Advertisement
JOURNAL ARTICLES
Control of Drosophila adult pattern by extradenticle
S. Gonzalez-Crespo, G. Morata
Development 1995 121: 2117-2125;

Summary

The homeobox gene extradenticle (exd) acts as a cofactor of the homeotic genes in the specification of larval patterns during embryogenesis. To study its role in adult patterns, we have generated clones of mutant exd- cells and examined their effect on the different body parts. In some regions, exd- clones exhibit homeotic transformations similar to those produced by known homeotic mutations such as Ultrabithorax (Ubx), labial (lab), spineless-aristapedia (ssa) or Antennapedia (Antp). In other regions, the lack of exd causes novel homeotic transformations producing ectopic eyes and legs. Moreover, exd is also required for functions normally not associated with homeosis, such as the maintenance of the dorsoventral pattern, the specification of subpatterns in adult appendages or the arrangement of bristles in the mesonotum and genitalia. Our findings indicate that exd is critically involved in adult morphogenesis, not only in the homeotic function but also in several other developmental processes.

Reference

    1. Bryant P. J.
    (1975) Pattern formation in the imaginal wing disc of Drosophila melanogaster: Fate map, regeneration and duplication. J. Exp. Zool 193, 49–78
    OpenUrlCrossRefPubMedWeb of Science
    1. Casanova J.,
    2. Sánchez-Herrero E.,
    3. Morata G.
    (1987) Double and triple mutant combinations for the bithorax complex of Drosophila. EMBO J 6, 3103–3109
    OpenUrlPubMed
    1. Chan S.,
    2. Jaffe L.,
    3. Capovilla M.,
    4. Botas J.,
    5. Mann R. M.
    (1994) The DNA binding specificity of Ultrabithorax is modulated by cooperative interactions with Extradenticle, another homeoprotein. Cell 78, 603–615
    OpenUrlCrossRefPubMedWeb of Science
    1. Cheyette B. N. R.,
    2. Green P. J.,
    3. Martin K.,
    4. Garren H.,
    5. Hartenstein V.,
    6. Zipursky S. L.
    (1994) The Drosophila sine oculis locus encodes a homeodomain-containing protein required for the development of the entire visual system. Neuron 12, 977–996
    OpenUrlCrossRefPubMedWeb of Science
    1. Cohen S. M.,
    2. Brönner G.,
    3. Kuttner F.,
    4. Jurgens G.,
    5. Jäckle H.
    (1989) Distal-less encodes a homoeodomain protein required for limb development in Drosophila. Nature 338, 432–434
    OpenUrlCrossRefPubMed
    1. Desplan C.,
    2. Theis J.,
    3. O'Farrell P. H.
    (1985) The Drosophila developmental gene engrailed encodes a sequence specific DNA binding activity. Nature 318, 630–635
    OpenUrlCrossRefPubMed
    1. Desplan C.,
    2. Theis J.,
    3. O'Farrell P. H.
    (1988) The sequence specificity of homeodomain-DNA interaction. Cell 54, 1081–1090
    OpenUrlCrossRefPubMedWeb of Science
    1. Flegel W. A.,
    2. Singson A. W.,
    3. Margolis J. S.,
    4. Bang A. G.,
    5. Posakony J. W.,
    6. Murre C.
    (1993) Dpbx, a new homeobox gene closely related to the human proto-oncogene pbx1. Molecular structure and developmental expression. Mech. Dev 41, 155–161
    OpenUrlCrossRefPubMedWeb of Science
    1. García-Bellido A.,
    2. Merriam J. R.
    (1971) Clonal parameters of tergite development in Drosophila. Dev. Biol 26, 264–276
    OpenUrlCrossRefPubMed
    1. González-Reyes A.,
    2. Morata G.
    (1990) The developmental effect of overexpressing a Ubx product in Drosophila embryos is dependent on its interactions with other homeotic products. Cell 61, 515–522
    OpenUrlCrossRefPubMedWeb of Science
    1. Guan K.,
    2. Dixon J. E.
    (1991) Eukaryotic proteins expressed in Escherichia coli: An improved thrombin cleavage and purification procedure of fusion proteins with glutathione S -transferase. Anal. Biochem 192, 262–267
    OpenUrlCrossRefPubMedWeb of Science
    1. Haynie J. L.,
    2. Bryant P. J.
    (1986) Development of the eye-antenna imaginal disc and morphogenesis of the adult head in Drosophila melanogaster. J. Exp. Zool 237, 293–308
    OpenUrlCrossRefPubMedWeb of Science
    1. Hoey T.,
    2. Levine M.
    (1988) Divergent homeo box proteins recognize similar DNA sequences in Drosophila. Nature 332, 858–861
    OpenUrlCrossRefPubMed
    1. Kamps M. P.,
    2. Murre C.,
    3. Sun X.,
    4. Baltimore D.
    (1990) A new homeobox gene contributes the DNA binding domain of the t(1;19) translocation protein in pre-B ALL. Cell 60, 547–555
    OpenUrlCrossRefPubMedWeb of Science
    1. Kaufman T. C.,
    2. Seeger M. A.,
    3. Olsen G.
    (1990) Molecular and genetic organization of the Antennapedia gene complex of Drosophila melanogaster. Adv. Genetics 27, 309–362
    OpenUrlPubMed
    1. Kerridge S.,
    2. Morata G.
    (1982) Developmental effects of some newly induced Ultrabithorax alleles of Drosophila. J. Embryol. Exp. Morph 68, 211–234
    OpenUrlPubMedWeb of Science
    1. Lawrence P. A.,
    2. Morata G.
    (1994) Homeobox genes: Their function in Drosophila segmentation and pattern formation. Cell 78, 181–189
    OpenUrlCrossRefPubMedWeb of Science
    1. Lewis E. B.
    (1978) A gene complex controlling segmentation in Drosophila. Nature 276, 565–570
    OpenUrlCrossRefPubMed
    1. Merrill V. K. L.,
    2. Diederich R. J.,
    3. Turner F. R.,
    4. Kaufman T. C.
    (1989) A genetic and developmental analysis of mutations in labial, a gene necessary for proper head formation in Drosophila melanogaster. Dev. Biol 135, 376–391
    OpenUrlCrossRefPubMedWeb of Science
    1. Monica K.,
    2. Galili N.,
    3. Nourse J.,
    4. Saltman D.,
    5. Cleary M. L.
    (1991) PBX2 and PBX3, new homeobox genes with extensive homology to the human proto-oncogene PBX1. Mol. Cell. Biol 11, 6149–6157
    OpenUrlAbstract/FREE Full Text
    1. Morata G.,
    2. Lawrence P. A.
    (1977) Homoeotic genes, compartments and cell determination in Drosophila. Nature 265, 211–216
    OpenUrlCrossRefPubMed
    1. Morata G.,
    2. Lawrence P. A.
    (1979) Development of the eye-antenna imaginal disc of Drosophila. Dev. Biol 70, 355–371
    OpenUrlCrossRefPubMed
    1. Morata G.,
    2. Ripoll P.
    (1975) Minutes: Mutants of Drosophila autonomously affecting cell division rata. Dev. Biol 42, 211–221
    OpenUrlCrossRefPubMedWeb of Science
    1. Nourse J.,
    2. Mellentin J. D.,
    3. Galili N.,
    4. Wilkinson J.,
    5. Stanbridge E.,
    6. Smith S. D.,
    7. Cleary M. L.
    (1990) Chromosomal translocation t(1;19) results in synthesis of a homeobox fusion mRNA that codes for a potential chimeric transcription factor. Cell 60, 535–545
    OpenUrlCrossRefPubMedWeb of Science
    1. Peifer M.,
    2. Wieschaus E.
    (1990) Mutations in the Drosophila gene extradenticle affect the way specific homeo domain proteins regulate segmental identity. Genes Dev 4, 1209–1223
    OpenUrlAbstract/FREE Full Text
    1. Postlethwait J. H.,
    2. Girton J. R.
    (1974) Development in genetic mosaics of aristapedia, a homoeotic mutant of Drosophila melanogaster. Genetics 76, 767–774
    OpenUrlAbstract/FREE Full Text
    1. Quiring R.,
    2. Walldorf U.,
    3. Kloter U.,
    4. Gehring W. J.
    (1994) Homology of the eyeless gene of Drosophila to the Small eye gene in mice and Aniridia in humans. Science 265, 785–789
    OpenUrlAbstract/FREE Full Text
    1. Rauskolb C.,
    2. Peifer M.,
    3. Wieschaus E.
    (1993) extradenticle, a regulator of homeotic gene activity, is a homolog of the homeobox-containing human proto-oncogene pbx1. Cell 74, 1101–1112
    OpenUrlCrossRefPubMedWeb of Science
    1. Sánchez-Herrero E.,
    2. Guerrero I.,
    3. Sampedro J.,
    4. González-Reyes A.
    (1994) Developmental consequences of unrestricted expression of the abd-A gene of Drosophila. Mech. Dev 46, 153–167
    OpenUrlCrossRefPubMedWeb of Science
    1. Sánchez-Herrero E.,
    2. Vernos I.,
    3. Marco R.,
    4. Morata G.
    (1985) Genetic organization of Drosophila bithorax complex. Nature 313, 108–113
    OpenUrlCrossRefPubMed
    1. Schneuwly S.,
    2. Klemenz R.,
    3. Gehring W. J.
    (1987) Redesigning the body plan of Drosophila by ectopic expression of the homoeotic gene Antennapedia. Nature 325, 816–818
    OpenUrlCrossRefPubMed
    1. Smith D. B.,
    2. Johnson K. S.
    (1988) Single-step purification of polypeptides expressed in E. coli as fusions with glutathione S-transferase. Gene 67, 31–40
    OpenUrlCrossRefPubMedWeb of Science
    1. Thali M.,
    2. Muller M. M.,
    3. DeLorenzi M.,
    4. Matthias P.,
    5. Bienz M.
    (1988) Drosophila homoeotic genes encode transcriptional activators similar to mammalian OTF-2. Nature 336, 598–601
    OpenUrlCrossRefPubMedWeb of Science
    1. van Dijk M. A.,
    2. Murre C.
    (1994) extradenticle raises the DNA binding specificity of homeotic selector gene products. Cell 78, 617–624
    OpenUrlCrossRefPubMedWeb of Science
    1. Wilson D. S.,
    2. Desplan C.
    (1995) Cooperating to be different. Curr. Biol 5, 32–34
    OpenUrlCrossRefPubMedWeb of Science
    1. Wirz J.,
    2. Fessler L. I.,
    3. Gehring W. J.
    (1986) Localization of the Antennapedia protein in Drosophila embryos and imaginal discs. EMBO J 5, 3327–3334
    OpenUrlPubMedWeb of Science
Back to top

 Download PDF

Email
JOURNAL ARTICLES
Control of Drosophila adult pattern by extradenticle
S. Gonzalez-Crespo, G. Morata
Development 1995 121: 2117-2125;
del.icio.us logo Digg logo Reddit logo Twitter logo CiteULike logo Facebook logo Google logo Mendeley logo
Citation Tools
Alerts

Article navigation

Other journals from The Company of Biologists

Advertisement

An interview with Swathi Arur

Swathi Arur joined the team at Development as an Academic Editor in 2020. Her lab uses multidisciplinary approaches to understand female germline development and fertility. We met with her over Zoom to hear more about her life, her career and her love for C. elegans.

Jim Wells and Hanna Mikkola join our team of Editors

We are pleased to welcome James (Jim) Wells and Hanna Mikkola to our team of Editors. Jim joins us a new Academic Editor, taking over from Gordan Keller, and Hanna joins our team of Associate Editors. Find out more about their research interests and areas of expertise.

New funding scheme supports sustainable events

As part of our Sustainable Conferencing Initiative, we are pleased to announce funding for organisers that seek to reduce the environmental footprint of their event. The next deadline to apply for a Scientific Meeting grant is 26 March 2021.

Read & Publish participation continues to grow

“I’d heard of Read & Publish deals and knew that many universities, including mine, had signed up to them but I had not previously understood the benefits that these deals bring to authors who work at those universities.”

Professor Sally Lowell (University of Edinburgh) shares her experience of publishing Open Access as part of our growing Read & Publish initiative. We now have over 150 institutions in 15 countries and four library consortia taking part – find out more and view our full list of participating institutions.

Upcoming special issues

Imaging Development, Stem Cells and Regeneration
Submission deadline: 30 March 2021
Publication: mid-2021

The Immune System in Development and Regeneration
Guest editors: Florent Ginhoux and Paul Martin
Submission deadline: 1 September 2021
Publication: Spring 2022

Both special issues welcome Review articles as well as Research articles, and will be widely promoted online and at key global conferences.

Development presents...

Our successful webinar series continues into 2021, with early-career researchers presenting their papers and a chance to virtually network with the developmental biology community afterwards. Here, Brandon Carpenter talks about how inherited histone methylation defines the germline versus soma decision in C. elegans. 

Sign up to join our next session:

10 March
Time: TBC
Chaired by: Thomas Lecuit