Advertisement
JOURNAL ARTICLES
wingless expression mediates determination of peripheral nervous system elements in late stages of Drosophila wing disc development
R.G. Phillips, J.R. Whittle
Development 1993 118: 427-438;

Summary

We have used conditional wingless genotypes to dissect the role of this gene in late stages of wing disc development. One of these genotypes (wgIL/wg-lacZ) is simultaneously a reporter of wingless transcription and temperature-sensitive for wingless function, and has allowed us to define its pattern of transcription in the absence of wingless activity. The primordia of a subset of the bristles of the notum, which develop in or immediately adjacent to wingless-expressing cells, depend upon wingless activity. The time-course of this contribution and the effect on proneural gene expression together suggest that wingless may regulate the activity of products of the achaete-scute complex in proneural clusters. wingless activity is also required at the presumptive wing margin and is a necessary precondition for the change in proliferation pattern in this region. The involvement of wingless in transducing or mediating positional signals for spatial patterning in imaginal disc development is discussed.

Reference

    1. Baker N. E.
    (1987) Molecular cloning of wingless, a segment polarity gene in Drosophila: the spatial distribution of a transcript in embryos. EMBO J 6, 1765–1773
    OpenUrlPubMedWeb of Science
    1. Baker N. E.
    (1988) Transcription of the segment-polarity gene wingless in the imaginal discs of Drosophila, and the phenotype of a pupal-lethal wg mutation. Development 103, 289–298
    OpenUrlAbstract
    1. Bourouis M.,
    2. Moore P.,
    3. Ruel L.,
    4. Grau Y.,
    5. Heitzler P.,
    6. Simpson. P.
    (1990) An early embryonic product of the gene shaggy encodes a serine/threonine protein kinase related to the CDC28/cdc2+ subfamily. EMBO J 9, 2877–2884
    OpenUrlPubMedWeb of Science
    1. Cabrera C. V.,
    2. Alonso M. C.,
    3. Johnston P.,
    4. Phillips R. G.,
    5. Lawrence P. A.
    (1987) Phenocopies induced with antisense RNA identify the wingless gene. Cell 50, 659–663
    OpenUrlCrossRefPubMedWeb of Science
    1. Campuzano S.,
    2. Modolell J.
    (1992) Patterning of the Drosophila nervous system: the achaete - scute gene complex. Trends Gen 8, 202–208
    OpenUrlCrossRefPubMedWeb of Science
    1. Cubas P.,
    2. deCelis J.-F.,
    3. Campuzano S.,
    4. Modolell J.
    (1991) Proneural clusters of achaete - scute expression and the generation of sensory organs in the Drosophila imaginal wing disc. Genes Dev 5, 996–1008
    OpenUrlAbstract/FREE Full Text
    1. Cubas P.,
    2. Modolell J.
    (1992) The extramacrochaetae gene provides information for sensory organ patterning. EMBO J 11, 3385–3393
    OpenUrlPubMedWeb of Science
    1. DiNardo S.,
    2. Sher E.,
    3. Heemskerk-Jongens J.,
    4. Kassis J.,
    5. O'Farrell P. H.
    (1988) Two-tiered regulation of spatially patterned engrailed gene expression during Drosophila embryogenesis. Nature 332, 604–609
    OpenUrlCrossRefPubMed
    1. Dougan S.,
    2. DiNardo S.
    (1992) Drosophila wingless generates cell type diversity among engrailed expressing cells. Nature 360, 347–350
    OpenUrlCrossRefPubMed
    1. Ellis H. M.,
    2. Soann D. R.,
    3. Posakony J.
    (1990) extramacrochaetae, a negative regulator of sensory organ development in Drosophila, defines a new class of helix-loop-helix proteins. Cell 61, 27–38
    OpenUrlCrossRefPubMedWeb of Science
    1. Garrell J.,
    2. Modolell J.
    (1990) The Drosophila extramacrochaetae locus, an antagonist of proneural genes that, like these genes, encodes a Helix-Loop-Helix protein. Cell, 61, 39–48
    OpenUrlCrossRefPubMedWeb of Science
    1. Ghysen A.,
    2. Dambly-Chaudiere C.
    (1988) From DNA to form: the achaete - scute complex. Genes Dev 2, 495–501
    OpenUrlFREE Full Text
    1. Greenwald I.,
    2. Rubin G.
    (1992) Making a difference: the role of cell-cell interactions in establishing separate identities for equivalent cells. Cell 68, 271–281
    OpenUrlCrossRefPubMedWeb of Science
    1. Hama C.,
    2. Ali Z.,
    3. Kornberg T. B.
    (1990) Region-specific recombination and expression are directed by portions of the Drosophila engrailed promoter. Genes Dev 4, 1079–1093
    OpenUrlAbstract/FREE Full Text
    1. Jack J.,
    2. Dorsett D.,
    3. Delotto Y.,
    4. Liu S.
    (1991) Expression of the cut locus in the Drosophila wing margin is required for cell type specification and is regulated by a distant enhancer. Development 113, 735–747
    OpenUrlAbstract
    1. Jan Y. N.,
    2. Jan L. Y.
    (1992) Neuronal specification. Current Opinion in Genetics & Development 2, 608–613
    OpenUrlCrossRefPubMed
    1. Kassis J. A.,
    2. Noll E.,
    3. Van Sickle E. P.,
    4. Odenwald W. F.,
    5. Perrimon N.
    (1992) Altering the insertional specificity of a Drosophila transposable element. Proc. Natn. Acad. Sci. USA 89, 1919–1923
    OpenUrlAbstract/FREE Full Text
    1. Martin-Belmudo M. D.,
    2. Martinez C.,
    3. Rodriguiz A.,
    4. Jimenez F.
    (1991) Distribution and function of the lethal of scute gene product during early neurogenesis in Drosophila. Development 113, 445–454
    OpenUrlAbstract
    1. Martinez-Arias A.,
    2. Baker N. A.,
    3. Ingham P. W.
    (1988) Role of segment polarity genes in the definition and maintenance of cell states in the Drosophila embryo. Development 103, 157–170
    OpenUrlAbstract
    1. Murre C.,
    2. McCaw P. S.,
    3. Vaessin H.,
    4. Caudy M.,
    5. Jan L. Y.,
    6. Jan Y. N.,
    7. Cabrera C. V.,
    8. Buskin J. N.,
    9. Hauschka S. D.,
    10. Lassar A. B.,
    11. Weintraub H.,
    12. Baltimore D.
    (1989) Interactions between heterologous helix-loop-helix proteins generate complexes that bind specifically to a common DNA sequence. Cell 58, 537–544
    OpenUrlCrossRefPubMedWeb of Science
    1. Nordemeer J.,
    2. Johnston P.,
    3. Rijsewijk F.,
    4. Nusse R.,
    5. Lawrence P. A.
    (1992) The consequences of ubiquitous expression of the wingless gene in the Drosophila embryo. Development 116, 711–719
    OpenUrlAbstract
    1. Nusse R.,
    2. Varmus H. E.
    (1982) Many tumours induced by the mouse438mammary tumor virus contain a provirus integrated in the same region of the host genome. Cell 31, 99–109
    OpenUrlCrossRefPubMedWeb of Science
    1. Nusse R.,
    2. Varmus H. E.
    (1992) Wnt genes. Cell 69, 1073–1087
    OpenUrlCrossRefPubMedWeb of Science
    1. Perrimon N.,
    2. Smouse D.
    (1989) Multiple functions of a Drosophila homeotic gene, zeste - white3, during segmentation and neurogenesis. Dev. Biol 135, 287–305
    OpenUrlCrossRefPubMed
    1. Phillips R. G.,
    2. Roberts I. J. H.,
    3. Ingham P. W.,
    4. Whittle J. R. S.
    (1990) The Drosophila segment polarity gene patched is involved in a position-signalling mechanism in imaginal discs. Development 110, 105–114
    OpenUrlAbstract
    1. Romani S.,
    2. Campuzano S.,
    3. Macagno E. R. A.,
    4. Modolell J.
    (1989) Expression of achaete and scute genes in Drosophila imaginal discs and their function in sensory organ development. Genes Dev 3, 997–1007
    OpenUrlAbstract/FREE Full Text
    1. Siegfried E.,
    2. Chou T.-B.,
    3. Perrimon N.
    (1992) wingless signaling acts through zeste-white 3, the Drosophila homolog of glycogen synthase kinase-3, to regulate engrailed and establish cell fate. Cell 71, 1167–1179
    OpenUrlCrossRefPubMedWeb of Science
    1. Skeath J. B.,
    2. Carroll S. B.
    (1991) Regulation of achaete - scute gene expression and sensory organ pattern formation in the Drosophila wing. Genes Dev 5, 984–995
    OpenUrlAbstract/FREE Full Text
    1. Sporn M. B.,
    2. Roberts A. B.
    (1988) Peptide growth factors are multifunctional. Nature 332, 217–219
    OpenUrlCrossRefPubMed
    1. Usui K.,
    2. Kimura K.-I.
    (1992) Sensory mother cells are selected from among mitotically quiescent cluster of cells in the wing disc of Drosophila. Development 116, 601–610
    OpenUrlAbstract
    1. Van Doren M.,
    2. Ellis H. M.,
    3. Posakony J. W.
    (1991) The Drosophila extramacrochaetae protein antagonizes sequence-specific DNA binding by daughterless/achaete - scute protein complexes. Development 113, 245–255
    OpenUrlAbstract
    1. Wilcox M.,
    2. Smith R. J.
    (1977) Regenerative interactions between Drosophila imaginal discs of different types. Dev. Biol 60, 287–297
    OpenUrlCrossRefPubMedWeb of Science
Back to top

 Download PDF

Email
JOURNAL ARTICLES
wingless expression mediates determination of peripheral nervous system elements in late stages of Drosophila wing disc development
R.G. Phillips, J.R. Whittle
Development 1993 118: 427-438;
del.icio.us logo Digg logo Reddit logo Twitter logo CiteULike logo Facebook logo Google logo Mendeley logo
Citation Tools
Alerts

Article navigation

Other journals from The Company of Biologists

Advertisement

An interview with Swathi Arur

Swathi Arur joined the team at Development as an Academic Editor in 2020. Her lab uses multidisciplinary approaches to understand female germline development and fertility. We met with her over Zoom to hear more about her life, her career and her love for C. elegans.

Jim Wells and Hanna Mikkola join our team of Editors

We are pleased to welcome James (Jim) Wells and Hanna Mikkola to our team of Editors. Jim joins us a new Academic Editor, taking over from Gordan Keller, and Hanna joins our team of Associate Editors. Find out more about their research interests and areas of expertise.

New funding scheme supports sustainable events

As part of our Sustainable Conferencing Initiative, we are pleased to announce funding for organisers that seek to reduce the environmental footprint of their event. The next deadline to apply for a Scientific Meeting grant is 26 March 2021.

Read & Publish participation continues to grow

“I’d heard of Read & Publish deals and knew that many universities, including mine, had signed up to them but I had not previously understood the benefits that these deals bring to authors who work at those universities.”

Professor Sally Lowell (University of Edinburgh) shares her experience of publishing Open Access as part of our growing Read & Publish initiative. We now have over 150 institutions in 15 countries and four library consortia taking part – find out more and view our full list of participating institutions.

Upcoming special issues

Imaging Development, Stem Cells and Regeneration
Submission deadline: 30 March 2021
Publication: mid-2021

The Immune System in Development and Regeneration
Guest editors: Florent Ginhoux and Paul Martin
Submission deadline: 1 September 2021
Publication: Spring 2022

Both special issues welcome Review articles as well as Research articles, and will be widely promoted online and at key global conferences.

Development presents...

Our successful webinar series continues into 2021, with early-career researchers presenting their papers and a chance to virtually network with the developmental biology community afterwards. Here, Brandon Carpenter talks about how inherited histone methylation defines the germline versus soma decision in C. elegans. 

Sign up to join our next session:

10 March
Time: TBC
Chaired by: Thomas Lecuit