants and funding
    • Journal Meetings
    • Workshops
    • The Company of Biologists
    • Journal news
  • For authors
    • Submit a manuscript
    • Aims and scope
    • Presubmission enquiries
    • Article types
    • Manuscript preparation
    • Cover suggestions
    • Editorial process
    • Promoting your paper
    • Open Access
    • Biology Open transfer
  • Journal info
    • Journal policies
    • Rights and permissions
    • Media policies
    • Reviewer guide
    • Sign up for alerts
  • Contacts
    • Contacts
    • Subscriptions
    • Feedback
  • COB
    • About The Company of Biologists
    • Development
    • Journal of Cell Science
    • Journal of Experimental Biology
    • Disease Models & Mechanisms
    • Biology Open

User menu

  • Log in
  • Log out

Search

  • Advanced search
Development
  • COB
    • About The Company of Biologists
    • Development
    • Journal of Cell Science
    • Journal of Experimental Biology
    • Disease Models & Mechanisms
    • Biology Open

supporting biologistsinspiring biology

Development

  • Log in
Advanced search

RSS  Twitter  Facebook  YouTube 

  • Home
  • Articles
    • Accepted manuscripts
    • Issue in progress
    • Latest complete issue
    • Issue archive
    • Archive by article type
    • Special issues
    • Subject collections
    • Sign up for alerts
  • About us
    • About Development
    • About the Node
    • Editors and Board
    • Editor biographies
    • Travelling Fellowships
    • Grants and funding
    • Journal Meetings
    • Workshops
    • The Company of Biologists
    • Journal news
  • For authors
    • Submit a manuscript
    • Aims and scope
    • Presubmission enquiries
    • Article types
    • Manuscript preparation
    • Cover suggestions
    • Editorial process
    • Promoting your paper
    • Open Access
    • Biology Open transfer
  • Journal info
    • Journal policies
    • Rights and permissions
    • Media policies
    • Reviewer guide
    • Sign up for alerts
  • Contacts
    • Contacts
    • Subscriptions
    • Feedback
JOURNAL ARTICLES
Analysis of genetic mosaics in developing and adult Drosophila tissues
T. Xu, G.M. Rubin
Development 1993 117: 1223-1237;
T. Xu
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
G.M. Rubin
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • Article
  • Info & metrics
  • PDF
Loading

Summary

We have constructed a series of strains to facilitate the generation and analysis of clones of genetically distinct cells in developing and adult tissues of Drosophila. Each of these strains carries an FRT element, the target for the yeast FLP recombinase, near the base of a major chromosome arm, as well as a gratuitous cell-autonomous marker. Novel markers that carry epitope tags and that are localized to either the cell nucleus or cell membrane have been generated. As a demonstration of how these strains can be used to study a particular gene, we have analyzed the developmental role of the Drosophila EGF receptor homolog. Moreover, we have shown that these strains can be utilized to identify new mutations in mosaic animals in an efficient and unbiased way, thereby providing an unprecedented opportunity to perform systematic genetic screens for mutations affecting many biological processes.

REFERENCES

    1. Ashburner M.
    (1991) Drosophila genetic maps. Drosophila Inf. Service 69, 1–399
    OpenUrl
    1. Baker N. E.,
    2. Rubin G. M.
    (1989) Effect on eye development of dominant mutations in Drosophila homologue of the EGF receptor. Nature 340, 150–153
    OpenUrlCrossRefPubMed
    1. Baker N. E.,
    2. Rubin M. G.
    (1992) Ellipse mutations in the Drosophila homologue of the EGF receptor affect pattern formation, cell division, and cell death in eye imaginal discs. Dev. Biol 150, 381–396
    OpenUrlCrossRefPubMedWeb of Science
    1. Chou T.-B.,
    2. Perrimon N.
    (1992) Use of a yeast site-specific recombinase to produce female germline chimeras in Drosophila. Genetics 131, 643–653
    OpenUrlAbstract/FREE Full Text
    1. Clifford R. J.,
    2. Schupbach T.
    (1989) Coordinately and differentially mutable activities of torpedo, the Drosophila melanogaster homolog of the vertebrate EGF receptor gene. Genetics 123, 771–787
    OpenUrlAbstract/FREE Full Text
    1. Evan G. I.,
    2. Lewis G. K.,
    3. Ramsay G.,
    4. Bishop J. M.
    (1985) Isolation of monoclonal antibodies specific for human c-myc proto-oncogene product. Mol. Cell. Biol 5, 3610–3616
    OpenUrlAbstract/FREE Full Text
    1. Fortini M. E.,
    2. Rubin G. M.
    (1990) Analysis of cis -acting requirements of the Rh3 and Rh4 genes reveals a bipartite organization to rhodopsin promoters in Drosophila melanogaster. Genes Dev 4, 444–463
    OpenUrlAbstract/FREE Full Text
    1. Garcia-Bellido A.,
    2. Dapena J.
    (1974) Induction, detection and characterization of cell differentiation mutants in Drosophila. Mol. Gen. Genet 128, 117–130
    OpenUrlPubMed
    1. Garcia-Bellido A.,
    2. Merriam J. R.
    (1969) Cell lineage of the imaginal discs in Drosophila gynandromorphs. J. Exp. Zool 170, 61–75
    OpenUrlCrossRefPubMedWeb of Science
    1. Geyer P. K.,
    2. Corces V. G.
    (1987) Separate regulatory elements are responsible for the complex pattern of tissue-specific and developmental transcription of the yellow locus in Drosophila melanogaster. Genes Dev 1, 996–1004
    OpenUrlAbstract/FREE Full Text
    1. Golic K. G.
    (1991) Site-specific recombination between homologous chromosomes in Drosophila. Science 252, 958–961
    OpenUrlAbstract/FREE Full Text
    1. Golic K. G.,
    2. Lindquist S.
    (1989) The FLP recombinase of yeast catalyzes site-specific recombination in the Drosophila genome. Cell 59, 499–509
    OpenUrlCrossRefPubMedWeb of Science
    1. Hopp T. P.,
    2. Prickett K. S.,
    3. Price V. L.,
    4. Libby R. T.,
    5. March C. J.,
    6. Cerretti D. P.,
    7. Urdal D. L.,
    8. Conlon P. J.
    (1988) A short polypeptide marker sequence useful for recombinant protein identification and purification. Bio. Technol 6, 1204–1210
    OpenUrlCrossRef
    1. Hotta Y.,
    2. Benzer S.
    (1972) Mapping of behaviour in Drosophila mosaics. Nature 240, 527–535
    OpenUrlCrossRefPubMed
    1. Jan L. Y.,
    2. Jan Y. N.
    (1982) Antibodies to horseradish peroxidase as specific neuronal markers in Drosophila and grasshopper embryos. Proc. Natl. Acad. Sci. USA 79, 2700–2704
    OpenUrlAbstract/FREE Full Text
    1. Janning W.
    (1972) Aldehyde-oxidase as a cell marker for internal organs in Drosophila melanogaster. Naturwissenschaften 59, 516–517
    OpenUrlPubMed
    1. Kankel D. R.,
    2. Hall J. C.
    (1976) Fate mapping of nervous system and other internal tissues in genetic mosaics of Drosophila melanogaster. Dev. Biol 48, 1–24
    OpenUrlCrossRefPubMedWeb of Science
    1. Karess R. E.,
    2. Rubin G. M.
    (1984) Analysis of P transposable element functions in Drosophila. Cell 38, 135–146
    OpenUrlCrossRefPubMedWeb of Science
    1. Lawrence P. A.
    (1981) A general cell marker for clonal analysis of Drosophila development. J. Embryol. Exp. Morph 64, 321–332
    OpenUrlPubMed
    1. Lis J. T.,
    2. Simon J. A.,
    3. Sutton C. A.
    (1983) New heat shock puffs and beta-galactosidase activity resulting from transformation of Drosophila with an hsp70-lacZ hybrid gene. Cell 35, 403–410
    OpenUrlCrossRefPubMedWeb of Science
    1. McLeod M.,
    2. Volkert F.,
    3. Broach J.
    (1984) Components of the site-specific recombination system encoded by the yeast 2-micro circle. Cold Spring Harbor Symp. Quant. Biol 49, 779–787
    OpenUrlAbstract/FREE Full Text
    1. Morgan T. H.
    (1914) Mosaics and gynandromorphs in Drosophila. Proc. Soc. Exp. Biol. Med 11, 171–172
    OpenUrlAbstract/FREE Full Text
    1. Nusslein-Volhard C.,
    2. Wieschaus E.
    (1980) Mutations affecting segment number and polarity in Drosophila. Nature 287, 795–801
    OpenUrlCrossRefPubMedWeb of Science
    1. O'Hare K.,
    2. Rubin G. M.
    (1983) Structures of P transposable elements and their sites of insertion and excision in the Drosophila melanogaster genome. Cell 34, 25–35
    OpenUrlCrossRefPubMedWeb of Science
    1. O'Kane C. J.,
    2. Gehring W. J.
    (1987) Detection in situ of genomic regulatory elements in Drosophila. Proc. Natl. Acad. Sci. USA 84, 9123–9127
    OpenUrlAbstract/FREE Full Text
    1. Patterson J. T.
    (1929) The production of mutations in somatic cells of Drosophila melanogaster by means of X-rays. J. Exp. Zool 53, 327–372
    OpenUrlCrossRefWeb of Science
    1. Price J. V.,
    2. Clifford R. J.,
    3. Schupbach T.
    (1989) The maternal ventralizing locus torpedo is allelic to faint little ball, an embryonic lethal, and encodes the Drosophila EGF receptor homolog. Cell 56, 1085–1092
    OpenUrlCrossRefPubMed
    1. Ready D. F.
    (1989) A multifaceted approach to neural development. Trends Neurosci 12, 102–110
    OpenUrlCrossRefPubMedWeb of Science
    1. Ready D. F.,
    2. Hanson T. E.,
    3. Benzer S.
    (1976) Development of the Drosophila retina, a neurocrystalline lattice. Dev. Biol 53, 217–240
    OpenUrlCrossRefPubMedWeb of Science
    1. Rebay I.,
    2. Fleming R. J.,
    3. Fehon R. G.,
    4. Cherbas L.,
    5. Cherbas P.,
    6. Artavanis-Tsakonas S.
    (1991) Specific EGF repeats of Notch mediate interactions with Delta and Serrate: Implications for Notch as multifunctional receptor. Cell 67, 687–699
    OpenUrlCrossRefPubMedWeb of Science
    1. Rio D. C.,
    2. Laski F. A.,
    3. Rubin G. M.
    (1986) Identification and immunochemical analysis of biologically active Drosophila P element transposase. Cell 44, 21–32
    OpenUrlCrossRefPubMedWeb of Science
    1. Rubin G. M.
    (1985) P transposable elements and their use as genetic tools in Drosophila. Trends Neurosci 6, 231–233
    1. Rubin G. M.,
    2. Spradling A. C.
    (1982) Genetic transformation of Drosophila with transposable element vectors. Science 218, 348–353
    OpenUrlAbstract/FREE Full Text
    1. Schejter E. D.,
    2. Shilo B.-Z.
    (1989) The Drosophila EGF receptor homolog (DER) gene is allelic to faint little ball, a locus essential for embryonic development. Cell 56, 1093–1104
    OpenUrlCrossRefPubMedWeb of Science
    1. Shilo B.-Z.,
    2. Raz E.
    (1991) Developmental control by the Drosophila EGF receptor homolog DER. Trends Genet 7, 388–392
    OpenUrlPubMed
    1. Spradling A. C.,
    2. Rubin G. M.
    (1983) The effect of chromosomal position on the expression of the Drosophila xanthine dehydrogenase gene. Cell 34, 47–57
    OpenUrlCrossRefPubMedWeb of Science
    1. Steller H.,
    2. Pirrotta V.
    (1985) A transposable P vector that confers selectable G418 resistance to Drosophila larvae. EMBO J 4, 167–171
    OpenUrlPubMedWeb of Science
    1. Stern C.
    (1936) Somatic crossing over and segregation in Drosophila melanogaster. Genetics 21, 625–730
    OpenUrlFREE Full Text
    1. Thummel C. S.,
    2. Pirrotta V.
    (1992) New pCaSpeR P element Vectors. Drosophila Inf. Service 71, 150–.
    OpenUrl
    1. Tomlinson A.
    (1985) The cellular dynamics of pattern formation in the eye of Drosophila. J. Embryol. Exp. Morph 89, 313–331
    OpenUrlPubMedWeb of Science
    1. Wharton K. A.,
    2. Johansen K. M.,
    3. Xu T.,
    4. Artavanis-Tsakonas S.
    (1985) Nucleotide sequence from the neurogenic locus notch implies aT. Xu and G. M. Rubin1237Mosaic analysis in Drosophilagene product that shares homology with proteins containing EGF-like repeats. Cell 43, 567–581
    OpenUrlCrossRefPubMedWeb of Science
    1. Wolff T.,
    2. Ready D. F.
    (1991) The beginning of pattern formation in the Drosophila compound eye: The morphogenetic furrow and the second mitotic wave. Development 113, 841–850
    OpenUrlAbstract
    1. Xu T.,
    2. Artavanis-Tsakonas S.
    (1990) deltex, a locus interacting with the neurogenic genes, Notch, Delta and mastermind in Drosophila melanogaster. Genetics 126, 665–677
    OpenUrlAbstract/FREE Full Text
Previous ArticleNext Article
Back to top
Previous ArticleNext Article

This Issue

 Download PDF

Email

Thank you for your interest in spreading the word on Development.

NOTE: We only request your email address so that the person you are recommending the page to knows that you wanted them to see it, and that it is not junk mail. We do not capture any email address.

Enter multiple addresses on separate lines or separate them with commas.
Analysis of genetic mosaics in developing and adult Drosophila tissues
(Your Name) has sent you a message from Development
(Your Name) thought you would like to see the Development web site.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Share
JOURNAL ARTICLES
Analysis of genetic mosaics in developing and adult Drosophila tissues
T. Xu, G.M. Rubin
Development 1993 117: 1223-1237;
del.icio.us logo Digg logo Reddit logo Twitter logo CiteULike logo Facebook logo Google logo Mendeley logo
Citation Tools
JOURNAL ARTICLES
Analysis of genetic mosaics in developing and adult Drosophila tissues
T. Xu, G.M. Rubin
Development 1993 117: 1223-1237;

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
Alerts

Please log in to add an alert for this article.

Sign in to email alerts with your email address

Article navigation

  • Top
  • Article
  • Info & metrics
  • PDF

Related articles

Cited by...

More in this TOC section

  • even skipped is required to produce a trans-acting signal for larval neuroblast proliferation that can be mimicked by ecdysone
  • Groucho augments the repression of multiple Even skipped target genes in establishing parasegment boundaries
  • Axial skeletal patterning in mice lacking all paralogous group 8 Hox genes
Show more JOURNAL ARTICLES

Similar articles

Other journals from The Company of Biologists

Journal of Cell Science

Journal of Experimental Biology

Disease Models & Mechanisms

Biology Open

Advertisement

Kathryn Virginia Anderson (1952-2020)

Developmental geneticist Kathryn Anderson passed away at home on 30 November 2020. Tamara Caspary, a former postdoc and friend, remembers Kathryn and her remarkable contribution to developmental biology.


Zooming into 2021

In a new Editorial, Editor-in-Chief James Briscoe and Executive Editor Katherine Brown reflect on the triumphs and tribulations of the last 12 months, and look towards a hopefully calmer and more predictable year.


Read & Publish participation extends worldwide

Over 60 institutions in 12 countries are now participating in our Read & Publish initiative. Here, James Briscoe explains what this means for his institution, The Francis Crick Institute. Find out more and view our full list of participating institutions.


Upcoming special issues

Imaging Development, Stem Cells and Regeneration
Submission deadline: 30 March 2021
Publication: mid-2021

The Immune System in Development and Regeneration
Guest editors: Florent Ginhoux and Paul Martin
Submission deadline: 1 September 2021
Publication: Spring 2022

Both special issues welcome Review articles as well as Research articles, and will be widely promoted online and at key global conferences.


Development presents...

Our successful webinar series continues into 2021, with early-career researchers presenting their papers and a chance to virtually network with the developmental biology community afterwards. Sign up to join our next session:

10 February
Time: 13:00 (GMT)
Chaired by: preLights

Articles

  • Accepted manuscripts
  • Issue in progress
  • Latest complete issue
  • Issue archive
  • Archive by article type
  • Special issues
  • Subject collections
  • Sign up for alerts

About us

  • About Development
  • About the Node
  • Editors and board
  • Editor biographies
  • Travelling Fellowships
  • Grants and funding
  • Journal Meetings
  • Workshops
  • The Company of Biologists

For authors

  • Submit a manuscript
  • Aims and scope
  • Presubmission enquiries
  • Article types
  • Manuscript preparation
  • Cover suggestions
  • Editorial process
  • Promoting your paper
  • Open Access
  • Biology Open transfer

Journal info

  • Journal policies
  • Rights and permissions
  • Media policies
  • Reviewer guide
  • Sign up for alerts

Contact

  • Contact Development
  • Subscriptions
  • Advertising
  • Feedback

 Twitter   YouTube   LinkedIn

© 2021   The Company of Biologists Ltd   Registered Charity 277992