Skip to main content
Advertisement

Main menu

  • Home
  • Articles
    • Accepted manuscripts
    • Issue in progress
    • Latest complete issue
    • Issue archive
    • Archive by article type
    • Special issues
    • Subject collections
    • Sign up for alerts
  • About us
    • About Development
    • About the Node
    • Editors and Board
    • Editor biographies
    • Travelling Fellowships
    • Grants and funding
    • Journal Meetings
    • Workshops
    • The Company of Biologists
    • Journal news
  • For authors
    • Submit a manuscript
    • Aims and scope
    • Presubmission enquiries
    • Article types
    • Manuscript preparation
    • Cover suggestions
    • Editorial process
    • Promoting your paper
    • Open Access
    • Biology Open transfer
  • Journal info
    • Journal policies
    • Rights and permissions
    • Media policies
    • Reviewer guide
    • Sign up for alerts
  • Contacts
    • Contacts
    • Subscriptions
    • Feedback
  • COB
    • About The Company of Biologists
    • Development
    • Journal of Cell Science
    • Journal of Experimental Biology
    • Disease Models & Mechanisms
    • Biology Open

User menu

  • Log in
  • Log out

Search

  • Advanced search
Development
  • COB
    • About The Company of Biologists
    • Development
    • Journal of Cell Science
    • Journal of Experimental Biology
    • Disease Models & Mechanisms
    • Biology Open

supporting biologistsinspiring biology

Development

  • Log in
Advanced search

RSS  Twitter  Facebook  YouTube 

  • Home
  • Articles
    • Accepted manuscripts
    • Issue in progress
    • Latest complete issue
    • Issue archive
    • Archive by article type
    • Special issues
    • Subject collections
    • Sign up for alerts
  • About us
    • About Development
    • About the Node
    • Editors and Board
    • Editor biographies
    • Travelling Fellowships
    • Grants and funding
    • Journal Meetings
    • Workshops
    • The Company of Biologists
    • Journal news
  • For authors
    • Submit a manuscript
    • Aims and scope
    • Presubmission enquiries
    • Article types
    • Manuscript preparation
    • Cover suggestions
    • Editorial process
    • Promoting your paper
    • Open Access
    • Biology Open transfer
  • Journal info
    • Journal policies
    • Rights and permissions
    • Media policies
    • Reviewer guide
    • Sign up for alerts
  • Contacts
    • Contacts
    • Subscriptions
    • Feedback
JOURNAL ARTICLES
Analysis of FGF function in normal and no tail zebrafish embryos reveals separate mechanisms for formation of the trunk and the tail
K. Griffin, R. Patient, N. Holder
Development 1995 121: 2983-2994;
K. Griffin
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
R. Patient
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
N. Holder
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • Article
  • Info & metrics
  • PDF
Loading

Summary

To analyse the roles of FGF activity and brachyury during gastrulation we have directly compared the consequences of inhibition of FGF-receptor signalling with the phenotype of the zebrafish brachyury mutant, no tail (ntl). We show that expression of ntl is regulated by FGF and that inhibition of FGF receptor-signalling leads to complete loss of the trunk and tail. Since the ntl mutant lacks the tail and notochord but has an otherwise normal trunk, this demonstrates that trunk development is dependent upon an unidentified gene, or set of genes, referred to as no trunk (ntk) which is regulated by FGF. We propose a model to explain the FGF-dependent regulation of ntl and ntk that accounts for the above phenotypes. Consistent with this model, over-expression of eFGF led to suppression of anterior fates and development of trunk and tail derivatives only. In addition, widespread activation of convergence and extension movements resulted in the formation of multiple axis-like structures. Expression of eve1 and cad1 was also regulated by FGF activity, suggesting that during gastrulation FGF activity is normally restricted to the germ ring where these genes, and ntl, are expressed. Taken together these data suggest that the germ ring acts as a posteriorising centre during AP patterning, mediated by FGF activity in this tissue.

Reference

    1. Alberga A.,
    2. Boulay J. L.,
    3. Kempe E.,
    4. Dennefeld C.,
    5. Haenlin M.
    (1991) The snail gene required for mesoderm formation in Drosophila is expressed dynamically in derivatives of all three germ layers. Development 111, 983–992
    OpenUrlAbstract/FREE Full Text
    1. Amaya E.,
    2. Musci T. J.,
    3. Kirschner M. W.
    (1991) Expression of a dominant negative mutant of the FGF receptor disrupts mesoderm formation in Xenopus embryos. Cell 66, 257–270
    OpenUrlCrossRefPubMedWeb of Science
    1. Amaya E.,
    2. Stein P. A.,
    3. Musci T. J.,
    4. Kirschner M. W.
    (1993) FGF signalling in the early specification of mesoderm in Xenopus. Development 118, 477–487
    OpenUrlAbstract
    1. Barro O.,
    2. Joly C.,
    3. Condamine H.,
    4. Boulekbache H.
    (1994) Widespread expression of the Xenopus homeobox gene Xhox3 in zebrafish eggs causes a disruption of the anterior-posterior axis. Int. J. Dev. Biol 38, 613–622
    OpenUrlPubMed
    1. Beddington R. S. P.,
    2. Smith J. C.
    (1993) Control of vertebrate gastrulation: inducing signals and responding genes. Curr. Op. Genet. Dev 3, 655–661
    OpenUrlCrossRefPubMed
    1. Chesley P.
    (1935) Development of the short-tailed mutant in the house mouse. J. Exp. Zool 70, 429–435
    OpenUrlCrossRef
    1. Christian J. L.,
    2. Olson D. J.,
    3. Moon R. T.
    (1992) Xwnt-8 modifies the character of mesoderm induced by bFGF in isolated Xenopus ectoderm. EMBO J 11, 33–41
    OpenUrlPubMedWeb of Science
    1. Cornell R. A.,
    2. Kimelman D.
    (1994) Activin-mediated mesoderm induction requires FGF. Development 120, 453–462
    OpenUrlAbstract
    1. Cunliffe V.,
    2. Smith J. C.
    (1992) Ectopic mesoderm formation in Xenopus embryos caused by widespread expression of a brachyury homologue. Nature 358, 427–430
    OpenUrlCrossRefPubMed
    1. Driever W.,
    2. Stemple D.,
    3. Schier A.,
    4. Solnica-Krezel L.
    (1994) Zebrafish: genetic tools for studying development. Trends Genet 4, 152–159
    1. Halpern M. E.,
    2. Ho R. K.,
    3. Walker C.,
    4. Kimmel C. B.
    (1993) Induction of muscle pioneers and floor plate is distinguished by the zebrafish no tail mutation. Cell 75, 99–112
    OpenUrlCrossRefPubMedWeb of Science
    1. Hammerschmidt M.,
    2. Nusslein-Volhard C.
    (1994) The expression of a zebrafish gene homologous to Drosophila snail suggests a conserved function in invertebrate and vertebrate gastrulation. Development 119, 1107–1118
    OpenUrlAbstract
    1. Harding K.,
    2. Rushlow C.,
    3. Doyle H. J.,
    4. Hoey T.,
    5. Levine M.
    (1986) Cross regulatory interactions among pair rule genes in Drosophila. Science 233, 953–959
    OpenUrlAbstract/FREE Full Text
    1. Inoue A.,
    2. Takahashi M.,
    3. Hatta K.,
    4. Hotta Y.,
    5. Okamoto H.
    (1994) Developmental regulation of islet-1 mRNA expression during neuronal differentiation in embryonic zebrafish. Dev. Dynamics 199, 1–11
    OpenUrlPubMedWeb of Science
    1. Isaacs H. V.,
    2. Tannahill D.,
    3. Slack J. M. W.
    (1992) Expression of a novel FGF in the Xenopus embryo. A new candidate inducing factor for mesoderm formation and anteroposterior specification. Development 114, 711–720
    OpenUrlAbstract
    1. Isaacs H. V.,
    2. Pownall M. E.,
    3. Slack J. M. W.
    (1994) eFGF regulates Xbra expression during Xenopus gastrulation. EMBO J 13, 4469–4481
    OpenUrlPubMedWeb of Science
    1. Joly J. S.,
    2. Maury M.,
    3. Joly C.,
    4. Duprey P.,
    5. Boulekbache H.,
    6. Condamine H.
    (1992) Expression of the zebrafish caudal homeobox gene correlates with the establishment of posterior cell lineages at gastrulation. Diff 50, 75–87
    OpenUrlCrossRefPubMed
    1. Joly J. S.,
    2. Joly C.,
    3. Schulte-Merker S.,
    4. Boulekbache H.,
    5. Condamine H.
    (1993) The ventral and posterior expression of the zebrafish homeobox gene eve1 is perturbed in dorsalized and mutant embryos. Development 119, 1261–1275
    OpenUrlAbstract
    1. Keller R.,
    2. Danilchik M.
    (1988) Regional expression, pattern and timing of convergence and extension during gastrulation of Xenopus laevis. Development 103, 193–209
    OpenUrlAbstract
    1. Kimelman D.,
    2. Kirschner M.
    (1987) Synergistic induction of mesoderm by FGF and TGFb and the identification of an mRNA coding for FGF in the early Xenopus embryo. Cell 51, 869–877
    OpenUrlCrossRefPubMedWeb of Science
    1. Krauss S.,
    2. Johansen T.,
    3. Korzh V.,
    4. Fjose A.
    (1991) Expression patternof zebrafish pax genes suggest a role in early brain regionalisation. Nature 353, 267–270
    OpenUrlCrossRefPubMed
    1. Krauss S.,
    2. Concordet J.-P.,
    3. Ingham P. W.
    (1993) A functionally conserved homolog of the Drosophila segment polarity gene hh is expressed in tissues with polarizing activity in zebrafish embryos. Cell 75, 1431–1444
    OpenUrlCrossRefPubMedWeb of Science
    1. Krieg P.,
    2. Melton D.
    (1984) Functional messenger RNAs are produced by SP6 in vitro transcription of cloned cDNAs. Nucl. Acid Res 12, 7057–7070
    OpenUrlAbstract/FREE Full Text
    1. Mlodzik M.,
    2. Fjose A.,
    3. Gehring W. J.
    (1985) Isolation of caudal, a Drosophila homeobox-containing gene with maternal expression, whose transcripts form a concentration gradient at the preblastoderm stage. EMBO J 4, 2961–2969
    OpenUrlPubMedWeb of Science
    1. Mlodzik M.,
    2. Gibson G.,
    3. Gehring W. J.
    (1990) Effects of ectopic expression of caudal during Drosophila development. Development 109, 271–277
    OpenUrlAbstract
    1. Mullins M. C.,
    2. Hammerschmidt M.,
    3. Haffter P.,
    4. Nusslein-Volhard C.
    (1994) Large-scale mutagenesis in the zebrafish: in search of genes controlling development in a vertebrate. Curr. Biol 4, 189–202
    OpenUrlCrossRefPubMedWeb of Science
    1. Oxtoby E.,
    2. Jowett T.
    (1993) Cloning of the zebrafish krox-20 gene (krx-20) and its expression during hindbrain development. Nucl. Acids Res 21, 1087–1095
    OpenUrlAbstract/FREE Full Text
    1. Pavlath G. K.,
    2. Rich K.,
    3. Webster S. G.,
    4. Blau H. M.
    (1989) Localization of muscle gene products in nuclear domains. Nature 337, 570–573
    OpenUrlCrossRefPubMed
    1. Reichman-Fried M.,
    2. Dickson B.,
    3. Hafen E.,
    4. Shilo B. Z.
    (1994) Elucidation of the role of breathless, a Drosophila FGF receptor homolog, in tracheal cell migration. Genes Dev 8, 428–439
    OpenUrlAbstract/FREE Full Text
    1. Ruiz i Altaba A.,
    2. Melton D. A.
    (1989) Interaction between peptide growth factors and homeobox genes in the establishment of antero-posterior polarity in frog embryos. Nature 341, 33–38
    OpenUrlCrossRefPubMed
    1. Ruiz i Altaba A.,
    2. Melton D. A.
    (1989) Involvement of the Xenopus homeobox gene Xhox3 in pattern formation along the anterior-posterior axis. Cell 57, 317–326
    OpenUrlCrossRefPubMedWeb of Science
    1. Schulte-Merker S.,
    2. Ho R. K.,
    3. Nusslein-Volhard C.,
    4. Herrmann B. G.
    (1992) The protein product of the zebrafish homologue of the T gene isexpressed in nuclei of the germ ring and the notochord of the early embryo. Development 116, 1021–1032
    OpenUrlAbstract/FREE Full Text
    1. Schulte-Merker S.,
    2. van Eeden F.,
    3. Halpern M. E.,
    4. Kimmel C. B.,
    5. Nusslein-Volhard C.
    (1994) no tail (ntl) is the zebrafish homologue of the mouse T (brachyury) gene. Development 120, 1009–1015
    OpenUrlAbstract
    1. Schulte-Merker S.,
    2. Smith J. C.
    (1995) Mesoderm formation in response to Brachyury requires FGF signalling. Curr. Biol 5, 62–67
    OpenUrlCrossRefPubMedWeb of Science
    1. Schägger H.,
    2. Von Jagow G.
    (1987) Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDa. Analyt. Biochem 166, 368–379
    OpenUrlCrossRefPubMedWeb of Science
    1. Shishido E.,
    2. Higashijima S. I.,
    3. Emori Y.,
    4. Saigo K.
    (1993) Tw FGF-receptor homologues of Drosophila: one is expressed in mesodermal primordium in early embryos. Development 117, 751–761
    OpenUrlAbstract
    1. Slack J. M. W.,
    2. Darlington B. G.,
    3. Heath J. K.,
    4. Godsave S. F.
    (1987) Mesoderm induction in early Xenopus embryos by heparin-binding growth factors. Nature 326, 197–200
    OpenUrlCrossRefPubMed
    1. Slack J. M. W.
    (1994) Inducing factors in Xenopus early embryos. Curr. Biol 4, 116–126
    OpenUrlCrossRefPubMedWeb of Science
    1. Smith J. C.,
    2. Price B. M. J.,
    3. Green J. B. A.,
    4. Weigel D.,
    5. Herrmann B. G.
    (1991) Expression of the Xenopus homologue of Brachyury (T) is an immediate-early response to mesoderm induction. Cell 67, 79–87
    OpenUrlCrossRefPubMedWeb of Science
    1. Stachel S. E.,
    2. Grunwald D. J.,
    3. Myers P. Z.
    (1993) Lithium perturbation and goosecoid expression identify a dorsal specification pathway in the pregastrula zebrafish. Development 117, 1261–1274
    OpenUrlAbstract
    1. Stott D.,
    2. Kispert A.,
    3. Herrmann B. G.
    (1993) Rescue of the tail defect of Brachyury mice. Genes Dev 7, 197–203
    OpenUrlAbstract/FREE Full Text
    1. Thisse C.,
    2. Thisse B.,
    3. Schilling T. F.,
    4. Postlethwait J. H.
    (1994) Structure of the zebrafish snail1 geneand its expression in wild-type, spadetail and no tail mutant embryos. Development 119, 1203–1215
    OpenUrlAbstract
    1. Xu Q.,
    2. Holder N. H.,
    3. Patient R.,
    4. Wilson S. W.
    (1994) Spatially regulated expression of three receptor tyrosine kinase genes during gastrulation in the zebrafish. Development 120, 287–299
    OpenUrlAbstract
Previous ArticleNext Article
Back to top
Previous ArticleNext Article

This Issue

 Download PDF

Email

Thank you for your interest in spreading the word on Development.

NOTE: We only request your email address so that the person you are recommending the page to knows that you wanted them to see it, and that it is not junk mail. We do not capture any email address.

Enter multiple addresses on separate lines or separate them with commas.
Analysis of FGF function in normal and no tail zebrafish embryos reveals separate mechanisms for formation of the trunk and the tail
(Your Name) has sent you a message from Development
(Your Name) thought you would like to see the Development web site.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Share
JOURNAL ARTICLES
Analysis of FGF function in normal and no tail zebrafish embryos reveals separate mechanisms for formation of the trunk and the tail
K. Griffin, R. Patient, N. Holder
Development 1995 121: 2983-2994;
del.icio.us logo Digg logo Reddit logo Twitter logo CiteULike logo Facebook logo Google logo Mendeley logo
Citation Tools
JOURNAL ARTICLES
Analysis of FGF function in normal and no tail zebrafish embryos reveals separate mechanisms for formation of the trunk and the tail
K. Griffin, R. Patient, N. Holder
Development 1995 121: 2983-2994;

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
Alerts

Please log in to add an alert for this article.

Sign in to email alerts with your email address

Article navigation

  • Top
  • Article
  • Info & metrics
  • PDF

Related articles

Cited by...

More in this TOC section

  • Morphogenetic cell movements in the middle region of the dermomyotome dorsomedial lip associated with patterning and growth of the primary epaxial myotome
  • Germline and developmental roles of the nuclear transport factor importin (α)3 in C. elegans
  • Monofocal origin of telencephalic oligodendrocytes in the anterior entopeduncular area of the chick embryo
Show more JOURNAL ARTICLES

Similar articles

Other journals from The Company of Biologists

Journal of Cell Science

Journal of Experimental Biology

Disease Models & Mechanisms

Biology Open

Advertisement

Kathryn Virginia Anderson (1952-2020)

Developmental geneticist Kathryn Anderson passed away at home on 30 November 2020. Tamara Caspary, a former postdoc and friend, remembers Kathryn and her remarkable contribution to developmental biology.


Zooming into 2021

In a new Editorial, Editor-in-Chief James Briscoe and Executive Editor Katherine Brown reflect on the triumphs and tribulations of the last 12 months, and look towards a hopefully calmer and more predictable year.


Read & Publish participation extends worldwide

Over 60 institutions in 12 countries are now participating in our Read & Publish initiative. Here, James Briscoe explains what this means for his institution, The Francis Crick Institute. Find out more and view our full list of participating institutions.


Upcoming special issues

Imaging Development, Stem Cells and Regeneration
Submission deadline: 30 March 2021
Publication: mid-2021

The Immune System in Development and Regeneration
Guest editors: Florent Ginhoux and Paul Martin
Submission deadline: 1 September 2021
Publication: Spring 2022

Both special issues welcome Review articles as well as Research articles, and will be widely promoted online and at key global conferences.


Development presents...

Our successful webinar series continues into 2021, with early-career researchers presenting their papers and a chance to virtually network with the developmental biology community afterwards. Sign up to join our next session:

10 February
Time: 13:00 (GMT)
Chaired by: preLights

Articles

  • Accepted manuscripts
  • Issue in progress
  • Latest complete issue
  • Issue archive
  • Archive by article type
  • Special issues
  • Subject collections
  • Sign up for alerts

About us

  • About Development
  • About the Node
  • Editors and board
  • Editor biographies
  • Travelling Fellowships
  • Grants and funding
  • Journal Meetings
  • Workshops
  • The Company of Biologists

For authors

  • Submit a manuscript
  • Aims and scope
  • Presubmission enquiries
  • Article types
  • Manuscript preparation
  • Cover suggestions
  • Editorial process
  • Promoting your paper
  • Open Access
  • Biology Open transfer

Journal info

  • Journal policies
  • Rights and permissions
  • Media policies
  • Reviewer guide
  • Sign up for alerts

Contact

  • Contact Development
  • Subscriptions
  • Advertising
  • Feedback

 Twitter   YouTube   LinkedIn

© 2021   The Company of Biologists Ltd   Registered Charity 277992