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JOURNAL ARTICLES
LEUNIG regulates AGAMOUS expression in Arabidopsis flowers
Z. Liu, E.M. Meyerowitz
Development 1995 121: 975-991;

Summary

LEUNIG was identified in a genetic screen designed to isolate second-site enhancer mutations of the floral homeotic mutant apetala2-1. leunig mutations not only enhance apetala2, but by themselves cause a similar but less-pronounced homeotic transformation than apetala2 mutations. leunig flowers have sepals that are transformed toward stamens and carpels, and petals that are either staminoid or absent. In situ hybridization experiments with leunig mutants revealed altered expression pattern of the floral homeotic genes APETALA1, APETALA3, PISTILLATA, and AGAMOUS. Double mutants of leunig and agamous exhibited a phenotype similar to agamous single mutants, indicating that agamous is epistatic to leunig. Our analysis suggests that a key role of LEUNIG is to negatively regulate AGAMOUS expression in the first two whorls of the Arabidopsis flower.

Reference

    1. Akam M. E.
    (1987) The molecular basis for metameric pattern in the Drosophila embryo. Development 101, 1–22
    OpenUrlAbstract/FREE Full Text
    1. Angenent G. C.,
    2. Franken J.,
    3. Busscher M.,
    4. Colombo L.,
    5. van Tunen A. J.
    (1993) Petal and stamen formation in petunia is regulated by the homeotic gene FBP1. Plant J 4, 101–112
    OpenUrlCrossRefPubMedWeb of Science
    1. Bowman J. L.,
    2. Smyth D.R.,
    3. Meyerowitz E.M.
    (1989) Genes directing flower development in Arabidopsis. Plant Cell 1, 37–52
    OpenUrlAbstract/FREE Full Text
    1. Bowman J. L.,
    2. Smyth D. R.,
    3. Meyerowitz E. M.
    (1991) Genetic interactions among floral homeotic genes of Arabidopsis. Development 112, 1–20
    OpenUrlAbstract
    1. Bowman J. L.,
    2. Sakai H.,
    3. Jack T.,
    4. Weigel D.,
    5. Mayer U.,
    6. Meyerowitz E. M.
    (1992) SUPERMAN, a regulator of floral homeotic genes in Arabidopsis. Development 114, 599–615
    OpenUrlAbstract
    1. Bowman J. L.,
    2. Alvarez J.,
    3. Weigel D.,
    4. Meyerowitz E. M.,
    5. Smyth D. R.
    (1993) Control of flower development in Arabidopsis thaliana by APETALA1 and interacting genes. Development 119, 721–743
    OpenUrlAbstract/FREE Full Text
    1. Bradley D.,
    2. Carpenter R.,
    3. Sommer H.,
    4. Hartley N.,
    5. Coen E.
    (1993) Complementary floral homeotic phenotypes result from opposite orientation of a transposon at the plena locus of Antirrhinum. Cell 72, 85–95
    OpenUrlCrossRefPubMedWeb of Science
    1. Carpenter R.,
    2. Coen E.S.
    (1990) Floral homeotic mutations produced by transposon-mutagenesis in Antirrhinum majus. Genes Dev 4, 1483–1493
    OpenUrlAbstract/FREE Full Text
    1. Coen E. S.,
    2. Meyerowitz E. M.
    (1991) The war of whorls: genetic interactions controlling flower development. Nature 353, 31–37
    OpenUrlCrossRefPubMedWeb of Science
    1. Drews G. N.,
    2. Bowman J. L.,
    3. Meyerowitz E. M.
    (1991) Negative regulation of the Arabidopsis homeotic gene AGAMOUS by the APETALA2 product. Cell 65, 991–1002
    OpenUrlCrossRefPubMedWeb of Science
    1. Gustafson-Brown C.,
    2. Savidge B.,
    3. Yanofsky M. F.
    (1994) Regulation of the Arabidopsis floral homeotic gene APETALA1. Cell 6, 131–143
    OpenUrl
    1. Herskowitz I.
    (1987) Functional inactivation of genes by dominant negative mutations. Nature 329, 219–222
    OpenUrlCrossRefPubMed
    1. Huala E.,
    2. Sussex I.M.
    (1992) LEAFY interacts with floral homeotic genes to regulate Arabidopsis floral development. Plant Cell 4, 901–913
    OpenUrlAbstract/FREE Full Text
    1. Ingham P.
    (1988) The molecular genetics of embryonic pattern formation in Drosophila. Nature 335, 25–34
    OpenUrlCrossRefPubMed
    1. Irish V.F.,
    2. Sussex I.M.
    (1990) Function of the apetala-1 gene during Arabidopsis floral development. Plant Cell 2, 741–753
    OpenUrlAbstract/FREE Full Text
    1. Jack T.,
    2. Brockman. L. L.,
    3. Meyerowitz E. M.
    (1992) The homeotic gene APETALA3 of Arabidopsisthaliana encodes a MADS box and is expressed in petals and stamens. Cell 68, 683–697
    OpenUrlCrossRefPubMedWeb of Science
    1. Jack T.,
    2. Fox G. L.,
    3. Meyerowitz E. M.
    (1994) Arabidopsis homeotic gene APETALA3 ectopic expression: transcriptional and post-transcriptional regulation determine floral organ identity. Cell 76, 703–716
    OpenUrlCrossRefPubMedWeb of Science
    1. Jofuku D.,
    2. den Boer B.,
    3. Van Montagu M.,
    4. Okamuro J.
    (1994) Control of Arabidopsis flower and seed development by the homeotic gene APETALA2. Plant Cell 6, 1211–1225
    OpenUrlAbstract/FREE Full Text
    1. Komaki M. K.,
    2. Okada K.,
    3. Nishino E.,
    4. Shimura Y.
    (1988) Isolation and characterization of novel mutants of Arabidopsisthaliana defective in flower development. Development 104, 195–203
    OpenUrlAbstract
    1. Kunst L.,
    2. Klenz J. E.,
    3. Martinez-Zapater J.,
    4. Haughn G. W.
    (1989) AP2 gene determines the identity of perianth organs in flowers of Arabidopsis thaliana. Plant Cell 1, 1195–1208
    OpenUrlAbstract/FREE Full Text
    1. Langdale J. A.,
    2. Metzler M. C.,
    3. Nelson T.
    (1987) The argentia mutation delays normal development of photosynthetic cell-types in Zea mays. Dev. Biol 122, 243–255
    OpenUrlCrossRefPubMedWeb of Science
    1. Leon-Kloosterziel K. M.,
    2. Keijzer C. J.,
    3. Koornneef M.
    (1994) A seed shape mutant of Arabidopsis that is affected in integument development. Plant Cell 6, 385–392
    OpenUrlAbstract/FREE Full Text
    1. Lewis E. B.
    (1978) A gene complex controlling segmentation in Drosophila. Nature 276, 565–570
    OpenUrlCrossRefPubMedWeb of Science
    1. Mandel M.A.,
    2. Gustafson-Brown C.,
    3. Savidge B.,
    4. Yanofsky M.F.
    (1992) Molecular characterization of the Arabidopsis floral homeotic gene APETALA1. Nature 360, 273–277
    OpenUrlCrossRefPubMedWeb of Science
    1. Norman C.,
    2. Runswick M.,
    3. Pollock R.,
    4. Treisman R.
    (1988) Isolation and properties of cDNA clones encoding SRF, a transcription factor that binds to the c-fos serum response element. Cell 55, 989–1003
    OpenUrlCrossRefPubMedWeb of Science
    1. Passmore S.,
    2. Maine G.T.,
    3. Elble R.,
    4. Christ C.,
    5. Tye B. K.
    (1988) A Saccharomycescerevisiae protein involved in plasmid maintenance is necessary for mating of MATa cells. J. Mol. Biol 204, 593–606
    OpenUrlCrossRefPubMedWeb of Science
    1. Pnueli L.,
    2. Hareven D.,
    3. Rounsley S. D.,
    4. Yanofsky M. F.,
    5. Lifshitz E.
    (1994) Isolation of the tomato AGAMOUS gene TAG1 and analysis of its homeotic role in transgenic plants. Plant Cell 6, 163–173
    OpenUrlAbstract/FREE Full Text
    1. Reinitz J.,
    2. Levin M.
    (1990) Control of the initiation of homeotic gene expression by the gap genes giant and tailless in Drosophila. Dev. Biol 140, 57–72
    OpenUrlCrossRefPubMedWeb of Science
    1. Schultz E. A.,
    2. Pickett F. B.,
    3. Haughn G. W.
    (1992) The FLO10 gene product regulates the expression domain of homeotic genes AP3 and PI in Arabidopsis flowers. Plant Cell 3, 1221–1227
    OpenUrlAbstract/FREE Full Text
    1. Schultz E. A.,
    2. Haughn G. W.
    (1993) Genetic analysis of the floral initiation process (FLIP) in Arabidopsis. Development 119, 745–765
    OpenUrlAbstract/FREE Full Text
    1. Schwarz-Sommer Z.,
    2. Huijser P.,
    3. Nacken W.,
    4. Saedler H.,
    5. Sommer H.
    (1990) Genetic control of flower development: homeotic genes of Antirrhinum Majus. Science 250, 931–936
    OpenUrlAbstract/FREE Full Text
    1. Smyth D. R.,
    2. Bowman J. L.,
    3. Meyerowitz E. M.
    (1990) Early flower development in Arabidopsis. Plant Cell 2, 755–767
    OpenUrlAbstract/FREE Full Text
    1. Shannon S.,
    2. Meeks-Wagner D. R.
    (1993) Genetic interaction that regulate inflorescence development in Arabidopsis. Plant Cell 5, 639–655
    OpenUrlAbstract/FREE Full Text
    1. Sommer H.,
    2. Beltran J. P.,
    3. Huijser P.,
    4. Pape H.,
    5. Lonnig W. E.,
    6. Saedler H.,
    7. Schwarz-Sommer Z.
    (1992) Deficiens, a homeotic gene involved in the control of flower morphogenesis in Antirrhinum majus: the protein shows homology to transcription factors. EMBO J 9, 605–613
    OpenUrlPubMedWeb of Science
    1. Tsuchimoto S.,
    2. van der Krol A. R.,
    3. Chua N. H.
    (1993) Ectopic expression of pMADS3 in transgenic petunia blind mutant. Plant cell 5, 843–853
    OpenUrlAbstract/FREE Full Text
    1. van der Krol A. R.,
    2. Brunelle A.,
    3. Tsuchimoto S.,
    4. Chua N. H.
    (1993) Functional analysis of petunia floral homeotic MADS box gene PMADS1. Gene Dev 7, 1214–1228
    OpenUrlAbstract/FREE Full Text
    1. Weigel D.,
    2. Alvarez J.,
    3. Smyth D. R.,
    4. Yanofsky M.F.,
    5. Meyerowitz E.M.
    (1992) LEAFY controls floral meristem identity in Arabidopsis. Cell 69, 843–859
    OpenUrlCrossRefPubMedWeb of Science
    1. Weigel D.,
    2. Meyerowitz E. M.
    (1993) Activation of floral homeotic genes in Arabidopsis. Science 261, 1723–1726
    1. Weigel D.,
    2. Meyerowitz E. M.
    (1994) The ABCs of floral homeotic genes. Cell 78, 203–209
    OpenUrlCrossRefPubMedWeb of Science
    1. Yanofsky M. F.,
    2. Ma H.,
    3. Bowman J. L.,
    4. Drews G. N.,
    5. Feldmann K. A.,
    6. Meyerowitz E. M.
    (1990) The protein encoded by the Arabidopsis homeotic gene AGAMOUS resembles transcription factors. Nature 346, 35–40
    OpenUrlCrossRefPubMedWeb of Science
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JOURNAL ARTICLES
LEUNIG regulates AGAMOUS expression in Arabidopsis flowers
Z. Liu, E.M. Meyerowitz
Development 1995 121: 975-991;
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