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JOURNAL ARTICLES
Ectopic expression and function of the Antp and Scr homeotic genes: the N terminus of the homeodomain is critical to functional specificity
W. Zeng, D.J. Andrew, L.D. Mathies, M.A. Horner, M.P. Scott
Development 1993 118: 339-352;

Summary

The transcription factors encoded by homeotic genes determine cell fates during development. Each homeotic protein causes cells to follow a distinct pathway, presumably by differentially regulating downstream ‘target’ genes. The homeodomain, the DNA-binding part of homeotic proteins, is necessary for conferring the specificity of each homeotic protein's action. The two Drosophila homeotic proteins encoded by Antennapedia and Sex combs reduced determine cell fates in the epidermis and internal tissues of the posterior head and thorax. Genes encoding chimeric Antp/Scr proteins were introduced into flies and their effects on morphology and target gene regulation observed. We find that the N terminus of the homeodomain is critical for determining the specific effects of these homeotic proteins in vivo, but other parts of the proteins have some influence as well. The N-terminal part of the homeodomain has been observed, in crystal structures and in NMR studies in solution, to contact the minor groove of the DNA. The different effects of Antennapedia and Sex combs reduced proteins in vivo may depend on differences in DNA binding, protein-protein interactions, or both.

Reference

    1. Akam M.
    (1987) The molecular basis for metameric pattern in the Drosophila embryo. Development 101, 1–22
    OpenUrlAbstract/FREE Full Text
    1. Andrew D. J.,
    2. Scott M. P.
    (1992) Downstream of the homeotic genes. New Biologist 4, 5–15
    OpenUrlPubMedWeb of Science
    1. Balling R.,
    2. Mutter G.,
    3. Gruss P.,
    4. Kessel M.
    (1989). Craniofacial abnormalities induced by ectopic expression of the homeobox gene Hox-1.1 in transgenic mice. Cell 58, 337–347
    OpenUrlCrossRefPubMedWeb of Science
    1. Bergson C.,
    2. McGinnis W.
    (1990) An autoregulatory enhancer element of the Drosophila homeotic gene Deformed. EMBO J 9, 4287–4297
    OpenUrlPubMedWeb of Science
    1. Bermingham J. R. J.,
    2. Scott M. P.
    (1988) Developmentally regulated alternative splicing of transcripts from the Drosophila homeotic gene Antennapedia can produce four different proteins. EMBO J 7, 3211–3222
    OpenUrlPubMedWeb of Science
    1. Carroll S. B.,
    2. Laymon R. A.,
    3. McCutcheon M. A.,
    4. Riley P. D.,
    5. Scott M. P.
    (1986) The localization and regulation of Antennapedia protein expression in Drosophila embryos. Cell 47, 113–122
    OpenUrlCrossRefPubMedWeb of Science
    1. Casas-Finet J. R.,
    2. Fischer K. R.,
    3. Karpel R. L.
    (1992) Structural basis for the nucleic acid binding cooperativity of bacteriophage T4 gene 32 protein: The Lys/Arg)3 (Ser/Thr)2 (LAST) motif. Proc. Nat. Acad. Sci. U.S.A 89, 1050–1054
    OpenUrlAbstract/FREE Full Text
    1. Cribbs D. L.,
    2. Pultz M. A.,
    3. Johnson D.,
    4. Mazzulla M.,
    5. Kaufman T. C.
    (1992) Structural complexity and evolutionary conservation of the Drosophila homeotic gene proboscipedia. EMBO J 11, 1437–1449
    OpenUrlPubMed
    1. Denell R. E.,
    2. Hummels K. R.,
    3. Wakimoto B. T.,
    4. Kaufman T. C.
    (1981) Developmental studies of lethality associated with the Antennapedia gene complex in Drosophila melanogaster. Dev. Biol 81, 43–50
    OpenUrlCrossRefPubMed
    1. Duncan I. M.
    (1987) The bithorax complex. Ann. Rev. Genet 21, 285–319
    OpenUrlCrossRefPubMedWeb of Science
    1. Fasano L.,
    2. Röder L.,
    3. Core N.,
    4. Alexandre E.,
    5. Vola C.,
    6. Jacq B.,
    7. Kerridge S.
    (1991) The gene teashirt is required for the development of Drosophila embryonic trunk segments and encodes a protein with widely spaced zinc fingers. Cell 64, 63–79
    OpenUrlCrossRefPubMedWeb of Science
    1. Frischer L. E.,
    2. Hagen F. S.,
    3. Garber R. L.
    (1986) An inversion that disrupts the Antennapedia gene causes abnormal structure and localization of RNAs. Cell 47, 1017–1023
    OpenUrlCrossRefPubMedWeb of Science
    1. Gibson G.,
    2. Gehring W. J.
    (1988) Head and thoracic transformations caused by ectopic expression of Antennapedia during Drosophila development. Development 102, 657–675
    OpenUrlAbstract/FREE Full Text
    1. Gibson G.,
    2. Schier A.,
    3. LeMotte P.,
    4. Gehring W. J.
    (1990) The specificities of Sex combs reduced and Antennapedia are defined by a distinct portion of each protein that includes the homeodomain. Cell 62, 1087–1103
    OpenUrlCrossRefPubMedWeb of Science
    1. Glicksman M. A.,
    2. Brower D. L.
    (1988) Expression of the Sex combs reduced protein in Drosophila larvae. Dev. Biol 127, 113–8
    OpenUrlCrossRefPubMed
    1. Gonzalez-Reyes A.,
    2. Morata G.
    (1990) The developmental effect of overexpressing a Ubx protein in Drosophila embryo is dependent on its interaction with other homeotic products. Cell 61, 515–522
    OpenUrlCrossRefPubMedWeb of Science
    1. Gonzalez-Reyes A.,
    2. Urquia N.,
    3. Gehring W. J.,
    4. Struhl G.,
    5. Morata G.
    (1990) Are cross-regulatory interactions between homoeotic genes functionally significant?. Nature 344, 78–80
    OpenUrlCrossRefPubMed
    1. Goutte C.,
    2. Johnson A. D.
    (1988) a1 protein alters the DNA binding specificity of a2 repressor. Cell 52, 875–882
    OpenUrlCrossRefPubMedWeb of Science
    1. Hanes S. D.,
    2. Brent R.
    (1989) DNA specificity of the bicoid activator protein is determined by homeodomain recognition helix residue 9. Cell 57, 1275–1283
    OpenUrlCrossRefPubMedWeb of Science
    1. Hazelrigg T.,
    2. Kaufman T. C.
    (1983) Revertants of dominant mutations associated with the Antennapedia gene complex of Drosophila melanogaster. Genetics 105, 581–600
    OpenUrlAbstract/FREE Full Text
    1. Heuer J. G.,
    2. Kaufman T. C.
    (1992) Homeotic genes have specific functional roles in the establishment of the Drosophila embryonic peripheral nervous system. Development 115, 35–47
    OpenUrlAbstract
    1. Immergluck K.,
    2. Lawrence P. A.,
    3. Bienz M.
    (1990) Induction across germ layers in Drosophila mediated by a genetic cascade. Cell 62, 261–268
    OpenUrlCrossRefPubMedWeb of Science
    1. Johnson W. A.,
    2. Hirsh J.
    (1990) Binding of a Drosophila POU-domain protein to a sequence element regulating gene expression in specific dopaminergic neurons. Nature 343, 467–70
    OpenUrlCrossRefPubMed
    1. Kaufman T. C.,
    2. Lewis R.,
    3. Wakimoto B.
    (1980) Cytogenetic analysis of chromosome 3 in Drosophila melanogaster: the homeotic gene complex in polytene chromosomal interval 84A, B. Genetics 94, 115–133
    OpenUrlAbstract/FREE Full Text
    1. Kaufman T. C.,
    2. Seeger M. A.,
    3. Olsen G.
    (1990) Molecular and genetic organization of the Antennapedia gene complex of Drosophila melanogaster. Adv. Genet 27, 309–362
    OpenUrlCrossRefPubMed
    1. Keleher C. A.,
    2. Goutte C.,
    3. Johnson A. D.
    (1988) The yeast cell-type specific repressor2 acts cooperatively with a non-cell-type specific protein. Cell 53, 927–936
    OpenUrlCrossRefPubMedWeb of Science
    1. Kissinger C. R.,
    2. Liu B.,
    3. Martin B. E.,
    4. Kornberg T. B.,
    5. Pabo C. O.
    (1990). Crystal structure of an engrailed homeodomain-DNA complex at 2.8 Å resolution: A framework for understanding homeodomain-DNA interactions. Cell 63, 579–590
    OpenUrlCrossRefPubMedWeb of Science
    1. Koerner T. J.,
    2. Hill J. E.,
    3. Myers A. M.,
    4. Tzagoloff A.
    (1991) High-expression vectors with multiple cloning sites for construction of trpE fusion genes: pATH vectors. Methods Enzymol 194, 477–490
    OpenUrlCrossRefPubMedWeb of Science
    1. Kornfeld K.,
    2. Saint R. B.,
    3. Beachy P. A.,
    4. Harte P. J.,
    5. Peattie D. A.,
    6. Hogness D. S.
    (1989) Structure and expression of a family of Ultrabithorax mRNAs generated by alternative splicing and polyadenylation in Drosophila. Genes Dev 3, 243–258
    OpenUrlAbstract/FREE Full Text
    1. Kristie T. M.,
    2. LeBowitz J. H.,
    3. Sharp P. A.
    (1989) The octamer-binding proteins form multi-protein-DNA complexes with the HSV alpha TIF regulatory protein. EMBOJ 8, 4229–38
    OpenUrlPubMedWeb of Science
    1. Kuroiwa A.,
    2. Kloter U.,
    3. Baumgartner P.,
    4. Gehring W. J.
    (1985) Cloning of the homeotic Sex combs reduced gene on Drosophila and in situ localization of its transcripts. EMBO J 4, 3757–3764
    OpenUrlPubMed
    1. Kuziora M. A.,
    2. McGinnis W.
    (1988) Autoregulation of a Drosophila homeotic selector gene. Cell 55, 477–485
    OpenUrlCrossRefPubMedWeb of Science
    1. Lai J.-S.,
    2. Cleary M. A.,
    3. Herr W.
    (1992) A single amino acid exchange transfers VP16-induced positive control from the Oct-1 to the Oct-2 homeo domain. Genes Dev 6, 2058–2065
    OpenUrlAbstract/FREE Full Text
    1. Laughon A.,
    2. Boulet A. M.,
    3. Bermingham J. R. J.,
    4. Laymon R. A.,
    5. Scott M. P.
    (1986) Structure of transcripts from the homeotic Antennapedia gene of Drosophila melanogaste r: two promoters control the major protein-coding region. Mol. Cell. Biol 6, 4676–4689
    OpenUrlAbstract/FREE Full Text
    1. LeBowitz J. H.,
    2. Clerc R. G.,
    3. Brenowitz M.,
    4. Sharp P. A.
    (1989) The Oct-2 protein binds cooperatively to adjacent octamer sites. Genes Dev 3, 1625–38
    OpenUrlAbstract/FREE Full Text
    1. Lewis E. B.
    (1963) Genes and developmental pathways. Am. Zool 3, 33–56
    1. Lin L.,
    2. McGinnis W.
    (1992) Mapping functional specificity in the Dfd and Ubx homeo domains. Genes Dev 6, 1071–1081
    OpenUrlAbstract/FREE Full Text
    1. Lufkin T.,
    2. Mark M.,
    3. Hart C. P.,
    4. Dolle P.,
    5. LeMeur M.,
    6. Chambon P.
    (1992) Homeotic transformation of the occipital bones of the skull by ectopic expression of a homeobox gene. Nature 359, 835–841
    OpenUrlCrossRefPubMed
    1. Mahaffey J. W.,
    2. Kaufman T. C.
    (1987) Distribution of the Sex combs reduced gene products in Drosophila melanogaster. Genetics 117, 51–60
    OpenUrlAbstract/FREE Full Text
    1. Mann R. S.,
    2. Hogness D. S.
    (1990) Functional dissection of Ultrabithorax proteins in D. melanogaster. Cell 60, 597–610
    OpenUrlCrossRefPubMedWeb of Science
    1. Martinez-Arias A.
    (1986) The Antennapedia gene is required and expressed in parasegments 4 and 5 of the Drosophila embryo. EMBO J 5, 135–141
    OpenUrlPubMed
    1. Mendel D. B.,
    2. Crabtree G. R.
    (1991) HNF-1, a member of a novel class of dimerizing homeodomain proteins. J. Biol. Chem 266, 677–680
    OpenUrlFREE Full Text
    1. Mendel D. B.,
    2. Khavari P. A.,
    3. Conley P. B.,
    4. Graves M. K.,
    5. Hansen L. P.,
    6. Admon A.,
    7. Crabtree G. R.
    (1991) Characterization of a cofactor that regulates dimerization of a mammalian homeodomain protein. Science 254, 1762–1767
    OpenUrlAbstract/FREE Full Text
    1. Noordermeer J.,
    2. Johnston P.,
    3. Rijsewijk F.,
    4. Nusse R.,
    5. Lawrence P. A.
    (1992) The consequences of ubiquitous expression of the wingless gene in the Drosophila embryo. Development 116, 711–719
    OpenUrlAbstract
    1. O'Connor M. B.,
    2. Binari R.,
    3. Perkins L. A.,
    4. Bender W.
    (1988) Alternative RNA products from the Ultrabithorax domain of the bithorax complex. EMBO J 7, 435–45
    OpenUrlPubMedWeb of Science
    1. Otting G.,
    2. Qian Y. Q.,
    3. Billeter M.,
    4. Muller M.,
    5. Affolter M.,
    6. Gehring W. J.,
    7. Wuthrich K.
    (1990) Protein-DNA contacts in the structure of a homeodomain-DNA complex determined by nuclear magnetic resonance spectroscopy in solution. EMBO J 9, 3085–3092
    OpenUrlPubMedWeb of Science
    1. Pabo C. O.,
    2. Sauer R. T.
    (1984) Protein-DNA recognition. Ann. Rev. Biochem 53, 293–321
    OpenUrlCrossRefPubMedWeb of Science
    1. Pabo C. O.,
    2. Sauer R. T.
    (1992) Transcription factors: Structural families and principles of DNA recognition. Ann. Rev. Biochem 61, 1053–1095
    OpenUrlCrossRefPubMedWeb of Science
    1. Panzer S.,
    2. Weigel D.,
    3. Beckendorf S. K.
    (1992) Organogenesis in Drosophila melanogaster: Embryonic salivary gland determination is controlled by homeotic and dorsoventral patterning genes. Development 114, 49–57
    OpenUrlAbstract
    1. Pattatucci A. M.,
    2. Kaufman T. C.
    (1991) The homeotic gene Sex combs reduced of Drosophila melanogaster is differentially regulated in the embryonic and imaginal stages of development. Genetics 129, 443–461
    OpenUrlAbstract/FREE Full Text
    1. Peifer M.,
    2. Wieschaus E.
    (1990) Mutations in the Drosophila gene extradenticle affect the way specific homeo domain proteins regulate segmental identity. Genes Dev 4, 1209–1223
    OpenUrlAbstract/FREE Full Text
    1. Percival-Smith A.,
    2. Muller M.,
    3. Affolter M.,
    4. Gehring W. J.
    (1990) The interaction with DNA of wild-type and mutant fushi tarazu homeodomains. EMBO J 9, 3967–3974
    OpenUrlPubMedWeb of Science
    1. Pomerantz J. L.,
    2. Kristie T. M.,
    3. Sharp P. A.
    (1992) Recognition of the surface of a homeo domain protein. Genes Dev 6, 2047–2057
    OpenUrlAbstract/FREE Full Text
    1. Regulski M.,
    2. Dessain S.,
    3. McGinnis N.,
    4. McGinnis W.
    (1991) High-affinity binding sites for the Deformed protein are required for the function of an autoregulatory enhancer of the Deformed gene. Genes Dev 5, 278–286
    OpenUrlAbstract/FREE Full Text
    1. Reuter R.,
    2. Panganiban G. E. F.,
    3. Hoffmann F. M.,
    4. Scott M. P.
    (1990) Homeotic genes regulate the spatial expression of putative growth factors in the visceral mesoderm of Drosophila embryos. Development 110, 1031–1040
    OpenUrlAbstract/FREE Full Text
    1. Reuter R.,
    2. Scott M. P.
    (1990) Expression and functions of the homoeotic genes Antennapedia and Sex combs reduced in the embryonic midgut of Drosophila. Development 109, 289–303
    OpenUrlAbstract
    1. Riley P. D.,
    2. Carroll S. B.,
    3. Scott M. P.
    (1987) The expression and regulation of Sex combs reduced protein in Drosophila embryos. Genes Dev 1, 716–730
    OpenUrlAbstract/FREE Full Text
    1. Röder L.,
    2. Vola C.,
    3. Kerridge S.
    (1992) The role of the teashirt gene in trunk segmental identity in Drosophila. Development 115, 1017–1033
    OpenUrlAbstract
    1. Rubin G. M.,
    2. Spradling A. C.
    (1982) Genetic transformation of Drosophila with transposable element vectors. Science 218, 348–53
    OpenUrlAbstract/FREE Full Text
    1. Sanchez-Herrero E.,
    2. Vernos I.,
    3. Marco R.,
    4. Morata G.
    (1985) Genetic organization of Drosophila bithorax complex. Nature 313, 108–13
    OpenUrlCrossRefPubMed
    1. Sanger F.,
    2. Nicklen S.,
    3. Coulson A. R.
    (1977) DNA sequencing with chain-terminating inhibitors. Proc. Natl Acad. Sci.USA 74, 5463–5467
    OpenUrlAbstract/FREE Full Text
    1. Sato T.,
    2. Hayes P. H.,
    3. Denell R. E.
    (1985) Homeosis in Drosophila: Roles and spatial patterns of expression of the Antennapedia and Sex Combs Reduced loci in embryogenesis. Dev. Biol 111, 171–192
    OpenUrlCrossRef
    1. Schneuwly S.,
    2. Gehring W. J.
    (1985) Homeotic transformation of thorax into head: Developmental analysis of a new Antennapedia allele in Drosophila melanogaster. Dev. Biol 108, 377–386
    1. Schneuwly S.,
    2. Klemenz R.,
    3. Gehring W. J.
    (1987) Redesigning the body plan of Drosophila by ectopic expression of the homoeotic gene Antennapedia. Nature 325, 816–818
    OpenUrlCrossRefPubMedWeb of Science
    1. Schneuwly S.,
    2. Kuroiwa A.,
    3. Gehring W. J.
    (1987) Molecular analysis of the dominant homeotic Antennapedia phenotype. EMBO J 6, 201–206
    OpenUrlPubMedWeb of Science
    1. Scott M. P.,
    2. Weiner A. J.,
    3. Hazelrigg T. I.,
    4. Polisky B. A.,
    5. Pirrotta V.,
    6. Scalenghe F.,
    7. Kaufman T. C.
    (1983) The molecular organization of the Antennapedia locus of Drosophila. Cell 35, 763–776
    OpenUrlCrossRefPubMedWeb of Science
    1. Smith D. L.,
    2. Johnson A. D.
    (1992) A molecular mechanism for combinatorial control in yeast: MCM1 protein sets the spacing and orientation of the homeodomains of an a2 dimer. Cell 68, 133–142
    OpenUrlCrossRefPubMedWeb of Science
    1. Spradling A. C.,
    2. Rubin G. M.
    (1982) Transposition of cloned P elements into Drosophila germ line chromosomes. Science 218, 341–7
    OpenUrlAbstract/FREE Full Text
    1. Steitz T. A.
    (1990) Structural studies of protein-nucleic acid interaction: the sources of sequence-specific binding. Quart. Rev. Biophysics 23, 205–280
    OpenUrlCrossRefPubMedWeb of Science
    1. Stern S.,
    2. Herr W.
    (1991) The herpes simplex virus trans-activator VP16 recognizes the Oct-1 homeo domain: Evidence for a homeo domain recognition subdomain. Genes Dev 5, 2555–2566
    OpenUrlAbstract/FREE Full Text
    1. Stern S.,
    2. Tanaka M.,
    3. Herr W.
    (1989) The Oct-1 homeodomain directs formation of a multiprotein-DNA complex with the HSV transactivator VP16. Nature 341, 624–630
    OpenUrlCrossRefPubMedWeb of Science
    1. Stroeher V. L.,
    2. Gaiser J. C.,
    3. Garber R. L.
    (1988) Alternative RNA splicing that is spatially regulated: generation of transcripts from the Antennapedia gene of Drosophila melanogaster with different protein-coding regions. Mol. Cell. Biol 8, 4143–4154
    OpenUrlAbstract/FREE Full Text
    1. Struhl G.
    (1982) Genes controlling segmental specification in the Drosophila thorax. Proc. Natl. Acad. Sci. USA 79, 7380–4
    OpenUrlAbstract/FREE Full Text
    1. Tokunaga C.,
    2. Stern C.
    (1965) The developmental autonomy of extra sex combs in Drosophila melanogaster. Dev. Biol 11, 50–81
    OpenUrlCrossRefPubMed
    1. Treacy M. N.,
    2. He X.,
    3. Rosenfeld M. G.
    (1991) I-POU: A POU-domain protein that inhibits neuron-specific gene activation. Nature 350, 577–584
    OpenUrlCrossRefPubMed
    1. Treacy M. N.,
    2. Neilson L. I.,
    3. Turner E. E.,
    4. He X.,
    5. Rosenfeld M. G.
    (1992) Twin of I-POU: a two amino acid difference in the I-POU homeodomain distinguishes an activator from an inhibitor of transcription. Cell 68, 491–505
    OpenUrlCrossRefPubMedWeb of Science
    1. Treisman J.,
    2. Gönczy P.,
    3. Vashishtha M.,
    4. Harris E.,
    5. Desplan C.
    (1989) A single amino acid can determine the DNA-binding specificity of homeodomain proteins. Cell 59, 553–562
    OpenUrlCrossRefPubMedWeb of Science
    1. Tremml G.,
    2. Bienz M.
    (1989) Homeotic gene expression in the visceral mesoderm of Drosophila embryos. EMBO J 8, 2677–2685
    OpenUrlPubMedWeb of Science
    1. Vachon G.,
    2. Cohen B.,
    3. Pfeifle C.,
    4. McGuffin M. E.,
    5. Botas J.,
    6. Cohen S. M.
    (1992) Homeotic genes of the bithorax complex repress limb development in the abdomen of the Drosophila embryo through the target gene Distal-less. Cell 71, 437–450
    OpenUrlCrossRefPubMedWeb of Science
    1. van der Meer J. M.
    (1977) Optical clean and permanent whole mount preparation for phase constrast microscopy of cuticulat structures in insect larvae. Dros. Inf. Serv 52, 160–.
    OpenUrl
    1. Wakimoto B. T.,
    2. Kaufman T. C.
    (1981) Analysis of larval segmentation in lethal genotypes associated with the Antennapedia gene complex in Drosophila melanogaster. Dev. Biol 81, 51–64
    OpenUrlCrossRefPubMedWeb of Science
    1. Wakimoto B. T.,
    2. Turner F. R.,
    3. Kaufman T. C.
    (1984) Defects in embryogenesis in mutants associated with the Antennapedia gene complex of Drosophila melanogaster. Dev. Biol 102, 147–172
    OpenUrlCrossRefPubMedWeb of Science
    1. Weigel D.,
    2. Jäckle H.
    (1990) The fork head domain: A novel DNA binding motif of eukaryotic transcription factors?. Cell 63, 455–456
    OpenUrlCrossRefPubMedWeb of Science
    1. Weigel D.,
    2. Jurgens G.,
    3. Kuttner F.,
    4. Seifert E.,
    5. Jäckle H.
    (1989) The homeotic gene fork head encodes a nuclear protein and is expressed in the terminal regions of the Drosophila embryo. Cell 57, 645–658
    OpenUrlCrossRefPubMedWeb of Science
    1. Wolberger C.,
    2. Vershon A. K.,
    3. Liu B.,
    4. Johnson A. D.,
    5. Pabo C. O.
    (1991) Crystal structure of a MAT2 homeodomain-operator complex suggests a general model for homeodomain-DNA interactions. Cell 67, 517–528
    OpenUrlCrossRefPubMedWeb of Science
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JOURNAL ARTICLES
Ectopic expression and function of the Antp and Scr homeotic genes: the N terminus of the homeodomain is critical to functional specificity
W. Zeng, D.J. Andrew, L.D. Mathies, M.A. Horner, M.P. Scott
Development 1993 118: 339-352;
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