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JOURNAL ARTICLES
Sex determination in the germ line of Drosophila melanogaster: activation of the gene Sex-lethal
B. Granadino, P. Santamaria, L. Sanchez
Development 1993 118: 813-816;

Summary

The germ line exhibits sexual dimorphism as do the somatic tissues. Cells with the 2X;2A chromosome constitution will follow the oogenic pathway and X;2A cells will develop into sperm. In both somatic and germ-line tissues, the sexual pathway chosen by the cells depends on the gene Sex-lethal (Sxl), whose function is continuously needed for female development. In the soma, the sex of the cells is autonomously determined by the X:A signal while, in the germ line, the sex is determined by cell autonomous (the X:A signal) and somatic inductive signals. Three X-linked genes have been identified, scute (sc), sisterless-a (sis-a) and runt (run), that determine the initial functional state of Sxl in the soma. Using pole cell transplantation, we have tested whether these genes are also needed to activate Sxl in the germ line. We found that germ cells simultaneously heterozygous for sc, sis-a, run and a deficiency for Sxl transplanted into wild-type female hosts develop into functional oocytes. We conclude that the genes sc, sis-a and run needed to activate Sxl in the soma seem not to be required to activate this gene in the germ line; therefore, the X:A signal would be made up by different genes in somatic and germ-line tissues. The Sxlf7M1/Sxlfc females do not have developed ovaries. We have shown that germ cells of this genotype transplanted into wild-type female hosts produce functional oocytes. We conclude that the somatic component of the gonads in Sxlf7M1/Sxlfc females is affected, and consequently germ cells do not develop.(ABSTRACT TRUNCATED AT 250 WORDS)

Reference

    1. Benezra R.,
    2. Davis R. L.,
    3. Lockstone D.,
    4. Turner D. L.,
    5. Weintraub H.
    (1990) The protein Id: a negative regulator of the helix-loop-helix DNA binding proteins. Cell 61, 49–59
    OpenUrlCrossRefPubMedWeb of Science
    1. Bopp D.,
    2. Bell L. R.,
    3. Cline T. W.,
    4. Schedl P.
    (1991) Developmental distribution of female-specific Sex-lethal proteins in Drosophila melanogaster. Genes Dev 5, 403–415
    OpenUrlAbstract/FREE Full Text
    1. Caudy M.,
    2. Grell E. H.,
    3. Dambly-Chaudiere C.,
    4. Ghysen A.,
    5. Jan L. Y.,
    6. Jan Y. N.
    (1988) The maternal sex determination gene daugtherless has zygotic activity necessary for the formation of peripheral neurons in Drosophila. Genes Dev 2, 843–852
    OpenUrlAbstract/FREE Full Text
    1. Cline T. W.
    (1978) Two closely-linked mutations in Drosophila melanogaster that are lethal to opposite sexes and interact with daughterless. Genetics 90, 683–698
    OpenUrlAbstract/FREE Full Text
    1. Cline T. W.
    (1983) Functioning of the genes daughterless (da) and Sex-lethal (Sxl) in Drosophila germ cells. Genetics 104, 16–17
    OpenUrl
    1. Cline T. W.
    (1984) Autoregulatory functioning of a Drosophila gene product that establishes and maintains the sexually determined state. Genetics 107, 231–277
    OpenUrlAbstract/FREE Full Text
    1. Cline T. W.
    (1986) A female specific lethal lesion in an X-linked positive regulator of the Drosophila sex determination gene Sex-lethal. Genetics 113, 641–663
    OpenUrlAbstract/FREE Full Text
    1. Cline T. W.
    (1988) Evidence that ‘ sisterless-a ‘ and ‘ sisterless-b ‘ are two of several discrete ‘numerator elements’ of the X:A sex determination signal in Drosophila that switch Sex-lethal between two alternative stable expression states. Genetics 119, 829–862
    OpenUrlAbstract/FREE Full Text
    1. Cronmiller C.,
    2. Cline T. W.
    (1987) The Drosophila sex determination gene daugtherless has different functions in the germline versus the soma. Cell 48, 479–487
    OpenUrlCrossRefPubMed
    1. Duffy J. B.,
    2. Gergen J. P.
    (1991) The Drosophila segmentation gene runt acts as a position-specific numerator element necessary for the uniform expression of the sex-determinating gene Sex-lethal. Genes and Development 5, 2176–2187
    OpenUrlAbstract/FREE Full Text
    1. Erickson J. W.,
    2. Cline T. W.
    (1991) Molecular nature of the Drosophila sex determination signal and its link to neurogenesis. Science 251, 1071–1074
    OpenUrlAbstract/FREE Full Text
    1. Murre C.,
    2. McCaw P. S.,
    3. Baltiomre D.
    (1989) A new DNA binding and dimerization motif in inmunoglobulin enhancer binding, daugtherless, MyoD and myc proteins. Cell 56, 777–783
    OpenUrlCrossRefPubMedWeb of Science
    1. Murre C.,
    2. McCaw P. S.,
    3. Vässin H.,
    4. Caudy M.,
    5. Jan Y. N.,
    6. Cabrera C. V.,
    7. Buskin J. N.,
    8. Hauschka S. D.,
    9. Lassart A. B.,
    10. Weintraub H.,
    11. Baltimore D.
    (1989) Interactions between heterologous helix-loop-helix proteins generate complexex that bind specifically to a common DNA sequence. Cell 58, 537–544
    OpenUrlCrossRefPubMedWeb of Science
    1. Nöthiger R.,
    2. Jonglez M.,
    3. Leuthold M.,
    4. Meier-Gerschwiller P.,
    5. Weber T.
    (1989) Sex determination in the germline of Drosophila depends on genetic signals and inductive somatic factors. Development 107, 505–518
    OpenUrlAbstract
    1. Oliver B.,
    2. Perrimon N.,
    3. Mahowald A. P.
    (1988) Genetic evidence that the sans-fille locus is involved in Drosophila sex determination. Genetics 120, 159–171
    OpenUrlAbstract/FREE Full Text
    1. Parkhurst S. M.,
    2. Bopp D.,
    3. Ish-Horowicz D.
    (1990) S:A ratio, the primary sex-determination signal in Drosophila, is transduced by helix-loop-helix proteins. Cell 63, 1179–1191
    OpenUrlCrossRefPubMedWeb of Science
    1. Salz H. K.,
    2. Maine E. M.,
    3. Keyes L. N.,
    4. Samuels M. E.,
    5. Cline T. W.,
    6. Schedl P.
    (1989) The Drosophila female-specific sex-determination gene, Sex-lethal, has stage-, tissue-, and sex-specific RNAs suggesting multiple modes of regulation. Genes Dev 3, 708–719
    OpenUrlAbstract/FREE Full Text
    1. Salz H. K.
    (1992) The genetic analysis of snf: a Drosophila sex determination gene requires for activation of Sex-lethal in both the germline and the soma. Genetics 130, 547–554
    OpenUrlAbstract/FREE Full Text
    1. Sánchez L.,
    2. Nöthiger R.
    (1983) Sex determination and dosage compensation in Drosophila melanogaster: production of male clones in XX females. EMBO J 2, 485–491
    OpenUrlPubMedWeb of Science
    1. Steimann-Zwicky M.
    (1988) Sex determination in Drosophila: the X chromosomal gene liz is required for Sxl activity. EMBO J 7, 3889–3898
    OpenUrlPubMedWeb of Science
    1. Steinmann-Zwicky M.,
    2. Schmid H.,
    3. Nöthiger R.
    (1989) Cell-autonomous and inductive signals can determine the sex of the germ line of Drosophila by regulating the gene Sxl. Cell 57, 157–166
    OpenUrlCrossRefPubMedWeb of Science
    1. Sun X. H.,
    2. Baltimore D.
    (1991) A inhibitory domain of E12 transcription factor prevents DNA binding in E12 homodimers but not in E12 heterodimers. Cell 64, 459–470
    OpenUrlCrossRefPubMedWeb of Science
    1. Torres M.,
    2. Sánchez L.
    (1989) The scute (T4) gene acts as a numerator element of the X:A signal that determines the state of activity of Sex-lethal in Drosophila melanogaster. EMBO J 10, 3079–3086
    OpenUrlPubMedWeb of Science
    1. Torres M.,
    2. Sánchez L.
    (1991) The sisterless-b Function of the Drosophila gene scute is restricted to the state when the X:A ratio signal determines the activity of Sex-lethal. Development 113, 715–722
    OpenUrlAbstract
    1. Torres M.,
    2. Sánchez L.
    (1992) The segmentation gene runt is needed to activate Sex-lethal, a gene that controls sex determination and dosage compensation in Drosophila. Genetical Research 59, 189–198
    OpenUrlPubMedWeb of Science
    1. Villares R.,
    2. Cabrera C. V.
    (1987) The achaete-scute gene complex of D. melanogaster: conserved domains in a subset of genes required for neurogenesis and their homology to myc. Cell 50, 415–424
    OpenUrlCrossRefPubMedWeb of Science
    1. Younger-Shepherd S.,
    2. Vaessin H.,
    3. Bier E.,
    4. Yeh Jan L.,
    5. Nung Jan Y.
    (1992) deadpan, an essential pan-neural gene encoding an HLH protein, acts as a denominator in Drosophila sex determination. Cell 70, 911–922
    OpenUrlCrossRefPubMedWeb of Science
    1. Steinmann-Zwicky M.
    (1993) Sex determination in Drosophila: sis-b, a major numerator element of the X:A ratio in the soma, does not contribute to the X:A ratio in the germ line. Development 117, 763–767
    OpenUrlAbstract
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JOURNAL ARTICLES
Sex determination in the germ line of Drosophila melanogaster: activation of the gene Sex-lethal
B. Granadino, P. Santamaria, L. Sanchez
Development 1993 118: 813-816;
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