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no tail (ntl) is the zebrafish homologue of the mouse T (Brachyury) gene
S. Schulte-Merker, F.J. van Eeden, M.E. Halpern, C.B. Kimmel, C. Nusslein-Volhard
Development 1994 120: 1009-1015;

Summary

The mouse T (Brachyury) gene is required for normal mesoderm development and the extension of the body axis. Recently, two mutant alleles of a zebrafish gene, no tail (ntl), have been isolated (Halpern, M. E., Ho., R. K., Walker, C. and Kimmel, C. B. (1993) Cell 75, 99–111). ntl mutant embryos resemble mouse T/T mutant embryos in that they lack a differentiated notochord and the caudal region of their bodies. We report here that this phenotype is caused by mutation of the zebrafish homologue of the T gene. While ntl embryos express mutant mRNA, they show no nuclear protein product. Later, expression of mRNA in mutants, but not in wild types, is greatly reduced along the dorsal midline where the notochord normally forms. This suggests that the protein is required for maintaining transcription of its own gene.

REFERENCES

    1. Ballard W. W.
    (1973) Normal embryonic stages for salmonid fishes, based onSalmo gairdneriRichardson andSalvelinus fontinalis(Mitchill). J. exp. Zool 184, 7–25
    OpenUrlCrossRefWeb of Science
    1. Buluwela L.,
    2. Forster A.,
    3. Boehm T.,
    4. Rabbitts T. H.
    (1989) A rapid procedure for colony screening using nylon filters. Nucl. Acids Res 17, 452–.
    OpenUrlFREE Full Text
    1. Chesley P.
    (1935) Development of the short-tailed mutation in the house mouse. J. Exp. Zool 70, 429–459
    OpenUrlCrossRef
    1. Culp P.,
    2. Nusslein-Volhard C.,
    3. Hopkins N.
    (1991) High-frequency germ-line transmission of plasmid DNA sequences injected into fertilized zebrafish eggs. Proc. Natn. Acad. Sci. USA 88, 7953–7957
    OpenUrlAbstract/FREE Full Text
    1. Cunliffe V.,
    2. Smith J. C.
    (1992) Ectopic mesoderm formation in Xenopus embryos caused by widespread expression of aBrachyuryhomologue. Nature 358, 427–430
    OpenUrlCrossRefPubMed
    1. Gluecksohn-Schoenheimer S.
    (1944) The development of normal and homozygousBrachyury(T/T) mouse embryos in the extraembryonic coelom of the chick. Proc. Natn. Acad. Sci. USA 30, 134–140
    OpenUrlFREE Full Text
    1. Gruneberg H. J.
    (1958) Genetical studies on the skeleton of the mouse. XXIII. The development ofBrachyuryandAnury. J. Embryol. Exp. Morph 6, 424–443
    1. Halpern M. E.,
    2. Ho. R. K.,
    3. Walker C.,
    4. Kimmel C. B.
    (1993) Induction of muscle pioneers and floor plate is distinguished by the zebrafishno tailmutation. Cell 75, 99–111
    OpenUrlCrossRefPubMedWeb of Science
    1. Hatta K.,
    2. Kimmel C. B.,
    3. Ho R. K.,
    4. Walker C.
    (1991) Thecyclopsmutation blocks specification of the floor plate of the zebrafish central nervous system. Nature 350, 339–341
    OpenUrlCrossRefPubMed
    1. Herrmann B. G.
    (1991) Expression pattern of theBrachyurygene in whole-mountTWis/TWismutant embryos. Development 113, 913–917
    OpenUrlAbstract
    1. Herrmann B. G.,
    2. Labeit S.,
    3. Poustka A.,
    4. King T. R.,
    5. Lehrach H.
    (1990) Cloning of theTgene required in mesoderm formation in the mouse. Nature 343, 617–622
    OpenUrlCrossRefPubMed
    1. Joly J.-S.,
    2. Joly C.,
    3. Schulte-Merker S.,
    4. Boulekbache H.,
    5. Condamine H.
    (1993) The ventral and posterior expression of the zebrafish homeobox geneeve1is perturbed in dorsalized and mutant embryos. Development 119, 1261–1275
    OpenUrlAbstract
    1. Kimmel C. B.
    (1989) Genetics and early development of zebrafish. Trends Genet 5, 283–288
    OpenUrlCrossRefPubMedWeb of Science
    1. Kispert A.,
    2. Herrmann B. G.
    (1993) TheBrachyurygene encodes a novel DNA binding protein. EMBO J 12, 3211–3220
    OpenUrlPubMedWeb of Science
    1. Laemmli U. K.
    (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227, 680–685
    OpenUrlCrossRefPubMed
    1. Pflugfelder G. O.,
    2. Roth H.,
    3. Poeck B.
    (1992) A homology domain shared between Drosophilaoptomotor-blindand mouseBrachyuryis involved in DNA binding. Biochem. biophys. Res. Comm 186, 918–925
    OpenUrlCrossRefPubMedWeb of Science
    1. Placzek M.,
    2. Tessier-Lavigne M.,
    3. Yamada T.,
    4. Jessell T. M.,
    5. Dodd J.
    (1990) Mesodermal control of neural cell identity: floor plate induction by the notochord. Science 250, 985–988
    OpenUrlAbstract/FREE Full Text
    1. Rashbass P.,
    2. Cooke L. A.,
    3. Herrmann B. G.,
    4. Beddington R.
    (1991) Evidence for a cell autonomous function ofBrachyuryinT/Tembryonic stem cell chimeras. Nature 353, 348–351
    OpenUrlCrossRefPubMedWeb of Science
    1. Schubiger J. L.,
    2. Germond J. E.,
    3. ten Heggeler B.,
    4. Wahli W.
    (1985) The Vi element. A transposon-like repeated DNA sequence interspersed in the vitellogenin locus of Xenopus laevis. J. Mol. Biol 186, 491–503
    OpenUrlCrossRefPubMed
    1. Schulte-Merker S.,
    2. Ho R. K.,
    3. Herrmann B. G.,
    4. Nusslein-Volhard C.
    (1992) The protein product of the zebrafish homologue of the mouseTgeneis expressed in nuclei of the germ ring and the notochord of the early embryo. Development 116, 1021–1032
    OpenUrlAbstract/FREE Full Text
    1. Smith J. C.,
    2. Price B. M. J.,
    3. Green J. B. A.,
    4. Weigel D.,
    5. Herrmann B. G.
    (1991) Expression of a Xenopus homolog ofBrachyury(T) is an immediate-early response to mesoderm induction. Cell 67, 79–87
    OpenUrlCrossRefPubMedWeb of Science
    1. van Straaten H. M. W.,
    2. Hekking J. W. M.
    (1991) Development of floor plate, neurons and axonal outgrowth pattern in the early spinal cord of the notochord-deficient chick embryo. Anat. Embryol 184, 55–63
    OpenUrlCrossRefPubMed
    1. Walker C.,
    2. Streisinger G.
    (1983) Induction of mutations by gamma-rays in pregonial germ cells of zebrafish embryos. Genetics 103, 125–136
    OpenUrlAbstract/FREE Full Text
    1. Wilkinson D. G.,
    2. Bhatt S.,
    3. Herrmann B. G.
    (1990) Expression pattern of the mouseTgene and its role in mesoderm formation. Nature 343, 657–659
    OpenUrlCrossRefPubMed
    1. Wilson V.,
    2. Rashbass P.,
    3. Beddington R. S. P.
    (1993) Chimeric analysis ofT(Brachyury) gene function. Development 117, 1321–1331
    OpenUrlAbstract
    1. Yamada T.,
    2. Placzek M.,
    3. Tanaka H.,
    4. Dodd J.,
    5. Jessell T. M.
    (1991) Control of cell pattern in the developing nervous system: Polarizing activity of the floor plate and notochord. Cell 64, 635–647
    OpenUrlCrossRefPubMedWeb of Science
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JOURNAL ARTICLES
no tail (ntl) is the zebrafish homologue of the mouse T (Brachyury) gene
S. Schulte-Merker, F.J. van Eeden, M.E. Halpern, C.B. Kimmel, C. Nusslein-Volhard
Development 1994 120: 1009-1015;
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