Advertisement
JOURNAL ARTICLES
Notch1 is required for the coordinate segmentation of somites
R.A. Conlon, A.G. Reaume, J. Rossant
Development 1995 121: 1533-1545;

Summary

Members of the Notch family of transmembrane receptors mediate a number of developmental decisions in invertebrates. In order to study Notch function in a vertebrate organism, we have mutated the Notch1 gene of the mouse. Notch1 gene function is required for embryonic survival in the second half of gestation. In the first half of gestation, we have found no effect of the mutation on the normal programs of neurogenesis, myogenesis or apoptosis. We conclude that Notch1 function is not essential for these processes, at least in early postimplantation development. However, we have found that somitogenesis is delayed and disorganized in Notch1 mutant embryos. We propose that Notch1 normally coordinates the process of somitogenesis, and we provide a model of how this might occur.

Reference

    1. Cagan R. L.,
    2. Ready D. F.
    (1989) Notch is required for successive cell decisions in the developing Drosophila retina. Genes Dev 3, 1099–1112
    OpenUrlAbstract/FREE Full Text
    1. Candia A. F.,
    2. Hu H.,
    3. Crosby J.,
    4. Lalley P. A.,
    5. Noden D.,
    6. Nadeau J. H.,
    7. Wright C. V. E.
    (1992) Mox-1 and Mox-2 define a novel homeobox gene subfamily and are differentially expressed during early mesodermal patterning in mouse embryos. Development 116, 1123–1136
    OpenUrlAbstract/FREE Full Text
    1. Chesley P.
    (1935) Development of the short-tailed mutant in the house mouse. J. Exp. Zool 70, 429–435
    OpenUrlCrossRefWeb of Science
    1. Coffman C.,
    2. Harris W.,
    3. Kintner C. R.
    (1990) Xotch, the Xenopus homolog of Drosophila Notch. Science 249, 1438–1441
    OpenUrlAbstract/FREE Full Text
    1. Conlon R. A.,
    2. Rossant J.
    (1992) Exogenous retinoic acid rapidly induces anterior ectopic expression of murine Hox-2 genes in vivo. Development 116, 357–368
    OpenUrlAbstract/FREE Full Text
    1. Corbin V.,
    2. Michelson A. M.,
    3. Abmayr S. M.,
    4. Neel V.,
    5. Alcamo E.,
    6. Maniatis T.,
    7. Young M. W.
    (1991) A role for the Drosophila neurogenic genes in mesoderm differentiation. Cell 67, 311–323
    OpenUrlCrossRefPubMedWeb of Science
    1. Davis C. A.,
    2. Holmyard D. P.,
    3. Millen K. J.,
    4. Joyner A. L.
    (1991) Examining pattern formation in mouse, chicken and frog embryos with an En -specific antiserum. Development 111, 287–298
    OpenUrlAbstract
    1. Deng C.-X.,
    2. Wynshaw-Boris A.,
    3. Shen M. M.,
    4. Daugherty C.,
    5. Ornitz D. M.,
    6. Leder P.
    (1994) Murine FGFR-1 is required for early postimplantation growth and axial organization. Genes Dev 8, 3045–3057
    OpenUrlAbstract/FREE Full Text
    1. Dunn L. C.,
    2. Gluecksohn-Schoenheimer S.,
    3. Bryson V.
    (1940) A new mutation in the mouse affecting spinal column and urogenital system. J. Hered 31, 343–348
    OpenUrlFREE Full Text
    1. Echelard Y.,
    2. Epstein D. J.,
    3. St-Jacques B.,
    4. Shen L.,
    5. Mohler J.,
    6. McMahon J. A.,
    7. McMahon A. P.
    (1993) Sonic hedgehog, a member of a family of putative signaling molecules, is implicated in the regulation of CNS polarity. Cell 75, 1417–1430
    OpenUrlCrossRefPubMedWeb of Science
    1. Ellisen L. W.,
    2. Bird J.,
    3. West D. C.,
    4. Soreng A. L.,
    5. Reynolds T. C.,
    6. Smith S. D.,
    7. Sklar J.
    (1991) Tan-1, the human homologue of the Drosophila Notch gene, is broken by chromosomal translocations in T lymphoblastic neoplasms. Cell 66, 649–661
    OpenUrlCrossRefPubMedWeb of Science
    1. Fehon R. G.,
    2. Kooh P. J.,
    3. Rebay I.,
    4. Regan C. L.,
    5. Xu T.,
    6. Muskavitch M. A. T.,
    7. Artavanis-Tsakonas S.
    (1990) Molecular interactions between the protein products of the neurogenic loci Notch and Delta, two EGF-homologous genes in Drosophila. Cell 61, 523–534
    OpenUrlCrossRefPubMedWeb of Science
    1. Fleming R. J.,
    2. Scottgale T. N.,
    3. Diederich R. J.,
    4. Artavanis-Tsakonas S.
    (1990) The gene Serrate encodes a putative EGF-like transmembrane protein essential for proper ectodermal development in Drosophila melanogaster. Genes Dev 4, 2188–2201
    OpenUrlAbstract/FREE Full Text
    1. Fortini M. E.,
    2. Artavanis-Tsakonas S.
    (1994) The suppressor of hairless protein participates in Notch receptor signaling. Cell 79, 273–282
    OpenUrlCrossRefPubMedWeb of Science
    1. Franco del Amo F.,
    2. Smith D. E.,
    3. Swiatek P. J.,
    4. Gendron-Maguire M.,
    5. Greenspan R. J.,
    6. McMahon A. P.,
    7. Gridley T.
    (1992) Expression pattern of Motch, a mouse homolog of Drosophila Notch, suggests an important role in early postimplantation mouse development. Development 115, 737–744
    OpenUrlAbstract/FREE Full Text
    1. Gavrieli Y.,
    2. Sherman Y.,
    3. Ben-Sasson S. A.
    (1992) Identification of programmed cell death in situ via specific labeling of nuclear DNA fragmentation. J. Cell Biol 119, 493–501
    OpenUrlAbstract/FREE Full Text
    1. Ghysen A.,
    2. Dambly-Chaudiere C.,
    3. Jan L. Y.,
    4. Jan Y.-N.
    (1993) Cellinteractions and gene interactions in peripheral neurogenesis. Genes Dev 7, 723–733
    OpenUrlFREE Full Text
    1. Graham A.,
    2. Heyman I.,
    3. Lumsden A.
    (1993) Even-numbered rhombomeres control the apoptotic elimination of neural crest cells from odd-numbered rhombomeres in the chick hindbrain. Development 119, 233–245
    OpenUrlAbstract
    1. Greenspan R. J.
    (1990) The Notch gene, adhesion, and developmental fate in the Drosophila embryo. New Biologist 2, 595–600
    OpenUrlPubMed
    1. Greenwald I.,
    2. Rubin G. M.
    (1992) Making a difference: the role of cell-cell interactions in establishing separate identities for equivalent cells. Cell 68, 271–281
    OpenUrlCrossRefPubMedWeb of Science
    1. Greenwald I. S.,
    2. Sternberg P. W.,
    3. Horvitz H. R.
    (1983) The lin-12 locus specifies cell fates in Caenorhabditis elegans. Cell 34, 435–444
    OpenUrlCrossRefPubMedWeb of Science
    1. Guillemot F.,
    2. Joyner A. L.
    (1993) Dynamic expression of the murine achaete-scute homolog (MASH-1) in the developing nervous system. Mech. Dev 42, 171–185
    OpenUrlCrossRefPubMedWeb of Science
    1. Guillemot F.,
    2. Lo L.-C.,
    3. Johnson J. E.,
    4. Auerbach A.,
    5. Anderson D. J.,
    6. Joyner A. L.
    (1993) Mammalian achaete-scute homolog 1 is required for the early development of olfactory and autonomic neurons. Cell 75, 463–476
    OpenUrlCrossRefPubMedWeb of Science
    1. Hartenstein V.,
    2. Posakony J. W.
    (1990) A dual function of the Notch gene in Drosophila sensillum development. Dev. Biol 142, 13–30
    OpenUrlCrossRefPubMedWeb of Science
    1. Hartenstein A. Y.,
    2. Rugendorff A.,
    3. Tepass U.,
    4. Hartenstein V.
    (1992) The fuction of the neurogenic genes during epithelial development in the Drosophila embryo. Development 116, 1203–1220
    OpenUrlAbstract/FREE Full Text
    1. Heitzler P.,
    2. Simpson P.
    (1991) The choice of cell fate in the epidermis of Drosophila. Cell 64, 1083–1092
    OpenUrlCrossRefPubMedWeb of Science
    1. Heitzler P.,
    2. Simpson P.
    (1993) Altered epidermal growth factor-like sequences provide evidence for a role of Notch as a receptor in cell fate decisions. Development 117, 1113–1123
    OpenUrlAbstract
    1. Herrmann B. G.
    (1991) Expression pattern of the Brachyury gene in whole-mount Twis / TWis mutant embryos. Development 113, 913–917
    OpenUrlAbstract
    1. Jeffs P.,
    2. Jaques K.,
    3. Osmond M.
    (1992) Cell death in cranial neural crest development. Anat. Embryol 185, 583–588
    OpenUrlPubMed
    1. Jeffs P.,
    2. Osmond M.
    (1992) A segmented pattern of cell death during development of the chick embryo. Anat. Embryol 185, 589–598
    OpenUrlPubMed
    1. Karnovsky M. J.
    (1965) A formaldehyde-glutaraldehyde fixative of high osmolarity for use in electron microscopy. J. Cell Biol 27, 137–.
    OpenUrl
    1. Keynes R. J.,
    2. Stern C. D.
    (1988) Mechanisms of vertebrate segmentation. Development 103, 413–429
    OpenUrlPubMedWeb of Science
    1. Kopan R.,
    2. Nye J. S.,
    3. Weintraub H.
    (1994) The intracellular domain of mouse Notch: a constitutively activated repressor of myogenesis directed at the basic helix-loop-helix region of MyoD. Development 120, 2385–2396
    OpenUrlAbstract/FREE Full Text
    1. Kosecki H.,
    2. Wallin J.,
    3. Wilting J.,
    4. Mizutani Y.,
    5. Kispert A.,
    6. Ebensperger C.,
    7. Herrmann B.,
    8. Christ B.,
    9. Balling R.
    (1993) A role for Pax-1 as a mediator of notochordal signals during the dorsoventral specification of vertebrae. Development 119, 649–660
    OpenUrlAbstract/FREE Full Text
    1. Lardelli M.,
    2. Lendahl U.
    (1993) Motch A and Motch B—two mouse Notch homologues coexpressed in a wide variety of tissues. Exp. Cell Res 204, 364–372
    OpenUrlCrossRefPubMedWeb of Science
    1. Lardelli M.,
    2. Dahlstrand J.,
    3. Lendahl U.
    (1994) The novel Notch homologue mouse Notch 3 lacks specific EGF-repeats and is expressed in proliferating neuroepithelium. Mech. Dev 46, 123–136
    OpenUrlCrossRefPubMedWeb of Science
    1. Lieber T.,
    2. Wesley C. S.,
    3. Alcamo E.,
    4. Hassel B.,
    5. Krane J. F.,
    6. Campos-Ortega J. A.,
    7. Young M. W.
    (1992) Single amino acid substitutions in EGF-like elements of Notch and Delta modify Drosophila development and affect cell adhesion in vitro. Neuron 9, 847–859
    OpenUrlCrossRefPubMedWeb of Science
    1. Lieber T.,
    2. Kidd S.,
    3. Alcamo E.,
    4. Corbin V.,
    5. Young M. W.
    (1993) Antineurogenic phenotypes induced by truncated Notch proteins indicate a role in signal transduction and may point to a novel function for Notch in nuclei. Genes Dev 7, 1949–1965
    OpenUrlAbstract/FREE Full Text
    1. Mansour S. L.,
    2. Thomas K. R.,
    3. Capecchi M. R.
    (1988) Disruption of the proto-oncogene int-2 in mouse embryo-derived stem cells: a general strategy for targeting mutations to non-selectable genes. Nature 336, 348–352
    OpenUrlCrossRefPubMed
    1. Meier S.
    (1984) Somite formation and its relationship to metameric patterning of the mesoderm. Cell Differentiation 14, 235–243
    OpenUrlCrossRefPubMed
    1. Mello C. C.,
    2. Draper B. W.,
    3. Priess J. R.
    (1994) The maternal genes apx-1 and glp-1 and establishment of dorsal-ventral polarity in the early C. elegans embryo. Cell 77, 95–106
    OpenUrlCrossRefPubMedWeb of Science
    1. Moens C. B.,
    2. Auerbach A. B.,
    3. Conlon R. A.,
    4. Joyner A. L.,
    5. Rossant J.
    (1992) A targeted mutation reveals a role for N- myc in branching morphogenesis in the embryonic mouse lung. Genes Dev 6, 691–704
    OpenUrlAbstract/FREE Full Text
    1. Nagy A.,
    2. Rossant J.,
    3. Nagy R.,
    4. Abramow-Newerly W.,
    5. Roder J. C.
    (1993) Derivation of completely cell culture-derived mice from early-passage embryonic stem cells. Proc. Natl. Acad. Sci. USA 90, 8424–8428
    OpenUrlAbstract/FREE Full Text
    1. Nye J. S.,
    2. Kopan R.,
    3. Axel R.
    (1994) An activated Notch suppresses neurogenesis and myogenesis but not gliogenesis in mammalian cells. Development 120, 24521–2430
    OpenUrl
    1. Reaume A. G.,
    2. Conlon R. A.,
    3. Zirngibl R.,
    4. Yamaguchi T. P.,
    5. Rossant J.
    (1992) Expression analysis of a Notch homologue in the mouse embryo. Dev. Biol 154, 377–387
    OpenUrlCrossRefPubMedWeb of Science
    1. Rebay I.,
    2. Fehon R. G.,
    3. Artavanis-Tsakonas S.
    (1993) Specific truncations of Drosophila Notch define dominant activated and dominant negative forms of the receptor. Cell 74, 319–329
    OpenUrlCrossRefPubMedWeb of Science
    1. Roehl H.,
    2. Kimble J.
    (1993) Control of cell fate in C. elegans by a glp-1 peptide consisting primarily of ankyrin repeats. Nature 364, 642–635
    OpenUrlCrossRefPubMed
    1. Sternberg P. W.
    (1988) Lateral inhibition during vulval induction in Caenorhabditis elegans. Nature 335, 551–554
    OpenUrlCrossRefPubMedWeb of Science
    1. Stifani S.,
    2. Blaumueller C. M.,
    3. Redhead N. J.,
    4. Hill R. E.,
    5. Artavanis-Tsakonas S.
    (1992) Human homologs of a Drosophila Enhancer of Split gene product define a novel family of nuclear proteins. Nature Genetics 2, 119–126
    OpenUrlCrossRefPubMedWeb of Science
    1. Struhl G.,
    2. Fitzgerald K.,
    3. Greenwald I.
    (1993) Intrinsic activity of the lin-12 and Notch intracellular domains in vivo. Cell 74, 331–345
    OpenUrlCrossRefPubMedWeb of Science
    1. Swiatek P. J.,
    2. Lindsell C. E.,
    3. del Amo F. F.,
    4. Weinmaster G.,
    5. Gridley T.
    (1994) Notch1 is essential for postimplantation development in mice. Genes Dev 8, 707–719
    OpenUrlAbstract/FREE Full Text
    1. Takada S.,
    2. Stark K. L.,
    3. Shea M. J.,
    4. Vassileva G.,
    5. McMahon J. A.,
    6. McMahon A. P.
    (1994) Wnt-3a regulates somite and tailbud formation in the mouse embryo. Genes Dev 8, 174–189
    OpenUrlAbstract/FREE Full Text
    1. Tam P. P. L.,
    2. Meier S.,
    3. Jacobson A. G.
    (1982) Differentiation of the metameric pattern in the embryonic axis of the mouse. II. Somitomeric organization of the presomitic mesoderm. Differentiation 21, 109–122
    OpenUrlCrossRefPubMedWeb of Science
    1. Tax F. E.,
    2. Yeargers J. J.,
    3. Thomas J. H.
    (1994) Sequence of C. elegans lag-2 reveals a cell-signaling domain shared with Delta and Serrate of Drosophila. Nature 368, 150–154
    OpenUrlCrossRefPubMed
    1. Vässin H.,
    2. Bremer K. A.,
    3. Knust E.,
    4. Campos-Ortega J. A.
    (1987) The neurogenic gene Delta of Drosophila melanogaster is expressed in neurogenic territories and encodes a putative transmembrane protein with EGF-like repeats. EMBO J 6, 3431–3440
    OpenUrlPubMedWeb of Science
    1. Weinmaster G.,
    2. Roberts V.,
    3. Lemke G.
    (1991) A homolog of DrosophilaNotch expressed during mammalian development. Development 113, 199–205
    OpenUrlAbstract
    1. Weinmaster G.,
    2. Roberts V. J.,
    3. Lemke G.
    (1992) Notch2 a second mammalian Notch gene. Development 116, 931–941
    OpenUrlAbstract/FREE Full Text
    1. Wolf C.,
    2. Thisse C.,
    3. Stoetzel C.,
    4. Thisse B.,
    5. Gerlinger P.,
    6. Perrin-Schmitt F.
    (1991) The M-twist gene of Mus is expressed in subsets of mesodermal cells and is closely related to the Xenopus X-twi and the Drosophila twist genes. Dev. Biol 143, 363–373
    OpenUrlCrossRefPubMedWeb of Science
    1. Yamaguchi T. P.,
    2. Conlon R. A.,
    3. Rossant J.
    (1992) Expression of the fibroblast growth factor receptor FGFR-1/flg during gastrulation and segmentation in the mouse embryo. Dev. Biol 152, 75–88
    OpenUrlCrossRefPubMedWeb of Science
    1. Yamaguchi T. P.,
    2. Harpal K.,
    3. Henkemeyer M.,
    4. Rossant J.
    (1994) fgfr-1 is required for embryonic growth and mesodermal patterning during mouse gastrulation. Genes Dev 8, 3032–3044
    OpenUrlAbstract/FREE Full Text
Back to top

 Download PDF

Email
JOURNAL ARTICLES
Notch1 is required for the coordinate segmentation of somites
R.A. Conlon, A.G. Reaume, J. Rossant
Development 1995 121: 1533-1545;
del.icio.us logo Digg logo Reddit logo Twitter logo CiteULike logo Facebook logo Google logo Mendeley logo
Citation Tools
Alerts

Article navigation

Other journals from The Company of Biologists

Advertisement

Interviews — Bénédicte Sanson and Kate Storey

Bénédicte Sanson and Kate Storey

Hear from Bénédicte Sanson, winner of the BSDB’s Cheryll Tickle medal, and Kate Storey, winner of the BSDB’s Waddington Medal, as they discuss their research, the future of the field and the importance of collaboration.

Review Commons launches

We're excited to be an affiliate journal for Review Commons, the ASAPbio/EMBO platform for high-quality journal-independent peer-review in the life sciences, which went live on 09 December.

Early-career researchers can apply for up to £2,500 to offset the cost of travel and expenses to make collaborative visits to other labs around the world. Read about Peter’s experience in Switzerland, where he joined forces with the Lutolf lab to refine a protocol for producing gastruloids.

Publishing peer review reports

To continue working towards transparency around the editorial process, Development now publishes a ‘Peer review history file’ alongside published papers. Read more about the policy and see the reports for yourself in one the first papers to publish the reports (under the ‘Info & metrics’ tab).

Development at a glance — Cell interactions in collective cell migration

Extract from the poster showing specific cell-cell interactions in metastasis.

Take a look at the latest poster and accompanying article by Denise Montell and her colleagues from the University of California, where they describe a sampling of both known and new cells that migrate collectively in vivo.