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JOURNAL ARTICLES
The existence of inositol 1,4,5-trisphosphate and ryanodine receptors in mature bovine oocytes
C. Yue, K.L. White, W.A. Reed, T.D. Bunch
Development 1995 121: 2645-2654;
C. Yue
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K.L. White
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W.A. Reed
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T.D. Bunch
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Summary

Intracellular Ca2+ (Ca2+i) transients during fertilization are critical to the activation of eggs in all species studied. Activation of both the inositol 1,4,5-trisphosphate (IP3) receptor (IP3R) and ryanodine receptor (RYR) are responsible for the calcium oscillations during fertilization in sea urchin eggs. Using in vitro matured bovine oocytes loaded with Fura-2 AM ester as Ca2+i indicator, we addressed whether IP3Rs and RYRs coexist in mammalian eggs. Our results indicate that microinjection of 50–250 nM IP3 or 10–20 mM caffeine, 100–200 microM ryanodine and 4–8 microM cyclic ADP-ribose all induced Ca2+i release. The Ca2+i release induced by 250 nM IP3 could only be inhibited by prior injection of 1 mg/ml heparin which was overcome by continuous injection of IP3 to 1 microM. Prior injection of either 50 microM ruthenium red, 50 microM procaine or 1 % vehicle medium (VM) did not affect the Ca2+i release induced by IP3. Prior injection of heparin or VM did not affect the Ca2+i release induced by 10–20 mM caffeine or 200 microM ryanodine, but prior injection of 50 microM ruthenium red or procaine completely inhibited the effect of 10–20 mM caffeine. In addition, continuous injection of caffeine up to 40 mM overcame the inhibitory effect of ruthenium red or procaine. The same 50 microM concentration of ruthenium red or procaine only partially blocked the effect of 200 microM ryanodine, but 200 microM ruthenium red or procaine completely blocked the effect of 200 microM ryanodine.(ABSTRACT TRUNCATED AT 250 WORDS)

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JOURNAL ARTICLES
The existence of inositol 1,4,5-trisphosphate and ryanodine receptors in mature bovine oocytes
C. Yue, K.L. White, W.A. Reed, T.D. Bunch
Development 1995 121: 2645-2654;
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JOURNAL ARTICLES
The existence of inositol 1,4,5-trisphosphate and ryanodine receptors in mature bovine oocytes
C. Yue, K.L. White, W.A. Reed, T.D. Bunch
Development 1995 121: 2645-2654;

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