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JOURNAL ARTICLES
GLP-1 is localized to the mitotic region of the C. elegans germ line
S.L. Crittenden, E.R. Troemel, T.C. Evans, J. Kimble
Development 1994 120: 2901-2911;

Summary

In C. elegans, germline mitosis depends on induction by the somatic distal tip cell (DTC) and on activity of the glp-1 gene. Using antibodies to GLP-1 protein, we have examined GLP-1 on western blots and by immunocytochemistry. GLP-1 is tightly associated with membranes of mitotic germline cells, supporting its identification as an integral membrane protein. Furthermore, GLP-1 is localized within the germ line to the mitotic region, consistent with the model that GLP-1 acts as a membrane receptor for the distal tip cell signal. Unexpectedly, GLP-1 and the zone of mitosis extend further than the DTC processes. We present three models by which the DTC may influence GLP-1 activity and thereby determine the zone of mitosis. The spatial restriction of GLP-1 appears to be controlled at the translational level in hermaphrodites. We suggest that down-regulation of GLP-1 may be required to effect the transition from mitosis into meiosis.

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JOURNAL ARTICLES
GLP-1 is localized to the mitotic region of the C. elegans germ line
S.L. Crittenden, E.R. Troemel, T.C. Evans, J. Kimble
Development 1994 120: 2901-2911;
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